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CH02 peptide promotes ex vivo expansion of umbilical cord blood-derived CD34 (+) hematopoietic stem/progenitor cells : CH02 peptide promotes CD34 (+) UCB-HSPC ex vivo expansion

Umbilical cord blood (UCB) is an advantageous source for hematopoietic stem/progenitor cell (HSPC) transplantation, yet the current strategies for large-scale and cost-effective UCB-HSPC preparation are still unavailable. To overcome these obstacles, we systematically evaluate the feasibility of our...

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Detalles Bibliográficos
Autores principales: Yang, Yiqi, Zhang, Bihui, Xie, Junye, Li, Jingsheng, Liu, Jia, Liu, Rongzhan, Zhang, Linhao, Zhang, Jinting, Su, Zijian, Li, Fu, Zhang, Leisheng, Hong, An, Chen, Xiaojia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10577473/
https://www.ncbi.nlm.nih.gov/pubmed/37381672
http://dx.doi.org/10.3724/abbs.2023047
Descripción
Sumario:Umbilical cord blood (UCB) is an advantageous source for hematopoietic stem/progenitor cell (HSPC) transplantation, yet the current strategies for large-scale and cost-effective UCB-HSPC preparation are still unavailable. To overcome these obstacles, we systematically evaluate the feasibility of our newly identified CH02 peptide for ex vivo expansion of CD34 (+) UCB-HSPCs. We herein report that the CH02 peptide is specifically enriched in HSPC proliferation via activating the FLT3 signaling. Notably, the CH02-based cocktails are adequate for boosting 12-fold ex vivo expansion of UCB-HSPCs. Meanwhile, CH02-preconditioned UCB-HSPCs manifest preferable efficacy upon wound healing in diabetic mice via bidirectional orchestration of proinflammatory and anti-inflammatory factors. Together, our data indicate the advantages of the CH02-based strategy for ex vivo expansion of CD34 (+) UCB-HSPCs, which will provide new strategies for further development of large-scale HSPC preparation for clinical purposes.