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Multi-Omics Profiling of Human Endothelial Cells from the Coronary Artery and Internal Thoracic Artery Reveals Molecular but Not Functional Heterogeneity

Major adverse cardiovascular events occurring upon coronary artery bypass graft surgery are typically accompanied by endothelial dysfunction. Total arterial revascularisation, which employs both left and right internal thoracic arteries instead of the saphenous vein to create a bypass, is associated...

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Autores principales: Frolov, Alexey, Lobov, Arseniy, Kabilov, Marsel, Zainullina, Bozhana, Tupikin, Alexey, Shishkova, Daria, Markova, Victoria, Sinitskaya, Anna, Grigoriev, Evgeny, Markova, Yulia, Kutikhin, Anton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10573276/
https://www.ncbi.nlm.nih.gov/pubmed/37834480
http://dx.doi.org/10.3390/ijms241915032
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author Frolov, Alexey
Lobov, Arseniy
Kabilov, Marsel
Zainullina, Bozhana
Tupikin, Alexey
Shishkova, Daria
Markova, Victoria
Sinitskaya, Anna
Grigoriev, Evgeny
Markova, Yulia
Kutikhin, Anton
author_facet Frolov, Alexey
Lobov, Arseniy
Kabilov, Marsel
Zainullina, Bozhana
Tupikin, Alexey
Shishkova, Daria
Markova, Victoria
Sinitskaya, Anna
Grigoriev, Evgeny
Markova, Yulia
Kutikhin, Anton
author_sort Frolov, Alexey
collection PubMed
description Major adverse cardiovascular events occurring upon coronary artery bypass graft surgery are typically accompanied by endothelial dysfunction. Total arterial revascularisation, which employs both left and right internal thoracic arteries instead of the saphenous vein to create a bypass, is associated with better mid- and long-term outcomes. We suggested that molecular profiles of human coronary artery endothelial cells (HCAECs) and human internal mammary artery endothelial cells (HITAECs) are coherent in terms of transcriptomic and proteomic signatures, which were then investigated by RNA sequencing and ultra-high performance liquid chromatography-mass spectrometry, respectively. Both HCAECs and HITAECs overexpressed molecules responsible for the synthesis of extracellular matrix (ECM) components, basement membrane assembly, cell-ECM adhesion, organisation of intercellular junctions, and secretion of extracellular vesicles. HCAECs were characterised by higher enrichment with molecular signatures of basement membrane construction, collagen biosynthesis and folding, and formation of intercellular junctions, whilst HITAECs were notable for augmented pro-inflammatory signaling, intensive synthesis of proteins and nitrogen compounds, and enhanced ribosome biogenesis. Despite HCAECs and HITAECs showing a certain degree of molecular heterogeneity, no specific markers at the protein level have been identified. Coherence of differentially expressed molecular categories in HCAECs and HITAECs suggests synergistic interactions between these ECs in a bypass surgery scenario.
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spelling pubmed-105732762023-10-14 Multi-Omics Profiling of Human Endothelial Cells from the Coronary Artery and Internal Thoracic Artery Reveals Molecular but Not Functional Heterogeneity Frolov, Alexey Lobov, Arseniy Kabilov, Marsel Zainullina, Bozhana Tupikin, Alexey Shishkova, Daria Markova, Victoria Sinitskaya, Anna Grigoriev, Evgeny Markova, Yulia Kutikhin, Anton Int J Mol Sci Article Major adverse cardiovascular events occurring upon coronary artery bypass graft surgery are typically accompanied by endothelial dysfunction. Total arterial revascularisation, which employs both left and right internal thoracic arteries instead of the saphenous vein to create a bypass, is associated with better mid- and long-term outcomes. We suggested that molecular profiles of human coronary artery endothelial cells (HCAECs) and human internal mammary artery endothelial cells (HITAECs) are coherent in terms of transcriptomic and proteomic signatures, which were then investigated by RNA sequencing and ultra-high performance liquid chromatography-mass spectrometry, respectively. Both HCAECs and HITAECs overexpressed molecules responsible for the synthesis of extracellular matrix (ECM) components, basement membrane assembly, cell-ECM adhesion, organisation of intercellular junctions, and secretion of extracellular vesicles. HCAECs were characterised by higher enrichment with molecular signatures of basement membrane construction, collagen biosynthesis and folding, and formation of intercellular junctions, whilst HITAECs were notable for augmented pro-inflammatory signaling, intensive synthesis of proteins and nitrogen compounds, and enhanced ribosome biogenesis. Despite HCAECs and HITAECs showing a certain degree of molecular heterogeneity, no specific markers at the protein level have been identified. Coherence of differentially expressed molecular categories in HCAECs and HITAECs suggests synergistic interactions between these ECs in a bypass surgery scenario. MDPI 2023-10-09 /pmc/articles/PMC10573276/ /pubmed/37834480 http://dx.doi.org/10.3390/ijms241915032 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Frolov, Alexey
Lobov, Arseniy
Kabilov, Marsel
Zainullina, Bozhana
Tupikin, Alexey
Shishkova, Daria
Markova, Victoria
Sinitskaya, Anna
Grigoriev, Evgeny
Markova, Yulia
Kutikhin, Anton
Multi-Omics Profiling of Human Endothelial Cells from the Coronary Artery and Internal Thoracic Artery Reveals Molecular but Not Functional Heterogeneity
title Multi-Omics Profiling of Human Endothelial Cells from the Coronary Artery and Internal Thoracic Artery Reveals Molecular but Not Functional Heterogeneity
title_full Multi-Omics Profiling of Human Endothelial Cells from the Coronary Artery and Internal Thoracic Artery Reveals Molecular but Not Functional Heterogeneity
title_fullStr Multi-Omics Profiling of Human Endothelial Cells from the Coronary Artery and Internal Thoracic Artery Reveals Molecular but Not Functional Heterogeneity
title_full_unstemmed Multi-Omics Profiling of Human Endothelial Cells from the Coronary Artery and Internal Thoracic Artery Reveals Molecular but Not Functional Heterogeneity
title_short Multi-Omics Profiling of Human Endothelial Cells from the Coronary Artery and Internal Thoracic Artery Reveals Molecular but Not Functional Heterogeneity
title_sort multi-omics profiling of human endothelial cells from the coronary artery and internal thoracic artery reveals molecular but not functional heterogeneity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10573276/
https://www.ncbi.nlm.nih.gov/pubmed/37834480
http://dx.doi.org/10.3390/ijms241915032
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