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Spatiotemporal single-cell tracking analysis in 3D tissues to reveal heterogeneous cellular response to mechanical stimuli

Mechanical stimuli have been recognized as important for tissue maturation, homeostasis and constructing engineered three-dimensional (3D) tissues. However, we know little about the cellular mechanical response in tissues that could be considerably heterogeneous and spatiotemporally dynamic due to t...

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Autores principales: Kasahara, Keitaro, Muramatsu, Jumpei, Kurashina, Yuta, Miura, Shigenori, Miyata, Shogo, Onoe, Hiroaki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10575577/
https://www.ncbi.nlm.nih.gov/pubmed/37831766
http://dx.doi.org/10.1126/sciadv.adf9917
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author Kasahara, Keitaro
Muramatsu, Jumpei
Kurashina, Yuta
Miura, Shigenori
Miyata, Shogo
Onoe, Hiroaki
author_facet Kasahara, Keitaro
Muramatsu, Jumpei
Kurashina, Yuta
Miura, Shigenori
Miyata, Shogo
Onoe, Hiroaki
author_sort Kasahara, Keitaro
collection PubMed
description Mechanical stimuli have been recognized as important for tissue maturation, homeostasis and constructing engineered three-dimensional (3D) tissues. However, we know little about the cellular mechanical response in tissues that could be considerably heterogeneous and spatiotemporally dynamic due to the complex structure of tissues. Here, we report a spatiotemporal single-cell tracking analysis of in vitro 3D tissues under mechanical stretch, to reveal the heterogeneous cellular behavior by using a developed stretch and optical live imaging system. The system could affect the cellular orientation and directly measure the distance of cells in in vitro 3D myoblast tissues (3DMTs) at the single-cell level. Moreover, we observed the spatiotemporal heterogeneous cellular locomotion and shape changes under mechanical stretch in 3DMTs. This single-cell tracking analysis can become a principal method to investigate the heterogeneous cellular response in tissues and provide insights that conventional analyses have not yet offered.
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spelling pubmed-105755772023-10-14 Spatiotemporal single-cell tracking analysis in 3D tissues to reveal heterogeneous cellular response to mechanical stimuli Kasahara, Keitaro Muramatsu, Jumpei Kurashina, Yuta Miura, Shigenori Miyata, Shogo Onoe, Hiroaki Sci Adv Biomedicine and Life Sciences Mechanical stimuli have been recognized as important for tissue maturation, homeostasis and constructing engineered three-dimensional (3D) tissues. However, we know little about the cellular mechanical response in tissues that could be considerably heterogeneous and spatiotemporally dynamic due to the complex structure of tissues. Here, we report a spatiotemporal single-cell tracking analysis of in vitro 3D tissues under mechanical stretch, to reveal the heterogeneous cellular behavior by using a developed stretch and optical live imaging system. The system could affect the cellular orientation and directly measure the distance of cells in in vitro 3D myoblast tissues (3DMTs) at the single-cell level. Moreover, we observed the spatiotemporal heterogeneous cellular locomotion and shape changes under mechanical stretch in 3DMTs. This single-cell tracking analysis can become a principal method to investigate the heterogeneous cellular response in tissues and provide insights that conventional analyses have not yet offered. American Association for the Advancement of Science 2023-10-13 /pmc/articles/PMC10575577/ /pubmed/37831766 http://dx.doi.org/10.1126/sciadv.adf9917 Text en Copyright © 2023 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Biomedicine and Life Sciences
Kasahara, Keitaro
Muramatsu, Jumpei
Kurashina, Yuta
Miura, Shigenori
Miyata, Shogo
Onoe, Hiroaki
Spatiotemporal single-cell tracking analysis in 3D tissues to reveal heterogeneous cellular response to mechanical stimuli
title Spatiotemporal single-cell tracking analysis in 3D tissues to reveal heterogeneous cellular response to mechanical stimuli
title_full Spatiotemporal single-cell tracking analysis in 3D tissues to reveal heterogeneous cellular response to mechanical stimuli
title_fullStr Spatiotemporal single-cell tracking analysis in 3D tissues to reveal heterogeneous cellular response to mechanical stimuli
title_full_unstemmed Spatiotemporal single-cell tracking analysis in 3D tissues to reveal heterogeneous cellular response to mechanical stimuli
title_short Spatiotemporal single-cell tracking analysis in 3D tissues to reveal heterogeneous cellular response to mechanical stimuli
title_sort spatiotemporal single-cell tracking analysis in 3d tissues to reveal heterogeneous cellular response to mechanical stimuli
topic Biomedicine and Life Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10575577/
https://www.ncbi.nlm.nih.gov/pubmed/37831766
http://dx.doi.org/10.1126/sciadv.adf9917
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