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Efficient multiple gene knockout in Colletotrichum higginsianum via CRISPR/Cas9 ribonucleoprotein and URA3‐based marker recycling

Colletotrichum higginsianum is a hemibiotrophic pathogen that causes anthracnose disease on crucifer hosts, including Arabidopsis thaliana. Despite the availability of genomic and transcriptomic information and the ability to transform both organisms, identifying C. higginsianum genes involved in vi...

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Detalles Bibliográficos
Autores principales:	Yonehara, Katsuma, Kumakura, Naoyoshi, Motoyama, Takayuki, Ishihama, Nobuaki, Dallery, Jean‐Félix, O'Connell, Richard, Shirasu, Ken
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2023
Materias:	Technical Advance
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10576178/ https://www.ncbi.nlm.nih.gov/pubmed/37522511 http://dx.doi.org/10.1111/mpp.13378

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