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Transcriptomic analysis of the antimicrobial activity of prodigiosin against Cutibacterium acnes

Prodigiosin, a red pigment produced by Hahella chejuensis, a marine-derived microorganism, has several biological functions, including antimicrobial activity and inflammatory relief. In this study, the antibacterial activity of prodigiosin against skin microorganisms was explored. Paper disc assay o...

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Autores principales: Kim, Hyun Ju, Lee, Moo-Seung, Jeong, Se Kyoo, Lee, Sang Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10576067/
https://www.ncbi.nlm.nih.gov/pubmed/37833344
http://dx.doi.org/10.1038/s41598-023-44612-7
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author Kim, Hyun Ju
Lee, Moo-Seung
Jeong, Se Kyoo
Lee, Sang Jun
author_facet Kim, Hyun Ju
Lee, Moo-Seung
Jeong, Se Kyoo
Lee, Sang Jun
author_sort Kim, Hyun Ju
collection PubMed
description Prodigiosin, a red pigment produced by Hahella chejuensis, a marine-derived microorganism, has several biological functions, including antimicrobial activity and inflammatory relief. In this study, the antibacterial activity of prodigiosin against skin microorganisms was explored. Paper disc assay on skin bacterial cells revealed that Cutibacterium acnes related to acne vulgaris highly susceptible to prodigiosin. MIC (Minimal Inhibitory Concentration) and MBC (Minimal Bactericidal Concentration) were determined on Cutibacterium species. The RNA-seq analysis of prodigiosin-treated C. acnes cells was performed to understand the antibacterial mechanism of prodigiosin. Among changes in the expression of hundreds of genes, the expression of a stress-responsive sigma factor encoded by sigB increased. Conversely, the gene expression of cell wall biosynthesis and energy metabolism was inhibited by prodigiosin. Specifically, the expression of genes related to the metabolism of porphyrin, a pro-inflammatory metabolite, was significantly reduced. Therefore, prodigiosin could be used to control C. acnes. Our study provided new insights into the antimicrobial mechanism of prodigiosin against C. acnes strains.
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spelling pubmed-105760672023-10-15 Transcriptomic analysis of the antimicrobial activity of prodigiosin against Cutibacterium acnes Kim, Hyun Ju Lee, Moo-Seung Jeong, Se Kyoo Lee, Sang Jun Sci Rep Article Prodigiosin, a red pigment produced by Hahella chejuensis, a marine-derived microorganism, has several biological functions, including antimicrobial activity and inflammatory relief. In this study, the antibacterial activity of prodigiosin against skin microorganisms was explored. Paper disc assay on skin bacterial cells revealed that Cutibacterium acnes related to acne vulgaris highly susceptible to prodigiosin. MIC (Minimal Inhibitory Concentration) and MBC (Minimal Bactericidal Concentration) were determined on Cutibacterium species. The RNA-seq analysis of prodigiosin-treated C. acnes cells was performed to understand the antibacterial mechanism of prodigiosin. Among changes in the expression of hundreds of genes, the expression of a stress-responsive sigma factor encoded by sigB increased. Conversely, the gene expression of cell wall biosynthesis and energy metabolism was inhibited by prodigiosin. Specifically, the expression of genes related to the metabolism of porphyrin, a pro-inflammatory metabolite, was significantly reduced. Therefore, prodigiosin could be used to control C. acnes. Our study provided new insights into the antimicrobial mechanism of prodigiosin against C. acnes strains. Nature Publishing Group UK 2023-10-13 /pmc/articles/PMC10576067/ /pubmed/37833344 http://dx.doi.org/10.1038/s41598-023-44612-7 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Kim, Hyun Ju
Lee, Moo-Seung
Jeong, Se Kyoo
Lee, Sang Jun
Transcriptomic analysis of the antimicrobial activity of prodigiosin against Cutibacterium acnes
title Transcriptomic analysis of the antimicrobial activity of prodigiosin against Cutibacterium acnes
title_full Transcriptomic analysis of the antimicrobial activity of prodigiosin against Cutibacterium acnes
title_fullStr Transcriptomic analysis of the antimicrobial activity of prodigiosin against Cutibacterium acnes
title_full_unstemmed Transcriptomic analysis of the antimicrobial activity of prodigiosin against Cutibacterium acnes
title_short Transcriptomic analysis of the antimicrobial activity of prodigiosin against Cutibacterium acnes
title_sort transcriptomic analysis of the antimicrobial activity of prodigiosin against cutibacterium acnes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10576067/
https://www.ncbi.nlm.nih.gov/pubmed/37833344
http://dx.doi.org/10.1038/s41598-023-44612-7
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