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Gene Expression Profiling Reveals Potential Players of Sex Determination and Asymmetrical Development in Chicken Embryo Gonads
Despite the notable progress made in recent years, the understanding of the genetic control of gonadal sex differentiation and asymmetrical ovariogenesis in chicken during embryonic development remains incomplete. This study aimed to identify potential key genes and speculate about the mechanisms as...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10572726/ https://www.ncbi.nlm.nih.gov/pubmed/37834055 http://dx.doi.org/10.3390/ijms241914597 |
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author | Luo, Huaixi Zhou, Hao Jiang, Shengyao He, Chuan Xu, Ke Ding, Jinmei Liu, Jiajia Qin, Chao Chen, Kangchun Zhou, Wenchuan Wang, Liyuan Yang, Wenhao Zhu, Wenqi Meng, He |
author_facet | Luo, Huaixi Zhou, Hao Jiang, Shengyao He, Chuan Xu, Ke Ding, Jinmei Liu, Jiajia Qin, Chao Chen, Kangchun Zhou, Wenchuan Wang, Liyuan Yang, Wenhao Zhu, Wenqi Meng, He |
author_sort | Luo, Huaixi |
collection | PubMed |
description | Despite the notable progress made in recent years, the understanding of the genetic control of gonadal sex differentiation and asymmetrical ovariogenesis in chicken during embryonic development remains incomplete. This study aimed to identify potential key genes and speculate about the mechanisms associated with ovary and testis development via an analysis of the results of PacBio and Illumina transcriptome sequencing of embryonic chicken gonads at the initiation of sexual differentiation (E4.5, E5.5, and E6.5). PacBio sequencing detected 328 and 233 significantly up-regulated transcript isoforms in females and males at E4.5, respectively. Illumina sequencing detected 95, 296 and 445 DEGs at E4.5, E5.5, and E6.5, respectively. Moreover, both sexes showed asymmetrical expression in gonads, and more DEGs were detected on the left side. There were 12 DEGs involved in cell proliferation shared between males and females in the left gonads. GO analysis suggested that coagulation pathways may be involved in the degradation of the right gonad in females and that blood oxygen transport pathways may be involved in preventing the degradation of the right gonad in males. These results provide a comprehensive expression profile of chicken embryo gonads at the initiation of sexual differentiation, which can serve as a theoretical basis for further understanding the mechanism of bird sex determination and its evolutionary process. |
format | Online Article Text |
id | pubmed-10572726 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105727262023-10-14 Gene Expression Profiling Reveals Potential Players of Sex Determination and Asymmetrical Development in Chicken Embryo Gonads Luo, Huaixi Zhou, Hao Jiang, Shengyao He, Chuan Xu, Ke Ding, Jinmei Liu, Jiajia Qin, Chao Chen, Kangchun Zhou, Wenchuan Wang, Liyuan Yang, Wenhao Zhu, Wenqi Meng, He Int J Mol Sci Article Despite the notable progress made in recent years, the understanding of the genetic control of gonadal sex differentiation and asymmetrical ovariogenesis in chicken during embryonic development remains incomplete. This study aimed to identify potential key genes and speculate about the mechanisms associated with ovary and testis development via an analysis of the results of PacBio and Illumina transcriptome sequencing of embryonic chicken gonads at the initiation of sexual differentiation (E4.5, E5.5, and E6.5). PacBio sequencing detected 328 and 233 significantly up-regulated transcript isoforms in females and males at E4.5, respectively. Illumina sequencing detected 95, 296 and 445 DEGs at E4.5, E5.5, and E6.5, respectively. Moreover, both sexes showed asymmetrical expression in gonads, and more DEGs were detected on the left side. There were 12 DEGs involved in cell proliferation shared between males and females in the left gonads. GO analysis suggested that coagulation pathways may be involved in the degradation of the right gonad in females and that blood oxygen transport pathways may be involved in preventing the degradation of the right gonad in males. These results provide a comprehensive expression profile of chicken embryo gonads at the initiation of sexual differentiation, which can serve as a theoretical basis for further understanding the mechanism of bird sex determination and its evolutionary process. MDPI 2023-09-27 /pmc/articles/PMC10572726/ /pubmed/37834055 http://dx.doi.org/10.3390/ijms241914597 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Luo, Huaixi Zhou, Hao Jiang, Shengyao He, Chuan Xu, Ke Ding, Jinmei Liu, Jiajia Qin, Chao Chen, Kangchun Zhou, Wenchuan Wang, Liyuan Yang, Wenhao Zhu, Wenqi Meng, He Gene Expression Profiling Reveals Potential Players of Sex Determination and Asymmetrical Development in Chicken Embryo Gonads |
title | Gene Expression Profiling Reveals Potential Players of Sex Determination and Asymmetrical Development in Chicken Embryo Gonads |
title_full | Gene Expression Profiling Reveals Potential Players of Sex Determination and Asymmetrical Development in Chicken Embryo Gonads |
title_fullStr | Gene Expression Profiling Reveals Potential Players of Sex Determination and Asymmetrical Development in Chicken Embryo Gonads |
title_full_unstemmed | Gene Expression Profiling Reveals Potential Players of Sex Determination and Asymmetrical Development in Chicken Embryo Gonads |
title_short | Gene Expression Profiling Reveals Potential Players of Sex Determination and Asymmetrical Development in Chicken Embryo Gonads |
title_sort | gene expression profiling reveals potential players of sex determination and asymmetrical development in chicken embryo gonads |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10572726/ https://www.ncbi.nlm.nih.gov/pubmed/37834055 http://dx.doi.org/10.3390/ijms241914597 |
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