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High-resolution analysis of bound Ca(2+) in neurons and synapses
Calcium (Ca(2+)) is a well-known second messenger in all cells, and is especially relevant for neuronal activity. Neuronal Ca(2+) is found in different forms, with a minority being freely soluble in the cell and more than 99% being bound to proteins. Free Ca(2+) has received much attention over the...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Life Science Alliance LLC
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10575792/ https://www.ncbi.nlm.nih.gov/pubmed/37833073 http://dx.doi.org/10.26508/lsa.202302030 |
Sumario: | Calcium (Ca(2+)) is a well-known second messenger in all cells, and is especially relevant for neuronal activity. Neuronal Ca(2+) is found in different forms, with a minority being freely soluble in the cell and more than 99% being bound to proteins. Free Ca(2+) has received much attention over the last few decades, but protein-bound Ca(2+) has been difficult to analyze. Here, we introduce correlative fluorescence and nanoscale secondary ion mass spectrometry imaging as a tool to describe bound Ca(2+). As expected, bound Ca(2+) is ubiquitous. It does not correlate to free Ca(2+) dynamics at the whole-neuron level, but does correlate significantly to the intensity of markers for GABAergic pre-synapse and glutamatergic post-synapses. In contrast, a negative correlation to pre-synaptic activity was observed, with lower levels of bound Ca(2+) observed in the more active synapses. We conclude that bound Ca(2+) may regulate neuronal activity and should receive more attention in the future. |
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