Cargando…
Baicalin Attenuates Panton–Valentine Leukocidin (PVL)-Induced Cytoskeleton Rearrangement via Regulating the RhoA/ROCK/LIMK and PI3K/AKT/GSK-3β Pathways in Bovine Mammary Epithelial Cells
Pore-forming toxins (PFTs) exert physiological effects by rearrangement of the host cell cytoskeleton. Staphylococcus aureus-secreted PFTs play an important role in bovine mastitis. In the study, we examined the effects of recombinant Panton–Valentine leukocidin (rPVL) on cytoskeleton rearrangement,...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10572466/ https://www.ncbi.nlm.nih.gov/pubmed/37833969 http://dx.doi.org/10.3390/ijms241914520 |
_version_ | 1785120241918935040 |
---|---|
author | Yang, Jiangliu Hai, Zhenzhen Hou, Ling Liu, Yang Zhang, Dongtao Zhou, Xuezhang |
author_facet | Yang, Jiangliu Hai, Zhenzhen Hou, Ling Liu, Yang Zhang, Dongtao Zhou, Xuezhang |
author_sort | Yang, Jiangliu |
collection | PubMed |
description | Pore-forming toxins (PFTs) exert physiological effects by rearrangement of the host cell cytoskeleton. Staphylococcus aureus-secreted PFTs play an important role in bovine mastitis. In the study, we examined the effects of recombinant Panton–Valentine leukocidin (rPVL) on cytoskeleton rearrangement, and identified the signaling pathways involved in regulating the process in bovine mammary epithelial cells (BMECs) in vitro. Meanwhile, the underlying regulatory mechanism of baicalin for this process was investigated. The results showed that S. aureus induced cytoskeleton rearrangement in BMECs mainly through PVL. S. aureus and rPVL caused alterations in the cell morphology and layer integrity due to microfilament and microtubule rearrangement and focal contact inability. rPVL strongly induced the phosphorylation of cofilin at Ser3 mediating by the activation of the RhoA/ROCK/LIMK pathway, and resulted in the activation of loss of actin stress fibers, or the hyperphosphorylation of Tau at Ser396 inducing by the inhibition of the PI3K/AKT/GSK-3β pathways, and decreased the microtubule assembly. Baicalin significantly attenuated rPVL-stimulated cytoskeleton rearrangement in BMECs. Baicalin inhibited cofilin phosphorylation or Tau hyperphosphorylation via regulating the activation of RhoA/ROCK/LIMK and PI3K/AKT/GSK-3β signaling pathways. These findings provide new insights into the pathogenesis and potential treatment in S. aureus causing bovine mastitis. |
format | Online Article Text |
id | pubmed-10572466 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105724662023-10-14 Baicalin Attenuates Panton–Valentine Leukocidin (PVL)-Induced Cytoskeleton Rearrangement via Regulating the RhoA/ROCK/LIMK and PI3K/AKT/GSK-3β Pathways in Bovine Mammary Epithelial Cells Yang, Jiangliu Hai, Zhenzhen Hou, Ling Liu, Yang Zhang, Dongtao Zhou, Xuezhang Int J Mol Sci Article Pore-forming toxins (PFTs) exert physiological effects by rearrangement of the host cell cytoskeleton. Staphylococcus aureus-secreted PFTs play an important role in bovine mastitis. In the study, we examined the effects of recombinant Panton–Valentine leukocidin (rPVL) on cytoskeleton rearrangement, and identified the signaling pathways involved in regulating the process in bovine mammary epithelial cells (BMECs) in vitro. Meanwhile, the underlying regulatory mechanism of baicalin for this process was investigated. The results showed that S. aureus induced cytoskeleton rearrangement in BMECs mainly through PVL. S. aureus and rPVL caused alterations in the cell morphology and layer integrity due to microfilament and microtubule rearrangement and focal contact inability. rPVL strongly induced the phosphorylation of cofilin at Ser3 mediating by the activation of the RhoA/ROCK/LIMK pathway, and resulted in the activation of loss of actin stress fibers, or the hyperphosphorylation of Tau at Ser396 inducing by the inhibition of the PI3K/AKT/GSK-3β pathways, and decreased the microtubule assembly. Baicalin significantly attenuated rPVL-stimulated cytoskeleton rearrangement in BMECs. Baicalin inhibited cofilin phosphorylation or Tau hyperphosphorylation via regulating the activation of RhoA/ROCK/LIMK and PI3K/AKT/GSK-3β signaling pathways. These findings provide new insights into the pathogenesis and potential treatment in S. aureus causing bovine mastitis. MDPI 2023-09-25 /pmc/articles/PMC10572466/ /pubmed/37833969 http://dx.doi.org/10.3390/ijms241914520 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yang, Jiangliu Hai, Zhenzhen Hou, Ling Liu, Yang Zhang, Dongtao Zhou, Xuezhang Baicalin Attenuates Panton–Valentine Leukocidin (PVL)-Induced Cytoskeleton Rearrangement via Regulating the RhoA/ROCK/LIMK and PI3K/AKT/GSK-3β Pathways in Bovine Mammary Epithelial Cells |
title | Baicalin Attenuates Panton–Valentine Leukocidin (PVL)-Induced Cytoskeleton Rearrangement via Regulating the RhoA/ROCK/LIMK and PI3K/AKT/GSK-3β Pathways in Bovine Mammary Epithelial Cells |
title_full | Baicalin Attenuates Panton–Valentine Leukocidin (PVL)-Induced Cytoskeleton Rearrangement via Regulating the RhoA/ROCK/LIMK and PI3K/AKT/GSK-3β Pathways in Bovine Mammary Epithelial Cells |
title_fullStr | Baicalin Attenuates Panton–Valentine Leukocidin (PVL)-Induced Cytoskeleton Rearrangement via Regulating the RhoA/ROCK/LIMK and PI3K/AKT/GSK-3β Pathways in Bovine Mammary Epithelial Cells |
title_full_unstemmed | Baicalin Attenuates Panton–Valentine Leukocidin (PVL)-Induced Cytoskeleton Rearrangement via Regulating the RhoA/ROCK/LIMK and PI3K/AKT/GSK-3β Pathways in Bovine Mammary Epithelial Cells |
title_short | Baicalin Attenuates Panton–Valentine Leukocidin (PVL)-Induced Cytoskeleton Rearrangement via Regulating the RhoA/ROCK/LIMK and PI3K/AKT/GSK-3β Pathways in Bovine Mammary Epithelial Cells |
title_sort | baicalin attenuates panton–valentine leukocidin (pvl)-induced cytoskeleton rearrangement via regulating the rhoa/rock/limk and pi3k/akt/gsk-3β pathways in bovine mammary epithelial cells |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10572466/ https://www.ncbi.nlm.nih.gov/pubmed/37833969 http://dx.doi.org/10.3390/ijms241914520 |
work_keys_str_mv | AT yangjiangliu baicalinattenuatespantonvalentineleukocidinpvlinducedcytoskeletonrearrangementviaregulatingtherhoarocklimkandpi3kaktgsk3bpathwaysinbovinemammaryepithelialcells AT haizhenzhen baicalinattenuatespantonvalentineleukocidinpvlinducedcytoskeletonrearrangementviaregulatingtherhoarocklimkandpi3kaktgsk3bpathwaysinbovinemammaryepithelialcells AT houling baicalinattenuatespantonvalentineleukocidinpvlinducedcytoskeletonrearrangementviaregulatingtherhoarocklimkandpi3kaktgsk3bpathwaysinbovinemammaryepithelialcells AT liuyang baicalinattenuatespantonvalentineleukocidinpvlinducedcytoskeletonrearrangementviaregulatingtherhoarocklimkandpi3kaktgsk3bpathwaysinbovinemammaryepithelialcells AT zhangdongtao baicalinattenuatespantonvalentineleukocidinpvlinducedcytoskeletonrearrangementviaregulatingtherhoarocklimkandpi3kaktgsk3bpathwaysinbovinemammaryepithelialcells AT zhouxuezhang baicalinattenuatespantonvalentineleukocidinpvlinducedcytoskeletonrearrangementviaregulatingtherhoarocklimkandpi3kaktgsk3bpathwaysinbovinemammaryepithelialcells |