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Cryo-electron tomography to study viral infection

Developments in cryo-electron microscopy (cryo-EM) have been interwoven with the study of viruses ever since its first applications to biological systems. Following the success of single particle cryo-EM in the last decade, cryo-electron tomography (cryo-ET) is now rapidly maturing as a technology a...

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Detalles Bibliográficos
Autores principales: Graham, Miles, Zhang, Peijun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10578967/
https://www.ncbi.nlm.nih.gov/pubmed/37560901
http://dx.doi.org/10.1042/BST20230103
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author Graham, Miles
Zhang, Peijun
author_facet Graham, Miles
Zhang, Peijun
author_sort Graham, Miles
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description Developments in cryo-electron microscopy (cryo-EM) have been interwoven with the study of viruses ever since its first applications to biological systems. Following the success of single particle cryo-EM in the last decade, cryo-electron tomography (cryo-ET) is now rapidly maturing as a technology and catalysing great advancement in structural virology as its application broadens. In this review, we provide an overview of the use of cryo-ET to study viral infection biology, discussing the key workflows and strategies used in the field. We highlight the vast body of studies performed on purified viruses and virus-like particles (VLPs), as well as discussing how cryo-ET can characterise host–virus interactions and membrane fusion events. We further discuss the importance of in situ cellular imaging in revealing previously unattainable details of infection and highlight the need for validation of high-resolution findings from purified ex situ systems. We give perspectives for future developments to achieve the full potential of cryo-ET to characterise the molecular processes of viral infection.
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spelling pubmed-105789672023-10-16 Cryo-electron tomography to study viral infection Graham, Miles Zhang, Peijun Biochem Soc Trans Review Articles Developments in cryo-electron microscopy (cryo-EM) have been interwoven with the study of viruses ever since its first applications to biological systems. Following the success of single particle cryo-EM in the last decade, cryo-electron tomography (cryo-ET) is now rapidly maturing as a technology and catalysing great advancement in structural virology as its application broadens. In this review, we provide an overview of the use of cryo-ET to study viral infection biology, discussing the key workflows and strategies used in the field. We highlight the vast body of studies performed on purified viruses and virus-like particles (VLPs), as well as discussing how cryo-ET can characterise host–virus interactions and membrane fusion events. We further discuss the importance of in situ cellular imaging in revealing previously unattainable details of infection and highlight the need for validation of high-resolution findings from purified ex situ systems. We give perspectives for future developments to achieve the full potential of cryo-ET to characterise the molecular processes of viral infection. Portland Press Ltd. 2023-08-31 2023-08-10 /pmc/articles/PMC10578967/ /pubmed/37560901 http://dx.doi.org/10.1042/BST20230103 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY) (https://creativecommons.org/licenses/by/4.0/) . Open access for this article was enabled by the participation of University of Oxford in an all-inclusive Read & Publish agreement with Portland Press and the Biochemical Society under a transformative agreement with JISC.
spellingShingle Review Articles
Graham, Miles
Zhang, Peijun
Cryo-electron tomography to study viral infection
title Cryo-electron tomography to study viral infection
title_full Cryo-electron tomography to study viral infection
title_fullStr Cryo-electron tomography to study viral infection
title_full_unstemmed Cryo-electron tomography to study viral infection
title_short Cryo-electron tomography to study viral infection
title_sort cryo-electron tomography to study viral infection
topic Review Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10578967/
https://www.ncbi.nlm.nih.gov/pubmed/37560901
http://dx.doi.org/10.1042/BST20230103
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