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Identification and Expression Analysis of EPSPS and BAR Families in Cotton

Weeds seriously affect the yield and quality of crops. Because manual weeding is time-consuming and laborious, the use of herbicides becomes an effective way to solve the harm caused by weeds in fields. Both 5-enolpyruvyl shikimate-3-phosphate synthetase (EPSPS) and acetyltransferase genes (bialapho...

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Autores principales: Li, Zhao, Zhang, Zhen, Liu, Yinbo, Ma, Yuanqi, Lv, Xing, Zhang, Dongmei, Gu, Qishen, Ke, Huifeng, Wu, Liqiang, Zhang, Guiyin, Ma, Zhiying, Wang, Xingfen, Sun, Zhengwen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574212/
https://www.ncbi.nlm.nih.gov/pubmed/37836107
http://dx.doi.org/10.3390/plants12193366
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author Li, Zhao
Zhang, Zhen
Liu, Yinbo
Ma, Yuanqi
Lv, Xing
Zhang, Dongmei
Gu, Qishen
Ke, Huifeng
Wu, Liqiang
Zhang, Guiyin
Ma, Zhiying
Wang, Xingfen
Sun, Zhengwen
author_facet Li, Zhao
Zhang, Zhen
Liu, Yinbo
Ma, Yuanqi
Lv, Xing
Zhang, Dongmei
Gu, Qishen
Ke, Huifeng
Wu, Liqiang
Zhang, Guiyin
Ma, Zhiying
Wang, Xingfen
Sun, Zhengwen
author_sort Li, Zhao
collection PubMed
description Weeds seriously affect the yield and quality of crops. Because manual weeding is time-consuming and laborious, the use of herbicides becomes an effective way to solve the harm caused by weeds in fields. Both 5-enolpyruvyl shikimate-3-phosphate synthetase (EPSPS) and acetyltransferase genes (bialaphos resistance, BAR) are widely used to improve crop resistance to herbicides. However, cotton, as the most important natural fiber crop, is not tolerant to herbicides in China, and the EPSPS and BAR family genes have not yet been characterized in cotton. Therefore, we explore the genes of these two families to provide candidate genes for the study of herbicide resistance mechanisms. In this study, 8, 8, 4, and 5 EPSPS genes and 6, 6, 5, and 5 BAR genes were identified in allotetraploid Gossypium hirsutum and Gossypium barbadense, diploid Gossypium arboreum and Gossypium raimondii, respectively. Members of the EPSPS and BAR families were classified into three subgroups based on the distribution of phylogenetic trees, conserved motifs, and gene structures. In addition, the promoter sequences of EPSPS and BAR family members included growth and development, stress, and hormone-related cis-elements. Based on the expression analysis, the family members showed tissue-specific expression and differed significantly in response to abiotic stresses. Finally, qRT-PCR analysis revealed that the expression levels of GhEPSPS3, GhEPSPS4, and GhBAR1 were significantly upregulated after exogenous spraying of herbicides. Overall, we characterized the EPSPS and BAR gene families of cotton at the genome-wide level, which will provide a basis for further studying the functions of EPSPS and BAR genes during growth and development and herbicide stress.
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spelling pubmed-105742122023-10-14 Identification and Expression Analysis of EPSPS and BAR Families in Cotton Li, Zhao Zhang, Zhen Liu, Yinbo Ma, Yuanqi Lv, Xing Zhang, Dongmei Gu, Qishen Ke, Huifeng Wu, Liqiang Zhang, Guiyin Ma, Zhiying Wang, Xingfen Sun, Zhengwen Plants (Basel) Article Weeds seriously affect the yield and quality of crops. Because manual weeding is time-consuming and laborious, the use of herbicides becomes an effective way to solve the harm caused by weeds in fields. Both 5-enolpyruvyl shikimate-3-phosphate synthetase (EPSPS) and acetyltransferase genes (bialaphos resistance, BAR) are widely used to improve crop resistance to herbicides. However, cotton, as the most important natural fiber crop, is not tolerant to herbicides in China, and the EPSPS and BAR family genes have not yet been characterized in cotton. Therefore, we explore the genes of these two families to provide candidate genes for the study of herbicide resistance mechanisms. In this study, 8, 8, 4, and 5 EPSPS genes and 6, 6, 5, and 5 BAR genes were identified in allotetraploid Gossypium hirsutum and Gossypium barbadense, diploid Gossypium arboreum and Gossypium raimondii, respectively. Members of the EPSPS and BAR families were classified into three subgroups based on the distribution of phylogenetic trees, conserved motifs, and gene structures. In addition, the promoter sequences of EPSPS and BAR family members included growth and development, stress, and hormone-related cis-elements. Based on the expression analysis, the family members showed tissue-specific expression and differed significantly in response to abiotic stresses. Finally, qRT-PCR analysis revealed that the expression levels of GhEPSPS3, GhEPSPS4, and GhBAR1 were significantly upregulated after exogenous spraying of herbicides. Overall, we characterized the EPSPS and BAR gene families of cotton at the genome-wide level, which will provide a basis for further studying the functions of EPSPS and BAR genes during growth and development and herbicide stress. MDPI 2023-09-23 /pmc/articles/PMC10574212/ /pubmed/37836107 http://dx.doi.org/10.3390/plants12193366 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Zhao
Zhang, Zhen
Liu, Yinbo
Ma, Yuanqi
Lv, Xing
Zhang, Dongmei
Gu, Qishen
Ke, Huifeng
Wu, Liqiang
Zhang, Guiyin
Ma, Zhiying
Wang, Xingfen
Sun, Zhengwen
Identification and Expression Analysis of EPSPS and BAR Families in Cotton
title Identification and Expression Analysis of EPSPS and BAR Families in Cotton
title_full Identification and Expression Analysis of EPSPS and BAR Families in Cotton
title_fullStr Identification and Expression Analysis of EPSPS and BAR Families in Cotton
title_full_unstemmed Identification and Expression Analysis of EPSPS and BAR Families in Cotton
title_short Identification and Expression Analysis of EPSPS and BAR Families in Cotton
title_sort identification and expression analysis of epsps and bar families in cotton
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574212/
https://www.ncbi.nlm.nih.gov/pubmed/37836107
http://dx.doi.org/10.3390/plants12193366
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