Gold Nanoparticle-Based Colorimetric Sensors: Properties and Application in Detection of Heavy Metals and Biological Molecules

During the last decade, advances have been made in nanotechnology using nanomaterials, leading to improvements in their performance. Gold nanoparticles (AuNPs) have been widely used in the field of sensor analysis and are also combined with certain materials to obtain the desired characteristics. AuNPs are commonly used as colorimetric sensors in detection methods. In developing an ideal sensor, there are certain characteristics that must be met such as selectivity, sensitivity, accuracy, precision, and linearity, among others. Various methods for the synthesis of AuNPs and conjugation with other components have been carried out in order to obtain good characteristics for their application. AuNPs can be applied in the detection of both heavy metals and biological molecules. This review aimed at observing the role of AuNPs in its application. The synthesis of AuNPs for sensors will also be revealed, along with their characteristics suitable for this role. In the application method, the size and shape of the particles must be considered. AuNPs used in heavy metal detection have a particle size of around 15–50 nm; in the detection of biological molecules, the particle size of AuNPs used is 6–35 nm whereas in pharmaceutical compounds for cancer treatment and the detection of other drugs, the particle size used is 12–30 nm. The particle sizes did not correlate with the type of molecules regardless of whether it was a heavy metal, biological molecule, or pharmaceutical compound but depended on the properties of the molecule itself. In general, the best morphology for application in the detection process is a spherical shape to obtain good sensitivity and selectivity based on previous studies. Functionalization of AuNPs with conjugates/receptors can be carried out to increase the stability, sensitivity, selectivity, solubility, and plays a role in detecting biological compounds through conjugating AuNPs with biological molecules.