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Water Extract of Angelica dahurica Inhibits Osteoclast Differentiation and Bone Loss
Angelica dahurica radix has a long history of traditional use in China and Korea for treating headaches, cold-damp pain and skin diseases. Despite various pharmacological studies on A. dahurica, its impact on bones remains unclear. Hence, this study investigated the inhibitory effect of A. dahurica’...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10572401/ https://www.ncbi.nlm.nih.gov/pubmed/37834161 http://dx.doi.org/10.3390/ijms241914715 |
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author | Gu, Dong Ryun Yang, Hyun Kim, Seong Cheol Hwang, Youn-Hwan Ha, Hyunil |
author_facet | Gu, Dong Ryun Yang, Hyun Kim, Seong Cheol Hwang, Youn-Hwan Ha, Hyunil |
author_sort | Gu, Dong Ryun |
collection | PubMed |
description | Angelica dahurica radix has a long history of traditional use in China and Korea for treating headaches, cold-damp pain and skin diseases. Despite various pharmacological studies on A. dahurica, its impact on bones remains unclear. Hence, this study investigated the inhibitory effect of A. dahurica’s radix water extract (WEAD) on osteoclast differentiation. In vitro experiments showed that WEAD effectively suppresses osteoclast differentiation. Treatment of an osteoclast precursor with WEAD significantly suppressed the expression of nuclear factor of activated T-cells 1 (NFATc1), essential transcription factor for osteoclastogenesis, while increasing the expression of negative regulators, interferon regulatory factor 8 (Irf8) and v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (MafB). Consistent with the in vitro findings, the oral administration of WEAD (100 and 300 mg/kg/day) to mice subjected to surgical ovariectomy for a duration of six weeks alleviated bone loss, while also mitigating weight gain and liver fat accumulation. In addition, we also identified phytochemicals present in WEAD, known to regulate osteoclastogenesis and/or bone loss. These results suggest the potential use of WEAD for treating various bone disorders caused by excessive bone resorption. |
format | Online Article Text |
id | pubmed-10572401 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105724012023-10-14 Water Extract of Angelica dahurica Inhibits Osteoclast Differentiation and Bone Loss Gu, Dong Ryun Yang, Hyun Kim, Seong Cheol Hwang, Youn-Hwan Ha, Hyunil Int J Mol Sci Article Angelica dahurica radix has a long history of traditional use in China and Korea for treating headaches, cold-damp pain and skin diseases. Despite various pharmacological studies on A. dahurica, its impact on bones remains unclear. Hence, this study investigated the inhibitory effect of A. dahurica’s radix water extract (WEAD) on osteoclast differentiation. In vitro experiments showed that WEAD effectively suppresses osteoclast differentiation. Treatment of an osteoclast precursor with WEAD significantly suppressed the expression of nuclear factor of activated T-cells 1 (NFATc1), essential transcription factor for osteoclastogenesis, while increasing the expression of negative regulators, interferon regulatory factor 8 (Irf8) and v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (MafB). Consistent with the in vitro findings, the oral administration of WEAD (100 and 300 mg/kg/day) to mice subjected to surgical ovariectomy for a duration of six weeks alleviated bone loss, while also mitigating weight gain and liver fat accumulation. In addition, we also identified phytochemicals present in WEAD, known to regulate osteoclastogenesis and/or bone loss. These results suggest the potential use of WEAD for treating various bone disorders caused by excessive bone resorption. MDPI 2023-09-28 /pmc/articles/PMC10572401/ /pubmed/37834161 http://dx.doi.org/10.3390/ijms241914715 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Gu, Dong Ryun Yang, Hyun Kim, Seong Cheol Hwang, Youn-Hwan Ha, Hyunil Water Extract of Angelica dahurica Inhibits Osteoclast Differentiation and Bone Loss |
title | Water Extract of Angelica dahurica Inhibits Osteoclast Differentiation and Bone Loss |
title_full | Water Extract of Angelica dahurica Inhibits Osteoclast Differentiation and Bone Loss |
title_fullStr | Water Extract of Angelica dahurica Inhibits Osteoclast Differentiation and Bone Loss |
title_full_unstemmed | Water Extract of Angelica dahurica Inhibits Osteoclast Differentiation and Bone Loss |
title_short | Water Extract of Angelica dahurica Inhibits Osteoclast Differentiation and Bone Loss |
title_sort | water extract of angelica dahurica inhibits osteoclast differentiation and bone loss |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10572401/ https://www.ncbi.nlm.nih.gov/pubmed/37834161 http://dx.doi.org/10.3390/ijms241914715 |
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