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Extracellular vesicles could be a putative posttranscriptional regulatory mechanism that shapes intracellular RNA levels in Plasmodium falciparum

Plasmodium falciparum secretes extracellular vesicles (PfEVs) that contain parasite-derived RNA. However, the significance of the secreted RNA remains unexplored. Here, we compare secreted and intracellular RNA from asexual cultures of six P. falciparum lines. We find that secretion of RNA via extra...

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Autores principales: Kioko, Mwikali, Pance, Alena, Mwangi, Shaban, Goulding, David, Kemp, Alison, Rono, Martin, Ochola-Oyier, Lynette Isabella, Bull, Pete C., Bejon, Philip, Rayner, Julian C., Abdi, Abdirahman I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10575976/
https://www.ncbi.nlm.nih.gov/pubmed/37833314
http://dx.doi.org/10.1038/s41467-023-42103-x
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author Kioko, Mwikali
Pance, Alena
Mwangi, Shaban
Goulding, David
Kemp, Alison
Rono, Martin
Ochola-Oyier, Lynette Isabella
Bull, Pete C.
Bejon, Philip
Rayner, Julian C.
Abdi, Abdirahman I.
author_facet Kioko, Mwikali
Pance, Alena
Mwangi, Shaban
Goulding, David
Kemp, Alison
Rono, Martin
Ochola-Oyier, Lynette Isabella
Bull, Pete C.
Bejon, Philip
Rayner, Julian C.
Abdi, Abdirahman I.
author_sort Kioko, Mwikali
collection PubMed
description Plasmodium falciparum secretes extracellular vesicles (PfEVs) that contain parasite-derived RNA. However, the significance of the secreted RNA remains unexplored. Here, we compare secreted and intracellular RNA from asexual cultures of six P. falciparum lines. We find that secretion of RNA via extracellular vesicles is not only periodic throughout the asexual intraerythrocytic developmental cycle but is also highly conserved across P. falciparum isolates. We further demonstrate that the phases of RNA secreted via extracellular vesicles are discernibly shifted compared to those of the intracellular RNA within the secreting whole parasite. Finally, transcripts of genes with no known function during the asexual intraerythrocytic developmental cycle are enriched in PfEVs compared to the whole parasite. We conclude that the secretion of extracellular vesicles could be a putative posttranscriptional RNA regulation mechanism that is part of or synergise the classic RNA decay processes to maintain intracellular RNA levels in P. falciparum.
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spelling pubmed-105759762023-10-15 Extracellular vesicles could be a putative posttranscriptional regulatory mechanism that shapes intracellular RNA levels in Plasmodium falciparum Kioko, Mwikali Pance, Alena Mwangi, Shaban Goulding, David Kemp, Alison Rono, Martin Ochola-Oyier, Lynette Isabella Bull, Pete C. Bejon, Philip Rayner, Julian C. Abdi, Abdirahman I. Nat Commun Article Plasmodium falciparum secretes extracellular vesicles (PfEVs) that contain parasite-derived RNA. However, the significance of the secreted RNA remains unexplored. Here, we compare secreted and intracellular RNA from asexual cultures of six P. falciparum lines. We find that secretion of RNA via extracellular vesicles is not only periodic throughout the asexual intraerythrocytic developmental cycle but is also highly conserved across P. falciparum isolates. We further demonstrate that the phases of RNA secreted via extracellular vesicles are discernibly shifted compared to those of the intracellular RNA within the secreting whole parasite. Finally, transcripts of genes with no known function during the asexual intraerythrocytic developmental cycle are enriched in PfEVs compared to the whole parasite. We conclude that the secretion of extracellular vesicles could be a putative posttranscriptional RNA regulation mechanism that is part of or synergise the classic RNA decay processes to maintain intracellular RNA levels in P. falciparum. Nature Publishing Group UK 2023-10-13 /pmc/articles/PMC10575976/ /pubmed/37833314 http://dx.doi.org/10.1038/s41467-023-42103-x Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Kioko, Mwikali
Pance, Alena
Mwangi, Shaban
Goulding, David
Kemp, Alison
Rono, Martin
Ochola-Oyier, Lynette Isabella
Bull, Pete C.
Bejon, Philip
Rayner, Julian C.
Abdi, Abdirahman I.
Extracellular vesicles could be a putative posttranscriptional regulatory mechanism that shapes intracellular RNA levels in Plasmodium falciparum
title Extracellular vesicles could be a putative posttranscriptional regulatory mechanism that shapes intracellular RNA levels in Plasmodium falciparum
title_full Extracellular vesicles could be a putative posttranscriptional regulatory mechanism that shapes intracellular RNA levels in Plasmodium falciparum
title_fullStr Extracellular vesicles could be a putative posttranscriptional regulatory mechanism that shapes intracellular RNA levels in Plasmodium falciparum
title_full_unstemmed Extracellular vesicles could be a putative posttranscriptional regulatory mechanism that shapes intracellular RNA levels in Plasmodium falciparum
title_short Extracellular vesicles could be a putative posttranscriptional regulatory mechanism that shapes intracellular RNA levels in Plasmodium falciparum
title_sort extracellular vesicles could be a putative posttranscriptional regulatory mechanism that shapes intracellular rna levels in plasmodium falciparum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10575976/
https://www.ncbi.nlm.nih.gov/pubmed/37833314
http://dx.doi.org/10.1038/s41467-023-42103-x
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