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Dynamic Surface Properties of α-Lactalbumin Fibril Dispersions
The dynamic surface properties of aqueous dispersions of α-lactalbumin (ALA) amyloid fibrils differ noticeably from the properties of the fibril dispersions of other globular proteins. As a result, the protocol of the application of ALA fibrils to form stable foams and emulsions has to be deviate fr...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574873/ https://www.ncbi.nlm.nih.gov/pubmed/37836019 http://dx.doi.org/10.3390/polym15193970 |
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author | Noskov, Boris Loglio, Giuseppe Miller, Reinhard Milyaeva, Olga Panaeva, Maria Bykov, Alexey |
author_facet | Noskov, Boris Loglio, Giuseppe Miller, Reinhard Milyaeva, Olga Panaeva, Maria Bykov, Alexey |
author_sort | Noskov, Boris |
collection | PubMed |
description | The dynamic surface properties of aqueous dispersions of α-lactalbumin (ALA) amyloid fibrils differ noticeably from the properties of the fibril dispersions of other globular proteins. As a result, the protocol of the application of ALA fibrils to form stable foams and emulsions has to be deviate from that of other protein fibrils. Unlike the fibrils of β-lactoglobulin and lysozyme, ALA fibrils can be easily purified from hydrolyzed peptides and native protein molecules. The application of the oscillating barrier method shows that the dynamic surface elasticity of ALA fibril dispersions exceeds the surface elasticity of native protein solutions at pH 2. ALA fibrils proved to be stable at this pH, but the stability breaks at higher pH levels when the fibrils start to release small peptides of high surface activity. As a result, the dynamic surface properties of ALA coincide with those of native protein solutions. The ionic strength strongly influences the adsorption kinetics of both fibril dispersions and native protein solutions but have almost no impact on the structure of the adsorption layers. |
format | Online Article Text |
id | pubmed-10574873 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-105748732023-10-14 Dynamic Surface Properties of α-Lactalbumin Fibril Dispersions Noskov, Boris Loglio, Giuseppe Miller, Reinhard Milyaeva, Olga Panaeva, Maria Bykov, Alexey Polymers (Basel) Article The dynamic surface properties of aqueous dispersions of α-lactalbumin (ALA) amyloid fibrils differ noticeably from the properties of the fibril dispersions of other globular proteins. As a result, the protocol of the application of ALA fibrils to form stable foams and emulsions has to be deviate from that of other protein fibrils. Unlike the fibrils of β-lactoglobulin and lysozyme, ALA fibrils can be easily purified from hydrolyzed peptides and native protein molecules. The application of the oscillating barrier method shows that the dynamic surface elasticity of ALA fibril dispersions exceeds the surface elasticity of native protein solutions at pH 2. ALA fibrils proved to be stable at this pH, but the stability breaks at higher pH levels when the fibrils start to release small peptides of high surface activity. As a result, the dynamic surface properties of ALA coincide with those of native protein solutions. The ionic strength strongly influences the adsorption kinetics of both fibril dispersions and native protein solutions but have almost no impact on the structure of the adsorption layers. MDPI 2023-10-02 /pmc/articles/PMC10574873/ /pubmed/37836019 http://dx.doi.org/10.3390/polym15193970 Text en © 2023 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Noskov, Boris Loglio, Giuseppe Miller, Reinhard Milyaeva, Olga Panaeva, Maria Bykov, Alexey Dynamic Surface Properties of α-Lactalbumin Fibril Dispersions |
title | Dynamic Surface Properties of α-Lactalbumin Fibril Dispersions |
title_full | Dynamic Surface Properties of α-Lactalbumin Fibril Dispersions |
title_fullStr | Dynamic Surface Properties of α-Lactalbumin Fibril Dispersions |
title_full_unstemmed | Dynamic Surface Properties of α-Lactalbumin Fibril Dispersions |
title_short | Dynamic Surface Properties of α-Lactalbumin Fibril Dispersions |
title_sort | dynamic surface properties of α-lactalbumin fibril dispersions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10574873/ https://www.ncbi.nlm.nih.gov/pubmed/37836019 http://dx.doi.org/10.3390/polym15193970 |
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