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Two polysaccharides from Rehmannia glutinosa: isolation, structural characterization, and hypoglycemic activities
Rehmannia glutinosa (RG) as a Chinese herbal medicine can be used both in medicine and food. As the main component of RG, the polysaccharides have a hypoglycemic effect, however, the hypoglycemic activity of RG homopolysaccharides remains unknown. We isolated and purified two polysaccharides, RGP70-...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Royal Society of Chemistry
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10573874/ https://www.ncbi.nlm.nih.gov/pubmed/37842674 http://dx.doi.org/10.1039/d3ra05677e |
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author | Chen, Huien Liu, Xinyu Xie, Meixia Zhong, Xiaoting Yan, Chunyan Xian, Minghua Wang, Shumei |
author_facet | Chen, Huien Liu, Xinyu Xie, Meixia Zhong, Xiaoting Yan, Chunyan Xian, Minghua Wang, Shumei |
author_sort | Chen, Huien |
collection | PubMed |
description | Rehmannia glutinosa (RG) as a Chinese herbal medicine can be used both in medicine and food. As the main component of RG, the polysaccharides have a hypoglycemic effect, however, the hypoglycemic activity of RG homopolysaccharides remains unknown. We isolated and purified two polysaccharides, RGP70-1-1 and RGP70-1-2 (4.9 kDa and 2.8 kDa) from RG. The structural characteristics, including monosaccharide composition, linkage, and configuration were analyzed by FT-IR, HPLC, GC-MS, NMR spectroscopy, Congo test, and SEM. RGP70-1-1 and RGP70-1-2 consist of four monosaccharides (glucose, mannose, arabinose, and galactose). RGP70-1-1 contains 14 connection modes, with the linkages including l-Araf-(1→, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, d-Manp-(1→, →2)-d-Manp-(1→, →4)-d-Manp-(1→, d-Galp-(1→, →4)-d-Galp-(1→, →4,6)-d-Galp-(1→, →6)-d-Glcp-(1→, →4,6)-d-Glcp-(1→, →3,6)-d-Glcp-(1→. The linkages of RGP70-1-2 is including →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →4)-d-Manp-(1→, →3,6)-d-Manp-(1→, d-Galp-(1→, →6)-d-Galp-(1→, d-Glcp-(1→, →6)-d-Glcp-(1→, →4,6)-d-Glcp-(1→. Furthermore, RGP70-1-1 and RGP70-1-2 can inhibit α-glucosidase and α-amylase. RGP70-1-1 stimulated GLP-1 secretion in STC-1 cells and was related to the up-regulation of PI3K and p-AKT protein expression. The findings revealed a natural product with potential hypoglycemic activity, which may be used as a GLP-1 secretagogue and a beneficial functional food ingredient for T2D. |
format | Online Article Text |
id | pubmed-10573874 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Royal Society of Chemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-105738742023-10-14 Two polysaccharides from Rehmannia glutinosa: isolation, structural characterization, and hypoglycemic activities Chen, Huien Liu, Xinyu Xie, Meixia Zhong, Xiaoting Yan, Chunyan Xian, Minghua Wang, Shumei RSC Adv Chemistry Rehmannia glutinosa (RG) as a Chinese herbal medicine can be used both in medicine and food. As the main component of RG, the polysaccharides have a hypoglycemic effect, however, the hypoglycemic activity of RG homopolysaccharides remains unknown. We isolated and purified two polysaccharides, RGP70-1-1 and RGP70-1-2 (4.9 kDa and 2.8 kDa) from RG. The structural characteristics, including monosaccharide composition, linkage, and configuration were analyzed by FT-IR, HPLC, GC-MS, NMR spectroscopy, Congo test, and SEM. RGP70-1-1 and RGP70-1-2 consist of four monosaccharides (glucose, mannose, arabinose, and galactose). RGP70-1-1 contains 14 connection modes, with the linkages including l-Araf-(1→, →3)-l-Araf-(1→, →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →2,5)-l-Araf-(1→, d-Manp-(1→, →2)-d-Manp-(1→, →4)-d-Manp-(1→, d-Galp-(1→, →4)-d-Galp-(1→, →4,6)-d-Galp-(1→, →6)-d-Glcp-(1→, →4,6)-d-Glcp-(1→, →3,6)-d-Glcp-(1→. The linkages of RGP70-1-2 is including →5)-l-Araf-(1→, →3,5)-l-Araf-(1→, →4)-d-Manp-(1→, →3,6)-d-Manp-(1→, d-Galp-(1→, →6)-d-Galp-(1→, d-Glcp-(1→, →6)-d-Glcp-(1→, →4,6)-d-Glcp-(1→. Furthermore, RGP70-1-1 and RGP70-1-2 can inhibit α-glucosidase and α-amylase. RGP70-1-1 stimulated GLP-1 secretion in STC-1 cells and was related to the up-regulation of PI3K and p-AKT protein expression. The findings revealed a natural product with potential hypoglycemic activity, which may be used as a GLP-1 secretagogue and a beneficial functional food ingredient for T2D. The Royal Society of Chemistry 2023-10-13 /pmc/articles/PMC10573874/ /pubmed/37842674 http://dx.doi.org/10.1039/d3ra05677e Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Chen, Huien Liu, Xinyu Xie, Meixia Zhong, Xiaoting Yan, Chunyan Xian, Minghua Wang, Shumei Two polysaccharides from Rehmannia glutinosa: isolation, structural characterization, and hypoglycemic activities |
title | Two polysaccharides from Rehmannia glutinosa: isolation, structural characterization, and hypoglycemic activities |
title_full | Two polysaccharides from Rehmannia glutinosa: isolation, structural characterization, and hypoglycemic activities |
title_fullStr | Two polysaccharides from Rehmannia glutinosa: isolation, structural characterization, and hypoglycemic activities |
title_full_unstemmed | Two polysaccharides from Rehmannia glutinosa: isolation, structural characterization, and hypoglycemic activities |
title_short | Two polysaccharides from Rehmannia glutinosa: isolation, structural characterization, and hypoglycemic activities |
title_sort | two polysaccharides from rehmannia glutinosa: isolation, structural characterization, and hypoglycemic activities |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10573874/ https://www.ncbi.nlm.nih.gov/pubmed/37842674 http://dx.doi.org/10.1039/d3ra05677e |
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