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Metal-Promoted Higher-Order Assembly of Disulfide-Stapled Helical Barrels
Peptide-based helical barrels are a noteworthy building block for hierarchical assembly, with a hydrophobic cavity that can serve as a host for cargo. In this study, disulfide-stapled helical barrels were synthesized containing ligands for metal ions on the hydrophilic face of each amphiphilic pepti...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574645/ https://www.ncbi.nlm.nih.gov/pubmed/37836285 http://dx.doi.org/10.3390/nano13192645 |
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author | Agrahari, Ashutosh Lipton, Mark Chmielewski, Jean |
author_facet | Agrahari, Ashutosh Lipton, Mark Chmielewski, Jean |
author_sort | Agrahari, Ashutosh |
collection | PubMed |
description | Peptide-based helical barrels are a noteworthy building block for hierarchical assembly, with a hydrophobic cavity that can serve as a host for cargo. In this study, disulfide-stapled helical barrels were synthesized containing ligands for metal ions on the hydrophilic face of each amphiphilic peptide helix. The major product of the disulfide-stapling reaction was found to be composed of five amphiphilic peptides, thereby going from a 16-amino-acid peptide to a stapled 80-residue protein in one step. The structure of this pentamer, 5HB1, was optimized in silico, indicating a significant hydrophobic cavity of ~6 Å within a helical barrel. Metal-ion-promoted assembly of the helical barrel building blocks generated higher order assemblies with a three-dimensional (3D) matrix morphology. The matrix was decorated with hydrophobic dyes and His-tagged proteins both before and after assembly, taking advantage of the hydrophobic pocket within the helical barrels and coordination sites within the metal ion-peptide framework. As such, this peptide-based biomaterial has potential for a number of biotechnology applications, including supplying small molecule and protein growth factors during cell and tissue growth within the matrix. |
format | Online Article Text |
id | pubmed-10574645 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105746452023-10-14 Metal-Promoted Higher-Order Assembly of Disulfide-Stapled Helical Barrels Agrahari, Ashutosh Lipton, Mark Chmielewski, Jean Nanomaterials (Basel) Article Peptide-based helical barrels are a noteworthy building block for hierarchical assembly, with a hydrophobic cavity that can serve as a host for cargo. In this study, disulfide-stapled helical barrels were synthesized containing ligands for metal ions on the hydrophilic face of each amphiphilic peptide helix. The major product of the disulfide-stapling reaction was found to be composed of five amphiphilic peptides, thereby going from a 16-amino-acid peptide to a stapled 80-residue protein in one step. The structure of this pentamer, 5HB1, was optimized in silico, indicating a significant hydrophobic cavity of ~6 Å within a helical barrel. Metal-ion-promoted assembly of the helical barrel building blocks generated higher order assemblies with a three-dimensional (3D) matrix morphology. The matrix was decorated with hydrophobic dyes and His-tagged proteins both before and after assembly, taking advantage of the hydrophobic pocket within the helical barrels and coordination sites within the metal ion-peptide framework. As such, this peptide-based biomaterial has potential for a number of biotechnology applications, including supplying small molecule and protein growth factors during cell and tissue growth within the matrix. MDPI 2023-09-26 /pmc/articles/PMC10574645/ /pubmed/37836285 http://dx.doi.org/10.3390/nano13192645 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Agrahari, Ashutosh Lipton, Mark Chmielewski, Jean Metal-Promoted Higher-Order Assembly of Disulfide-Stapled Helical Barrels |
title | Metal-Promoted Higher-Order Assembly of Disulfide-Stapled Helical Barrels |
title_full | Metal-Promoted Higher-Order Assembly of Disulfide-Stapled Helical Barrels |
title_fullStr | Metal-Promoted Higher-Order Assembly of Disulfide-Stapled Helical Barrels |
title_full_unstemmed | Metal-Promoted Higher-Order Assembly of Disulfide-Stapled Helical Barrels |
title_short | Metal-Promoted Higher-Order Assembly of Disulfide-Stapled Helical Barrels |
title_sort | metal-promoted higher-order assembly of disulfide-stapled helical barrels |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574645/ https://www.ncbi.nlm.nih.gov/pubmed/37836285 http://dx.doi.org/10.3390/nano13192645 |
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