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Xanthomonas euvesicatoria-Specific Bacteriophage BsXeu269p/3 Reduces the Spread of Bacterial Spot Disease in Pepper Plants

The present study was focused on the pathosystem pepper plants (Capsicum annuum L.)-phytopathogenic bacterium X. euvesicatoria (wild strain 269p)-bacteriophage BsXeu269p/3 and the possibility of bacteriophage-mediated biocontrol of the disease. Two new model systems were designed for the monitoring...

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Autores principales: Shopova, Elena, Brankova, Liliana, Ivanov, Sergei, Urshev, Zoltan, Dimitrova, Lyudmila, Dimitrova, Melani, Hristova, Petya, Kizheva, Yoana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574073/
https://www.ncbi.nlm.nih.gov/pubmed/37836088
http://dx.doi.org/10.3390/plants12193348
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author Shopova, Elena
Brankova, Liliana
Ivanov, Sergei
Urshev, Zoltan
Dimitrova, Lyudmila
Dimitrova, Melani
Hristova, Petya
Kizheva, Yoana
author_facet Shopova, Elena
Brankova, Liliana
Ivanov, Sergei
Urshev, Zoltan
Dimitrova, Lyudmila
Dimitrova, Melani
Hristova, Petya
Kizheva, Yoana
author_sort Shopova, Elena
collection PubMed
description The present study was focused on the pathosystem pepper plants (Capsicum annuum L.)-phytopathogenic bacterium X. euvesicatoria (wild strain 269p)-bacteriophage BsXeu269p/3 and the possibility of bacteriophage-mediated biocontrol of the disease. Two new model systems were designed for the monitoring of the effect of the phage treatment on the infectious process in vivo. The spread of the bacteriophage and the pathogen was monitored by qPCR. A new pair of primers for phage detection via qPCR was designed, as well as probes for TaqMan qPCR. The epiphytic bacterial population and the potential bacteriolytic effect of BsXeu269p/3 in vivo was observed by SEM. An aerosol-mediated transmission model system demonstrated that treatment with BsXeu269p/3 reduced the amount of X. euvesicatoria on the leaf surface five-fold. The needle-pricking model system showed a significant reduction of the amount of the pathogen in infectious lesions treated with BsXeu269p/3 (av. 59.7%), compared to the untreated control. We found that the phage titer is 10-fold higher in the infection lesions but it was still discoverable even in the absence of the specific host in the leaves. This is the first report of in vivo assessment of the biocontrol potential of locally isolated phages against BS pathogen X. euvesicatoria in Bulgaria.
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spelling pubmed-105740732023-10-14 Xanthomonas euvesicatoria-Specific Bacteriophage BsXeu269p/3 Reduces the Spread of Bacterial Spot Disease in Pepper Plants Shopova, Elena Brankova, Liliana Ivanov, Sergei Urshev, Zoltan Dimitrova, Lyudmila Dimitrova, Melani Hristova, Petya Kizheva, Yoana Plants (Basel) Article The present study was focused on the pathosystem pepper plants (Capsicum annuum L.)-phytopathogenic bacterium X. euvesicatoria (wild strain 269p)-bacteriophage BsXeu269p/3 and the possibility of bacteriophage-mediated biocontrol of the disease. Two new model systems were designed for the monitoring of the effect of the phage treatment on the infectious process in vivo. The spread of the bacteriophage and the pathogen was monitored by qPCR. A new pair of primers for phage detection via qPCR was designed, as well as probes for TaqMan qPCR. The epiphytic bacterial population and the potential bacteriolytic effect of BsXeu269p/3 in vivo was observed by SEM. An aerosol-mediated transmission model system demonstrated that treatment with BsXeu269p/3 reduced the amount of X. euvesicatoria on the leaf surface five-fold. The needle-pricking model system showed a significant reduction of the amount of the pathogen in infectious lesions treated with BsXeu269p/3 (av. 59.7%), compared to the untreated control. We found that the phage titer is 10-fold higher in the infection lesions but it was still discoverable even in the absence of the specific host in the leaves. This is the first report of in vivo assessment of the biocontrol potential of locally isolated phages against BS pathogen X. euvesicatoria in Bulgaria. MDPI 2023-09-22 /pmc/articles/PMC10574073/ /pubmed/37836088 http://dx.doi.org/10.3390/plants12193348 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shopova, Elena
Brankova, Liliana
Ivanov, Sergei
Urshev, Zoltan
Dimitrova, Lyudmila
Dimitrova, Melani
Hristova, Petya
Kizheva, Yoana
Xanthomonas euvesicatoria-Specific Bacteriophage BsXeu269p/3 Reduces the Spread of Bacterial Spot Disease in Pepper Plants
title Xanthomonas euvesicatoria-Specific Bacteriophage BsXeu269p/3 Reduces the Spread of Bacterial Spot Disease in Pepper Plants
title_full Xanthomonas euvesicatoria-Specific Bacteriophage BsXeu269p/3 Reduces the Spread of Bacterial Spot Disease in Pepper Plants
title_fullStr Xanthomonas euvesicatoria-Specific Bacteriophage BsXeu269p/3 Reduces the Spread of Bacterial Spot Disease in Pepper Plants
title_full_unstemmed Xanthomonas euvesicatoria-Specific Bacteriophage BsXeu269p/3 Reduces the Spread of Bacterial Spot Disease in Pepper Plants
title_short Xanthomonas euvesicatoria-Specific Bacteriophage BsXeu269p/3 Reduces the Spread of Bacterial Spot Disease in Pepper Plants
title_sort xanthomonas euvesicatoria-specific bacteriophage bsxeu269p/3 reduces the spread of bacterial spot disease in pepper plants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574073/
https://www.ncbi.nlm.nih.gov/pubmed/37836088
http://dx.doi.org/10.3390/plants12193348
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