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Comparison of Kato–Katz, PCR and coproantigen for the diagnosis of Taenia solium taeniasis
Four methods were compared for the diagnosis of human taeniasis caused by Taenia solium. Fecal samples from persons living in a T. solium endemic region of Madagascar were examined for taeniid eggs by the Kato–Katz method. Subsequently, samples positive (n = 16) and negative (n = 200) for T. solium...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Cambridge University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10577650/ https://www.ncbi.nlm.nih.gov/pubmed/37621007 http://dx.doi.org/10.1017/S0031182023000690 |
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author | Lightowlers, Marshall W. Mananjara, Diana Edithe Andria Rakotoarinoro, Mihajamanana Rakotoarison, Valisoa C. Raliniaina, Modestine Rasamoelina-Andriamanivo, Harentsoaniaina Gauci, Charles G. Jabbar, Abdul Mwape, Kabemba E. Donadeu, Meritxell Ramiandrasoa, Noromanana Sylvia Nely, Jose Alphonse |
author_facet | Lightowlers, Marshall W. Mananjara, Diana Edithe Andria Rakotoarinoro, Mihajamanana Rakotoarison, Valisoa C. Raliniaina, Modestine Rasamoelina-Andriamanivo, Harentsoaniaina Gauci, Charles G. Jabbar, Abdul Mwape, Kabemba E. Donadeu, Meritxell Ramiandrasoa, Noromanana Sylvia Nely, Jose Alphonse |
author_sort | Lightowlers, Marshall W. |
collection | PubMed |
description | Four methods were compared for the diagnosis of human taeniasis caused by Taenia solium. Fecal samples from persons living in a T. solium endemic region of Madagascar were examined for taeniid eggs by the Kato–Katz method. Subsequently, samples positive (n = 16) and negative (n = 200) for T. solium eggs were examined by (i) amplification of the fragment of small subunit of the mitochondrial ribosomal RNA (rrnS) gene using conventional polymerase chain reaction (PCR) and (ii) a nested PCR of a fragment of the T. solium Tso31 gene. Additionally, 12 egg-positive and all egg-negative samples were tested for coproantigen detection. A further 9 egg-positive fecal samples were examined using both PCRs. Of the 12 egg-positive samples tested by PCRs and coproantigen methods, 9 (75%) were positive by rrnS PCR, 3 (25%) using Tso31-nested PCR and 9 (75%) by coproantigen testing. None of the 200 egg-negative fecal samples was positive in either rrnS or Tso31-nested PCR. Twenty of the 25 egg-positive samples (80%) were positive in rrnS PCR, and DNA sequencing of PCR amplicons was obtained from 18 samples, all confirmed to be T. solium. Twelve of the 25 egg-positive samples (48%) were positive in the Tso31-nested PCR, all of which were also positive by rrnS PCR. It is suggested that species-specific diagnosis of T. solium taeniasis may be achieved by either coprological examination to detect eggs or coproantigen testing, followed by rrnS PCR and DNA sequencing to confirm the tapeworm species in egg-positive or coproantigen-positive samples. |
format | Online Article Text |
id | pubmed-10577650 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Cambridge University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-105776502023-10-17 Comparison of Kato–Katz, PCR and coproantigen for the diagnosis of Taenia solium taeniasis Lightowlers, Marshall W. Mananjara, Diana Edithe Andria Rakotoarinoro, Mihajamanana Rakotoarison, Valisoa C. Raliniaina, Modestine Rasamoelina-Andriamanivo, Harentsoaniaina Gauci, Charles G. Jabbar, Abdul Mwape, Kabemba E. Donadeu, Meritxell Ramiandrasoa, Noromanana Sylvia Nely, Jose Alphonse Parasitology Research Article Four methods were compared for the diagnosis of human taeniasis caused by Taenia solium. Fecal samples from persons living in a T. solium endemic region of Madagascar were examined for taeniid eggs by the Kato–Katz method. Subsequently, samples positive (n = 16) and negative (n = 200) for T. solium eggs were examined by (i) amplification of the fragment of small subunit of the mitochondrial ribosomal RNA (rrnS) gene using conventional polymerase chain reaction (PCR) and (ii) a nested PCR of a fragment of the T. solium Tso31 gene. Additionally, 12 egg-positive and all egg-negative samples were tested for coproantigen detection. A further 9 egg-positive fecal samples were examined using both PCRs. Of the 12 egg-positive samples tested by PCRs and coproantigen methods, 9 (75%) were positive by rrnS PCR, 3 (25%) using Tso31-nested PCR and 9 (75%) by coproantigen testing. None of the 200 egg-negative fecal samples was positive in either rrnS or Tso31-nested PCR. Twenty of the 25 egg-positive samples (80%) were positive in rrnS PCR, and DNA sequencing of PCR amplicons was obtained from 18 samples, all confirmed to be T. solium. Twelve of the 25 egg-positive samples (48%) were positive in the Tso31-nested PCR, all of which were also positive by rrnS PCR. It is suggested that species-specific diagnosis of T. solium taeniasis may be achieved by either coprological examination to detect eggs or coproantigen testing, followed by rrnS PCR and DNA sequencing to confirm the tapeworm species in egg-positive or coproantigen-positive samples. Cambridge University Press 2023-09 2023-07-31 /pmc/articles/PMC10577650/ /pubmed/37621007 http://dx.doi.org/10.1017/S0031182023000690 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/This is an Open Access article, distributed under the terms of the Creative Commons Attribution licence (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted re-use, distribution and reproduction, provided the original article is properly cited. |
spellingShingle | Research Article Lightowlers, Marshall W. Mananjara, Diana Edithe Andria Rakotoarinoro, Mihajamanana Rakotoarison, Valisoa C. Raliniaina, Modestine Rasamoelina-Andriamanivo, Harentsoaniaina Gauci, Charles G. Jabbar, Abdul Mwape, Kabemba E. Donadeu, Meritxell Ramiandrasoa, Noromanana Sylvia Nely, Jose Alphonse Comparison of Kato–Katz, PCR and coproantigen for the diagnosis of Taenia solium taeniasis |
title | Comparison of Kato–Katz, PCR and coproantigen for the diagnosis of Taenia solium taeniasis |
title_full | Comparison of Kato–Katz, PCR and coproantigen for the diagnosis of Taenia solium taeniasis |
title_fullStr | Comparison of Kato–Katz, PCR and coproantigen for the diagnosis of Taenia solium taeniasis |
title_full_unstemmed | Comparison of Kato–Katz, PCR and coproantigen for the diagnosis of Taenia solium taeniasis |
title_short | Comparison of Kato–Katz, PCR and coproantigen for the diagnosis of Taenia solium taeniasis |
title_sort | comparison of kato–katz, pcr and coproantigen for the diagnosis of taenia solium taeniasis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10577650/ https://www.ncbi.nlm.nih.gov/pubmed/37621007 http://dx.doi.org/10.1017/S0031182023000690 |
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