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Effect of Cannabidiol on Human Peripheral Blood Mononuclear Cells and CD4+ T Cells

Cannabidiol (CBD), the main non-psychoactive component of Cannabis sativa L., is widely used in therapy for the treatment of different diseases and as an adjuvant drug. Our aim was to assess the effects of CBD on proinflammatory cytokine production and cell proliferation in human peripheral blood mo...

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Autores principales: Furgiuele, Alessia, Marino, Franca, Rasini, Emanuela, Legnaro, Massimiliano, Luini, Alessandra, Albizzati, Maria Giulia, di Flora, Alessia, Pacchetti, Barbara, Cosentino, Marco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10573927/
https://www.ncbi.nlm.nih.gov/pubmed/37834328
http://dx.doi.org/10.3390/ijms241914880
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author Furgiuele, Alessia
Marino, Franca
Rasini, Emanuela
Legnaro, Massimiliano
Luini, Alessandra
Albizzati, Maria Giulia
di Flora, Alessia
Pacchetti, Barbara
Cosentino, Marco
author_facet Furgiuele, Alessia
Marino, Franca
Rasini, Emanuela
Legnaro, Massimiliano
Luini, Alessandra
Albizzati, Maria Giulia
di Flora, Alessia
Pacchetti, Barbara
Cosentino, Marco
author_sort Furgiuele, Alessia
collection PubMed
description Cannabidiol (CBD), the main non-psychoactive component of Cannabis sativa L., is widely used in therapy for the treatment of different diseases and as an adjuvant drug. Our aim was to assess the effects of CBD on proinflammatory cytokine production and cell proliferation in human peripheral blood mononuclear cells (PBMCs) and on CD4+ T lymphocyte differentiation, and, furthermore, to test CBD’s ability to affect the functional properties of regulatory T cells (Treg). Experiments were performed on isolated PBMCs and purified CD4+ T lymphocytes obtained from the buffy coats of healthy subjects. Cytokines produced by CD4+ T cells were evaluated by flow cytometry and intracellular cytokine staining techniques. PBMC cytokine production was measured by an ELISA assay. Real-time PCR was used to assess the mRNA expression of cytokines and the key transcription factors (TFs) of CD4+ T cells. Finally, the proliferation of PBMC and CD4+ T effector cells (Teff), alone and in the presence of Treg, was assessed by flow cytometry. Results showed that CBD affects both the frequency of IL-4-producing CD4+ and of IFN-γ/IL-17-producing cells and dramatically decreases the mRNA levels of all TFs. Stimuli-induced cytokine mRNA expression was decreased while protein production was unaffected. CBD was unable to affect the ability of Treg to prevent Teff cell proliferation while it slightly increased PBMC proliferation. In conclusion, CBD may inhibit the expression of proinflammatory cytokines; however, the effect of CBD on cell proliferation suggests that this cannabinoid exerts a complex activity on human PBMCs and CD4+ T cells which deserves further investigation.
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spelling pubmed-105739272023-10-14 Effect of Cannabidiol on Human Peripheral Blood Mononuclear Cells and CD4+ T Cells Furgiuele, Alessia Marino, Franca Rasini, Emanuela Legnaro, Massimiliano Luini, Alessandra Albizzati, Maria Giulia di Flora, Alessia Pacchetti, Barbara Cosentino, Marco Int J Mol Sci Article Cannabidiol (CBD), the main non-psychoactive component of Cannabis sativa L., is widely used in therapy for the treatment of different diseases and as an adjuvant drug. Our aim was to assess the effects of CBD on proinflammatory cytokine production and cell proliferation in human peripheral blood mononuclear cells (PBMCs) and on CD4+ T lymphocyte differentiation, and, furthermore, to test CBD’s ability to affect the functional properties of regulatory T cells (Treg). Experiments were performed on isolated PBMCs and purified CD4+ T lymphocytes obtained from the buffy coats of healthy subjects. Cytokines produced by CD4+ T cells were evaluated by flow cytometry and intracellular cytokine staining techniques. PBMC cytokine production was measured by an ELISA assay. Real-time PCR was used to assess the mRNA expression of cytokines and the key transcription factors (TFs) of CD4+ T cells. Finally, the proliferation of PBMC and CD4+ T effector cells (Teff), alone and in the presence of Treg, was assessed by flow cytometry. Results showed that CBD affects both the frequency of IL-4-producing CD4+ and of IFN-γ/IL-17-producing cells and dramatically decreases the mRNA levels of all TFs. Stimuli-induced cytokine mRNA expression was decreased while protein production was unaffected. CBD was unable to affect the ability of Treg to prevent Teff cell proliferation while it slightly increased PBMC proliferation. In conclusion, CBD may inhibit the expression of proinflammatory cytokines; however, the effect of CBD on cell proliferation suggests that this cannabinoid exerts a complex activity on human PBMCs and CD4+ T cells which deserves further investigation. MDPI 2023-10-04 /pmc/articles/PMC10573927/ /pubmed/37834328 http://dx.doi.org/10.3390/ijms241914880 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Furgiuele, Alessia
Marino, Franca
Rasini, Emanuela
Legnaro, Massimiliano
Luini, Alessandra
Albizzati, Maria Giulia
di Flora, Alessia
Pacchetti, Barbara
Cosentino, Marco
Effect of Cannabidiol on Human Peripheral Blood Mononuclear Cells and CD4+ T Cells
title Effect of Cannabidiol on Human Peripheral Blood Mononuclear Cells and CD4+ T Cells
title_full Effect of Cannabidiol on Human Peripheral Blood Mononuclear Cells and CD4+ T Cells
title_fullStr Effect of Cannabidiol on Human Peripheral Blood Mononuclear Cells and CD4+ T Cells
title_full_unstemmed Effect of Cannabidiol on Human Peripheral Blood Mononuclear Cells and CD4+ T Cells
title_short Effect of Cannabidiol on Human Peripheral Blood Mononuclear Cells and CD4+ T Cells
title_sort effect of cannabidiol on human peripheral blood mononuclear cells and cd4+ t cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10573927/
https://www.ncbi.nlm.nih.gov/pubmed/37834328
http://dx.doi.org/10.3390/ijms241914880
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