Cargando…

Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts

BACKGROUND: Individual host susceptibility is believed to be a risk factor in the interaction between the host and the parasite. Since studying time series in humans is limited, animal models are replaced. AIM: This study aims to explore and compare the pattern of inflammatory cell types along the b...

Descripción completa

Detalles Bibliográficos
Autores principales: Thongrin, Theerayut, Suyapoh, Watcharapol, Wendo, WoroDanur, Tangkawattana, Prasarn, Sukon, Peerapol, Salao, Kanin, Suttiprapa, Sutas, Saichua, Prasert, Tangkawatana, Sirikachorn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Faculty of Veterinary Medicine 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10576576/
https://www.ncbi.nlm.nih.gov/pubmed/37842106
http://dx.doi.org/10.5455/OVJ.2023.v13.i9.11
_version_ 1785121145582780416
author Thongrin, Theerayut
Suyapoh, Watcharapol
Wendo, WoroDanur
Tangkawattana, Prasarn
Sukon, Peerapol
Salao, Kanin
Suttiprapa, Sutas
Saichua, Prasert
Tangkawatana, Sirikachorn
author_facet Thongrin, Theerayut
Suyapoh, Watcharapol
Wendo, WoroDanur
Tangkawattana, Prasarn
Sukon, Peerapol
Salao, Kanin
Suttiprapa, Sutas
Saichua, Prasert
Tangkawatana, Sirikachorn
author_sort Thongrin, Theerayut
collection PubMed
description BACKGROUND: Individual host susceptibility is believed to be a risk factor in the interaction between the host and the parasite. Since studying time series in humans is limited, animal models are replaced. AIM: This study aims to explore and compare the pattern of inflammatory cell types along the biliary tract and their association with proliferative lesions in the early development of cholangiocarcinoma from susceptible and nonsusceptible animal models. METHODS: Thirty male Syrian golden hamsters and 30 BALB/c mice, serving as the susceptible and nonsusceptible animal models, were used in this comparative study. The animals were infected with 50 Opisthorchis viverrini metacercariae via gastric intubation. At days 1, 2, 7, 14, 28, and 56 postinfection (p.i.), five animals were randomly selected from each group and humanely sacrificed. The hepatobiliary tissues were collected and processed for histopathological study. Histochemical and immunohistochemical staining were applied to differentiate the inflammatory cell types. Kruskal–Wallis and Mann–Whitney tests were applied to assess all semi-quantitative and quantitative variables. The correlation between each variable was also analyzed using Spearman rank at a p-value < 0.05. RESULTS: The results demonstrated that mice had different patterns of infiltrating cell types when compared to hamsters. This suggested that the cellular response to the infection in mice occurred earlier than that in hamsters. The response in mice reached its peak at D7 to D14 and then rapidly declined at D28. In contrast, although the inflammatory response in hamsters started slowly, the response reached the peak at D28 and maintained a high level until D56. Significant differences in the number of inflammatory cells between mice and hamsters were seen at D1 (p = 0.047), D7 (p = 0.049), D28 (p = 0.040), and D56 (p < 0.040). CONCLUSION: The inflammatory responses to O. viverrini infection in the nonsusceptible animal model occurred and declined earlier while the response in the susceptible animal model occurred later in a gradual manner. Both rodents are suitable animal models for the studies of opisthorchiasis susceptibility.
format Online
Article
Text
id pubmed-10576576
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Faculty of Veterinary Medicine
record_format MEDLINE/PubMed
spelling pubmed-105765762023-10-15 Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts Thongrin, Theerayut Suyapoh, Watcharapol Wendo, WoroDanur Tangkawattana, Prasarn Sukon, Peerapol Salao, Kanin Suttiprapa, Sutas Saichua, Prasert Tangkawatana, Sirikachorn Open Vet J Original Research BACKGROUND: Individual host susceptibility is believed to be a risk factor in the interaction between the host and the parasite. Since studying time series in humans is limited, animal models are replaced. AIM: This study aims to explore and compare the pattern of inflammatory cell types along the biliary tract and their association with proliferative lesions in the early development of cholangiocarcinoma from susceptible and nonsusceptible animal models. METHODS: Thirty male Syrian golden hamsters and 30 BALB/c mice, serving as the susceptible and nonsusceptible animal models, were used in this comparative study. The animals were infected with 50 Opisthorchis viverrini metacercariae via gastric intubation. At days 1, 2, 7, 14, 28, and 56 postinfection (p.i.), five animals were randomly selected from each group and humanely sacrificed. The hepatobiliary tissues were collected and processed for histopathological study. Histochemical and immunohistochemical staining were applied to differentiate the inflammatory cell types. Kruskal–Wallis and Mann–Whitney tests were applied to assess all semi-quantitative and quantitative variables. The correlation between each variable was also analyzed using Spearman rank at a p-value < 0.05. RESULTS: The results demonstrated that mice had different patterns of infiltrating cell types when compared to hamsters. This suggested that the cellular response to the infection in mice occurred earlier than that in hamsters. The response in mice reached its peak at D7 to D14 and then rapidly declined at D28. In contrast, although the inflammatory response in hamsters started slowly, the response reached the peak at D28 and maintained a high level until D56. Significant differences in the number of inflammatory cells between mice and hamsters were seen at D1 (p = 0.047), D7 (p = 0.049), D28 (p = 0.040), and D56 (p < 0.040). CONCLUSION: The inflammatory responses to O. viverrini infection in the nonsusceptible animal model occurred and declined earlier while the response in the susceptible animal model occurred later in a gradual manner. Both rodents are suitable animal models for the studies of opisthorchiasis susceptibility. Faculty of Veterinary Medicine 2023-09 2023-09-30 /pmc/articles/PMC10576576/ /pubmed/37842106 http://dx.doi.org/10.5455/OVJ.2023.v13.i9.11 Text en https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Research
Thongrin, Theerayut
Suyapoh, Watcharapol
Wendo, WoroDanur
Tangkawattana, Prasarn
Sukon, Peerapol
Salao, Kanin
Suttiprapa, Sutas
Saichua, Prasert
Tangkawatana, Sirikachorn
Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts
title Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts
title_full Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts
title_fullStr Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts
title_full_unstemmed Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts
title_short Inflammatory cell responses in biliary mucosa during Opisthorchis viverrini infection: Insights into susceptibility differences among hosts
title_sort inflammatory cell responses in biliary mucosa during opisthorchis viverrini infection: insights into susceptibility differences among hosts
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10576576/
https://www.ncbi.nlm.nih.gov/pubmed/37842106
http://dx.doi.org/10.5455/OVJ.2023.v13.i9.11
work_keys_str_mv AT thongrintheerayut inflammatorycellresponsesinbiliarymucosaduringopisthorchisviverriniinfectioninsightsintosusceptibilitydifferencesamonghosts
AT suyapohwatcharapol inflammatorycellresponsesinbiliarymucosaduringopisthorchisviverriniinfectioninsightsintosusceptibilitydifferencesamonghosts
AT wendoworodanur inflammatorycellresponsesinbiliarymucosaduringopisthorchisviverriniinfectioninsightsintosusceptibilitydifferencesamonghosts
AT tangkawattanaprasarn inflammatorycellresponsesinbiliarymucosaduringopisthorchisviverriniinfectioninsightsintosusceptibilitydifferencesamonghosts
AT sukonpeerapol inflammatorycellresponsesinbiliarymucosaduringopisthorchisviverriniinfectioninsightsintosusceptibilitydifferencesamonghosts
AT salaokanin inflammatorycellresponsesinbiliarymucosaduringopisthorchisviverriniinfectioninsightsintosusceptibilitydifferencesamonghosts
AT suttiprapasutas inflammatorycellresponsesinbiliarymucosaduringopisthorchisviverriniinfectioninsightsintosusceptibilitydifferencesamonghosts
AT saichuaprasert inflammatorycellresponsesinbiliarymucosaduringopisthorchisviverriniinfectioninsightsintosusceptibilitydifferencesamonghosts
AT tangkawatanasirikachorn inflammatorycellresponsesinbiliarymucosaduringopisthorchisviverriniinfectioninsightsintosusceptibilitydifferencesamonghosts