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The miRNA-mRNA Regulatory Modules of Pinus massoniana Lamb. in Response to Drought Stress
Masson pine (Pinus massoniana Lamb.) is a major fast-growing woody tree species and pioneer species for afforestation in barren sites in southern China. However, the regulatory mechanism of gene expression in P. massoniana under drought remains unclear. To uncover candidate microRNAs, their expressi...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10572226/ https://www.ncbi.nlm.nih.gov/pubmed/37834103 http://dx.doi.org/10.3390/ijms241914655 |
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author | Chen, Xinhua Chen, Hu Shen, Tengfei Luo, Qunfeng Xu, Meng Yang, Zhangqi |
author_facet | Chen, Xinhua Chen, Hu Shen, Tengfei Luo, Qunfeng Xu, Meng Yang, Zhangqi |
author_sort | Chen, Xinhua |
collection | PubMed |
description | Masson pine (Pinus massoniana Lamb.) is a major fast-growing woody tree species and pioneer species for afforestation in barren sites in southern China. However, the regulatory mechanism of gene expression in P. massoniana under drought remains unclear. To uncover candidate microRNAs, their expression profiles, and microRNA-mRNA interactions, small RNA-seq was used to investigate the transcriptome from seedling roots under drought and rewatering in P. massoniana. A total of 421 plant microRNAs were identified. Pairwise differential expression analysis between treatment and control groups unveiled 134, 156, and 96 differential expressed microRNAs at three stages. These constitute 248 unique microRNAs, which were subsequently categorized into six clusters based on their expression profiles. Degradome sequencing revealed that these 248 differentially expressed microRNAs targeted 2069 genes. Gene Ontology enrichment analysis suggested that these target genes were related to translational and posttranslational regulation, cell wall modification, and reactive oxygen species scavenging. miRNAs such as miR482, miR398, miR11571, miR396, miR166, miRN88, and miRN74, along with their target genes annotated as F-box/kelch-repeat protein, 60S ribosomal protein, copper-zinc superoxide dismutase, luminal-binding protein, S-adenosylmethionine synthase, and Early Responsive to Dehydration Stress may play critical roles in drought response. This study provides insights into microRNA responsive to drought and rewatering in Masson pine and advances the understanding of drought tolerance mechanisms in Pinus. |
format | Online Article Text |
id | pubmed-10572226 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105722262023-10-14 The miRNA-mRNA Regulatory Modules of Pinus massoniana Lamb. in Response to Drought Stress Chen, Xinhua Chen, Hu Shen, Tengfei Luo, Qunfeng Xu, Meng Yang, Zhangqi Int J Mol Sci Article Masson pine (Pinus massoniana Lamb.) is a major fast-growing woody tree species and pioneer species for afforestation in barren sites in southern China. However, the regulatory mechanism of gene expression in P. massoniana under drought remains unclear. To uncover candidate microRNAs, their expression profiles, and microRNA-mRNA interactions, small RNA-seq was used to investigate the transcriptome from seedling roots under drought and rewatering in P. massoniana. A total of 421 plant microRNAs were identified. Pairwise differential expression analysis between treatment and control groups unveiled 134, 156, and 96 differential expressed microRNAs at three stages. These constitute 248 unique microRNAs, which were subsequently categorized into six clusters based on their expression profiles. Degradome sequencing revealed that these 248 differentially expressed microRNAs targeted 2069 genes. Gene Ontology enrichment analysis suggested that these target genes were related to translational and posttranslational regulation, cell wall modification, and reactive oxygen species scavenging. miRNAs such as miR482, miR398, miR11571, miR396, miR166, miRN88, and miRN74, along with their target genes annotated as F-box/kelch-repeat protein, 60S ribosomal protein, copper-zinc superoxide dismutase, luminal-binding protein, S-adenosylmethionine synthase, and Early Responsive to Dehydration Stress may play critical roles in drought response. This study provides insights into microRNA responsive to drought and rewatering in Masson pine and advances the understanding of drought tolerance mechanisms in Pinus. MDPI 2023-09-28 /pmc/articles/PMC10572226/ /pubmed/37834103 http://dx.doi.org/10.3390/ijms241914655 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Chen, Xinhua Chen, Hu Shen, Tengfei Luo, Qunfeng Xu, Meng Yang, Zhangqi The miRNA-mRNA Regulatory Modules of Pinus massoniana Lamb. in Response to Drought Stress |
title | The miRNA-mRNA Regulatory Modules of Pinus massoniana Lamb. in Response to Drought Stress |
title_full | The miRNA-mRNA Regulatory Modules of Pinus massoniana Lamb. in Response to Drought Stress |
title_fullStr | The miRNA-mRNA Regulatory Modules of Pinus massoniana Lamb. in Response to Drought Stress |
title_full_unstemmed | The miRNA-mRNA Regulatory Modules of Pinus massoniana Lamb. in Response to Drought Stress |
title_short | The miRNA-mRNA Regulatory Modules of Pinus massoniana Lamb. in Response to Drought Stress |
title_sort | mirna-mrna regulatory modules of pinus massoniana lamb. in response to drought stress |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10572226/ https://www.ncbi.nlm.nih.gov/pubmed/37834103 http://dx.doi.org/10.3390/ijms241914655 |
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