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The Study of the Caudal Vertebrae of Thick-Toed Geckos after a Prolonged Space Flight by X-ray Phase-Contrast Micro-CT

The proximal caudal vertebrae and notochord in thick-toed geckos (TG) (Chondrodactylus turneri, Gray, 1864) were investigated after a 30-day space flight onboard the biosatellite Bion-M1. This region has not been explored in previous studies. Our research focused on finding sites most affected by de...

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Autores principales: Bukreeva, Inna, Gulimova, Victoria I., Krivonosov, Yuri S., Buzmakov, Alexey V., Junemann, Olga, Cedola, Alessia, Fratini, Michela, Maugeri, Laura, Begani Provinciali, Ginevra, Palermo, Francesca, Sanna, Alessia, Pieroni, Nicola, Asadchikov, Victor E., Saveliev, Sergey V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10572532/
https://www.ncbi.nlm.nih.gov/pubmed/37830629
http://dx.doi.org/10.3390/cells12192415
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author Bukreeva, Inna
Gulimova, Victoria I.
Krivonosov, Yuri S.
Buzmakov, Alexey V.
Junemann, Olga
Cedola, Alessia
Fratini, Michela
Maugeri, Laura
Begani Provinciali, Ginevra
Palermo, Francesca
Sanna, Alessia
Pieroni, Nicola
Asadchikov, Victor E.
Saveliev, Sergey V.
author_facet Bukreeva, Inna
Gulimova, Victoria I.
Krivonosov, Yuri S.
Buzmakov, Alexey V.
Junemann, Olga
Cedola, Alessia
Fratini, Michela
Maugeri, Laura
Begani Provinciali, Ginevra
Palermo, Francesca
Sanna, Alessia
Pieroni, Nicola
Asadchikov, Victor E.
Saveliev, Sergey V.
author_sort Bukreeva, Inna
collection PubMed
description The proximal caudal vertebrae and notochord in thick-toed geckos (TG) (Chondrodactylus turneri, Gray, 1864) were investigated after a 30-day space flight onboard the biosatellite Bion-M1. This region has not been explored in previous studies. Our research focused on finding sites most affected by demineralization caused by microgravity (G0). We used X-ray phase-contrast tomography to study TG samples without invasive prior preparation to clarify our previous findings on the resistance of TG’s bones to demineralization in G0. The results of the present study confirmed that geckos are capable of preserving bone mass after flight, as neither cortical nor trabecular bone volume fraction showed statistically significant changes after flight. On the other hand, we observed a clear decrease in the mineralization of the notochordal septum and a substantial rise in intercentrum volume following the flight. To monitor TG’s mineral metabolism in G0, we propose to measure the volume of mineralized tissue in the notochordal septum. This technique holds promise as a sensitive approach to track the demineralization process in G0, given that the volume of calcification within the septum is limited, making it easy to detect even slight changes in mineral content.
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spelling pubmed-105725322023-10-14 The Study of the Caudal Vertebrae of Thick-Toed Geckos after a Prolonged Space Flight by X-ray Phase-Contrast Micro-CT Bukreeva, Inna Gulimova, Victoria I. Krivonosov, Yuri S. Buzmakov, Alexey V. Junemann, Olga Cedola, Alessia Fratini, Michela Maugeri, Laura Begani Provinciali, Ginevra Palermo, Francesca Sanna, Alessia Pieroni, Nicola Asadchikov, Victor E. Saveliev, Sergey V. Cells Article The proximal caudal vertebrae and notochord in thick-toed geckos (TG) (Chondrodactylus turneri, Gray, 1864) were investigated after a 30-day space flight onboard the biosatellite Bion-M1. This region has not been explored in previous studies. Our research focused on finding sites most affected by demineralization caused by microgravity (G0). We used X-ray phase-contrast tomography to study TG samples without invasive prior preparation to clarify our previous findings on the resistance of TG’s bones to demineralization in G0. The results of the present study confirmed that geckos are capable of preserving bone mass after flight, as neither cortical nor trabecular bone volume fraction showed statistically significant changes after flight. On the other hand, we observed a clear decrease in the mineralization of the notochordal septum and a substantial rise in intercentrum volume following the flight. To monitor TG’s mineral metabolism in G0, we propose to measure the volume of mineralized tissue in the notochordal septum. This technique holds promise as a sensitive approach to track the demineralization process in G0, given that the volume of calcification within the septum is limited, making it easy to detect even slight changes in mineral content. MDPI 2023-10-07 /pmc/articles/PMC10572532/ /pubmed/37830629 http://dx.doi.org/10.3390/cells12192415 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bukreeva, Inna
Gulimova, Victoria I.
Krivonosov, Yuri S.
Buzmakov, Alexey V.
Junemann, Olga
Cedola, Alessia
Fratini, Michela
Maugeri, Laura
Begani Provinciali, Ginevra
Palermo, Francesca
Sanna, Alessia
Pieroni, Nicola
Asadchikov, Victor E.
Saveliev, Sergey V.
The Study of the Caudal Vertebrae of Thick-Toed Geckos after a Prolonged Space Flight by X-ray Phase-Contrast Micro-CT
title The Study of the Caudal Vertebrae of Thick-Toed Geckos after a Prolonged Space Flight by X-ray Phase-Contrast Micro-CT
title_full The Study of the Caudal Vertebrae of Thick-Toed Geckos after a Prolonged Space Flight by X-ray Phase-Contrast Micro-CT
title_fullStr The Study of the Caudal Vertebrae of Thick-Toed Geckos after a Prolonged Space Flight by X-ray Phase-Contrast Micro-CT
title_full_unstemmed The Study of the Caudal Vertebrae of Thick-Toed Geckos after a Prolonged Space Flight by X-ray Phase-Contrast Micro-CT
title_short The Study of the Caudal Vertebrae of Thick-Toed Geckos after a Prolonged Space Flight by X-ray Phase-Contrast Micro-CT
title_sort study of the caudal vertebrae of thick-toed geckos after a prolonged space flight by x-ray phase-contrast micro-ct
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10572532/
https://www.ncbi.nlm.nih.gov/pubmed/37830629
http://dx.doi.org/10.3390/cells12192415
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