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The miRNA-mRNA Regulatory Modules of Pinus massoniana Lamb. in Response to Drought Stress

Masson pine (Pinus massoniana Lamb.) is a major fast-growing woody tree species and pioneer species for afforestation in barren sites in southern China. However, the regulatory mechanism of gene expression in P. massoniana under drought remains unclear. To uncover candidate microRNAs, their expressi...

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Autores principales: Chen, Xinhua, Chen, Hu, Shen, Tengfei, Luo, Qunfeng, Xu, Meng, Yang, Zhangqi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10572226/
https://www.ncbi.nlm.nih.gov/pubmed/37834103
http://dx.doi.org/10.3390/ijms241914655
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author Chen, Xinhua
Chen, Hu
Shen, Tengfei
Luo, Qunfeng
Xu, Meng
Yang, Zhangqi
author_facet Chen, Xinhua
Chen, Hu
Shen, Tengfei
Luo, Qunfeng
Xu, Meng
Yang, Zhangqi
author_sort Chen, Xinhua
collection PubMed
description Masson pine (Pinus massoniana Lamb.) is a major fast-growing woody tree species and pioneer species for afforestation in barren sites in southern China. However, the regulatory mechanism of gene expression in P. massoniana under drought remains unclear. To uncover candidate microRNAs, their expression profiles, and microRNA-mRNA interactions, small RNA-seq was used to investigate the transcriptome from seedling roots under drought and rewatering in P. massoniana. A total of 421 plant microRNAs were identified. Pairwise differential expression analysis between treatment and control groups unveiled 134, 156, and 96 differential expressed microRNAs at three stages. These constitute 248 unique microRNAs, which were subsequently categorized into six clusters based on their expression profiles. Degradome sequencing revealed that these 248 differentially expressed microRNAs targeted 2069 genes. Gene Ontology enrichment analysis suggested that these target genes were related to translational and posttranslational regulation, cell wall modification, and reactive oxygen species scavenging. miRNAs such as miR482, miR398, miR11571, miR396, miR166, miRN88, and miRN74, along with their target genes annotated as F-box/kelch-repeat protein, 60S ribosomal protein, copper-zinc superoxide dismutase, luminal-binding protein, S-adenosylmethionine synthase, and Early Responsive to Dehydration Stress may play critical roles in drought response. This study provides insights into microRNA responsive to drought and rewatering in Masson pine and advances the understanding of drought tolerance mechanisms in Pinus.
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spelling pubmed-105722262023-10-14 The miRNA-mRNA Regulatory Modules of Pinus massoniana Lamb. in Response to Drought Stress Chen, Xinhua Chen, Hu Shen, Tengfei Luo, Qunfeng Xu, Meng Yang, Zhangqi Int J Mol Sci Article Masson pine (Pinus massoniana Lamb.) is a major fast-growing woody tree species and pioneer species for afforestation in barren sites in southern China. However, the regulatory mechanism of gene expression in P. massoniana under drought remains unclear. To uncover candidate microRNAs, their expression profiles, and microRNA-mRNA interactions, small RNA-seq was used to investigate the transcriptome from seedling roots under drought and rewatering in P. massoniana. A total of 421 plant microRNAs were identified. Pairwise differential expression analysis between treatment and control groups unveiled 134, 156, and 96 differential expressed microRNAs at three stages. These constitute 248 unique microRNAs, which were subsequently categorized into six clusters based on their expression profiles. Degradome sequencing revealed that these 248 differentially expressed microRNAs targeted 2069 genes. Gene Ontology enrichment analysis suggested that these target genes were related to translational and posttranslational regulation, cell wall modification, and reactive oxygen species scavenging. miRNAs such as miR482, miR398, miR11571, miR396, miR166, miRN88, and miRN74, along with their target genes annotated as F-box/kelch-repeat protein, 60S ribosomal protein, copper-zinc superoxide dismutase, luminal-binding protein, S-adenosylmethionine synthase, and Early Responsive to Dehydration Stress may play critical roles in drought response. This study provides insights into microRNA responsive to drought and rewatering in Masson pine and advances the understanding of drought tolerance mechanisms in Pinus. MDPI 2023-09-28 /pmc/articles/PMC10572226/ /pubmed/37834103 http://dx.doi.org/10.3390/ijms241914655 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chen, Xinhua
Chen, Hu
Shen, Tengfei
Luo, Qunfeng
Xu, Meng
Yang, Zhangqi
The miRNA-mRNA Regulatory Modules of Pinus massoniana Lamb. in Response to Drought Stress
title The miRNA-mRNA Regulatory Modules of Pinus massoniana Lamb. in Response to Drought Stress
title_full The miRNA-mRNA Regulatory Modules of Pinus massoniana Lamb. in Response to Drought Stress
title_fullStr The miRNA-mRNA Regulatory Modules of Pinus massoniana Lamb. in Response to Drought Stress
title_full_unstemmed The miRNA-mRNA Regulatory Modules of Pinus massoniana Lamb. in Response to Drought Stress
title_short The miRNA-mRNA Regulatory Modules of Pinus massoniana Lamb. in Response to Drought Stress
title_sort mirna-mrna regulatory modules of pinus massoniana lamb. in response to drought stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10572226/
https://www.ncbi.nlm.nih.gov/pubmed/37834103
http://dx.doi.org/10.3390/ijms241914655
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