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Stenotrophomonas rhizophila Ep2.2 inhibits growth of Botrytis cinerea through the emission of volatile organic compounds, restricts leaf infection and primes defense genes

The bacterium Stenotrophomonas rhizophila is known to be beneficial for plants and has been frequently isolated from the rhizosphere of crops. In the present work, we isolated from the phyllosphere of an ornamental plant an epiphytic strain of S. rhizophila that we named Ep2.2 and investigated its p...

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Autores principales: Raio, Aida, Brilli, Federico, Neri, Luisa, Baraldi, Rita, Orlando, Francesca, Pugliesi, Claudio, Chen, Xiaoyulong, Baccelli, Ivan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10577304/
https://www.ncbi.nlm.nih.gov/pubmed/37849842
http://dx.doi.org/10.3389/fpls.2023.1235669
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author Raio, Aida
Brilli, Federico
Neri, Luisa
Baraldi, Rita
Orlando, Francesca
Pugliesi, Claudio
Chen, Xiaoyulong
Baccelli, Ivan
author_facet Raio, Aida
Brilli, Federico
Neri, Luisa
Baraldi, Rita
Orlando, Francesca
Pugliesi, Claudio
Chen, Xiaoyulong
Baccelli, Ivan
author_sort Raio, Aida
collection PubMed
description The bacterium Stenotrophomonas rhizophila is known to be beneficial for plants and has been frequently isolated from the rhizosphere of crops. In the present work, we isolated from the phyllosphere of an ornamental plant an epiphytic strain of S. rhizophila that we named Ep2.2 and investigated its possible application in crop protection. Compared to S. maltophilia LMG 958, a well-known plant beneficial species which behaves as opportunistic human pathogen, S. rhizophila Ep2.2 showed distinctive features, such as different motility, a generally reduced capacity to use carbon sources, a greater sensitivity to fusidic acid and potassium tellurite, and the inability to grow at the human body temperature. S. rhizophila Ep2.2 was able to inhibit in vitro growth of the plant pathogenic fungi Alternaria alternata and Botrytis cinerea through the emission of volatile compounds. Simultaneous PTR-MS and GC-MS analyses revealed the emission, by S. rhizophila Ep2.2, of volatile organic compounds (VOCs) with well-documented antifungal activity, such as furans, sulphur-containing compounds and terpenes. When sprayed on tomato leaves and plants, S. rhizophila Ep2.2 was able to restrict B. cinerea infection and to prime the expression of Pti5, GluA and PR1 plant defense genes.
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spelling pubmed-105773042023-10-17 Stenotrophomonas rhizophila Ep2.2 inhibits growth of Botrytis cinerea through the emission of volatile organic compounds, restricts leaf infection and primes defense genes Raio, Aida Brilli, Federico Neri, Luisa Baraldi, Rita Orlando, Francesca Pugliesi, Claudio Chen, Xiaoyulong Baccelli, Ivan Front Plant Sci Plant Science The bacterium Stenotrophomonas rhizophila is known to be beneficial for plants and has been frequently isolated from the rhizosphere of crops. In the present work, we isolated from the phyllosphere of an ornamental plant an epiphytic strain of S. rhizophila that we named Ep2.2 and investigated its possible application in crop protection. Compared to S. maltophilia LMG 958, a well-known plant beneficial species which behaves as opportunistic human pathogen, S. rhizophila Ep2.2 showed distinctive features, such as different motility, a generally reduced capacity to use carbon sources, a greater sensitivity to fusidic acid and potassium tellurite, and the inability to grow at the human body temperature. S. rhizophila Ep2.2 was able to inhibit in vitro growth of the plant pathogenic fungi Alternaria alternata and Botrytis cinerea through the emission of volatile compounds. Simultaneous PTR-MS and GC-MS analyses revealed the emission, by S. rhizophila Ep2.2, of volatile organic compounds (VOCs) with well-documented antifungal activity, such as furans, sulphur-containing compounds and terpenes. When sprayed on tomato leaves and plants, S. rhizophila Ep2.2 was able to restrict B. cinerea infection and to prime the expression of Pti5, GluA and PR1 plant defense genes. Frontiers Media S.A. 2023-10-02 /pmc/articles/PMC10577304/ /pubmed/37849842 http://dx.doi.org/10.3389/fpls.2023.1235669 Text en Copyright © 2023 Raio, Brilli, Neri, Baraldi, Orlando, Pugliesi, Chen and Baccelli https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Raio, Aida
Brilli, Federico
Neri, Luisa
Baraldi, Rita
Orlando, Francesca
Pugliesi, Claudio
Chen, Xiaoyulong
Baccelli, Ivan
Stenotrophomonas rhizophila Ep2.2 inhibits growth of Botrytis cinerea through the emission of volatile organic compounds, restricts leaf infection and primes defense genes
title Stenotrophomonas rhizophila Ep2.2 inhibits growth of Botrytis cinerea through the emission of volatile organic compounds, restricts leaf infection and primes defense genes
title_full Stenotrophomonas rhizophila Ep2.2 inhibits growth of Botrytis cinerea through the emission of volatile organic compounds, restricts leaf infection and primes defense genes
title_fullStr Stenotrophomonas rhizophila Ep2.2 inhibits growth of Botrytis cinerea through the emission of volatile organic compounds, restricts leaf infection and primes defense genes
title_full_unstemmed Stenotrophomonas rhizophila Ep2.2 inhibits growth of Botrytis cinerea through the emission of volatile organic compounds, restricts leaf infection and primes defense genes
title_short Stenotrophomonas rhizophila Ep2.2 inhibits growth of Botrytis cinerea through the emission of volatile organic compounds, restricts leaf infection and primes defense genes
title_sort stenotrophomonas rhizophila ep2.2 inhibits growth of botrytis cinerea through the emission of volatile organic compounds, restricts leaf infection and primes defense genes
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10577304/
https://www.ncbi.nlm.nih.gov/pubmed/37849842
http://dx.doi.org/10.3389/fpls.2023.1235669
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