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Plant Cell-Engineered Gold Nanoparticles Conjugated to Quercetin Inhibit SARS-CoV-2 and HSV-1 Entry

Recent studies have revealed considerable promise in the antiviral properties of metal nanomaterials, specifically when biologically prepared. This study demonstrates for the first time the antiviral roles of the plant cell-engineered gold nanoparticles (pAuNPs) alone and when conjugated with querce...

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Autores principales: Elste, James, Kumari, Sangeeta, Sharma, Nilesh, Razo, Erendira Palomino, Azhar, Eisa, Gao, Feng, Nunez, Maria Cuevas, Anwar, Wasim, Mitchell, John C., Tiwari, Vaibhav, Sahi, Shivendra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10573121/
https://www.ncbi.nlm.nih.gov/pubmed/37834240
http://dx.doi.org/10.3390/ijms241914792
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author Elste, James
Kumari, Sangeeta
Sharma, Nilesh
Razo, Erendira Palomino
Azhar, Eisa
Gao, Feng
Nunez, Maria Cuevas
Anwar, Wasim
Mitchell, John C.
Tiwari, Vaibhav
Sahi, Shivendra
author_facet Elste, James
Kumari, Sangeeta
Sharma, Nilesh
Razo, Erendira Palomino
Azhar, Eisa
Gao, Feng
Nunez, Maria Cuevas
Anwar, Wasim
Mitchell, John C.
Tiwari, Vaibhav
Sahi, Shivendra
author_sort Elste, James
collection PubMed
description Recent studies have revealed considerable promise in the antiviral properties of metal nanomaterials, specifically when biologically prepared. This study demonstrates for the first time the antiviral roles of the plant cell-engineered gold nanoparticles (pAuNPs) alone and when conjugated with quercetin (pAuNPsQ). We show here that the quercetin conjugated nanoparticles (pAuNPsQ) preferentially inhibit the cell entry of two medically important viruses—severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and herpes simplex virus type-1 (HSV-1) using different mechanisms. Interestingly, in the case of SARS-CoV-2, the pre-treatment of target cells with pAuNPsQ inhibited the viral entry, but the pre-treatment of the virus with pAuNPsQ did not affect viral entry into the host cell. In contrast, pAuNPsQ demonstrated effective blocking capabilities against HSV-1 entry, either during the pre-treatment of target cells or by inducing virus neutralization. In addition, pAuNPsQ also significantly affected HSV-1 replication, evidenced by the plaque-counting assay. In this study, we also tested the chemically synthesized gold nanoparticles (cAuNPs) of identical size and shape and observed comparable effects. The versatility of plant cell-based nanomaterial fabrication and its modification with bioactive compounds opens a new frontier in therapeutics, specifically in designing novel antiviral formulations.
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spelling pubmed-105731212023-10-14 Plant Cell-Engineered Gold Nanoparticles Conjugated to Quercetin Inhibit SARS-CoV-2 and HSV-1 Entry Elste, James Kumari, Sangeeta Sharma, Nilesh Razo, Erendira Palomino Azhar, Eisa Gao, Feng Nunez, Maria Cuevas Anwar, Wasim Mitchell, John C. Tiwari, Vaibhav Sahi, Shivendra Int J Mol Sci Article Recent studies have revealed considerable promise in the antiviral properties of metal nanomaterials, specifically when biologically prepared. This study demonstrates for the first time the antiviral roles of the plant cell-engineered gold nanoparticles (pAuNPs) alone and when conjugated with quercetin (pAuNPsQ). We show here that the quercetin conjugated nanoparticles (pAuNPsQ) preferentially inhibit the cell entry of two medically important viruses—severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and herpes simplex virus type-1 (HSV-1) using different mechanisms. Interestingly, in the case of SARS-CoV-2, the pre-treatment of target cells with pAuNPsQ inhibited the viral entry, but the pre-treatment of the virus with pAuNPsQ did not affect viral entry into the host cell. In contrast, pAuNPsQ demonstrated effective blocking capabilities against HSV-1 entry, either during the pre-treatment of target cells or by inducing virus neutralization. In addition, pAuNPsQ also significantly affected HSV-1 replication, evidenced by the plaque-counting assay. In this study, we also tested the chemically synthesized gold nanoparticles (cAuNPs) of identical size and shape and observed comparable effects. The versatility of plant cell-based nanomaterial fabrication and its modification with bioactive compounds opens a new frontier in therapeutics, specifically in designing novel antiviral formulations. MDPI 2023-09-30 /pmc/articles/PMC10573121/ /pubmed/37834240 http://dx.doi.org/10.3390/ijms241914792 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Elste, James
Kumari, Sangeeta
Sharma, Nilesh
Razo, Erendira Palomino
Azhar, Eisa
Gao, Feng
Nunez, Maria Cuevas
Anwar, Wasim
Mitchell, John C.
Tiwari, Vaibhav
Sahi, Shivendra
Plant Cell-Engineered Gold Nanoparticles Conjugated to Quercetin Inhibit SARS-CoV-2 and HSV-1 Entry
title Plant Cell-Engineered Gold Nanoparticles Conjugated to Quercetin Inhibit SARS-CoV-2 and HSV-1 Entry
title_full Plant Cell-Engineered Gold Nanoparticles Conjugated to Quercetin Inhibit SARS-CoV-2 and HSV-1 Entry
title_fullStr Plant Cell-Engineered Gold Nanoparticles Conjugated to Quercetin Inhibit SARS-CoV-2 and HSV-1 Entry
title_full_unstemmed Plant Cell-Engineered Gold Nanoparticles Conjugated to Quercetin Inhibit SARS-CoV-2 and HSV-1 Entry
title_short Plant Cell-Engineered Gold Nanoparticles Conjugated to Quercetin Inhibit SARS-CoV-2 and HSV-1 Entry
title_sort plant cell-engineered gold nanoparticles conjugated to quercetin inhibit sars-cov-2 and hsv-1 entry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10573121/
https://www.ncbi.nlm.nih.gov/pubmed/37834240
http://dx.doi.org/10.3390/ijms241914792
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