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单细胞转录组鉴定阿尔茨海默病外周血生物标志物GZMK(+) CD8(+) T细胞
OBJECTIVE: Based on single-cell RNA sequencing (scRNA-seq) to explore immune characteristics in the peripheral blood of patients with Alzheimer's disease (AD) as biomarkers. METHODS: GSE168522, the scRNA-seq dataset of AD peripheral blood immune cells, was downloaded from the Gene Expression Om...
Formato: | Online Artículo Texto |
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Lenguaje: | English |
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四川大学学报(医学版)编辑部
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10579064/ https://www.ncbi.nlm.nih.gov/pubmed/37866940 http://dx.doi.org/10.12182/20230960107 |
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collection | PubMed |
description | OBJECTIVE: Based on single-cell RNA sequencing (scRNA-seq) to explore immune characteristics in the peripheral blood of patients with Alzheimer's disease (AD) as biomarkers. METHODS: GSE168522, the scRNA-seq dataset of AD peripheral blood immune cells, was downloaded from the Gene Expression Omnibus (GEO) database and was analyzed in the RAD-Blood web server (http://www.bioinform.cn/RAD-Blood/). The changes in blood cell composition in AD patients were analyzed. The abnormal communications between different types of cells in AD patients were investigated by the CellChat R package. RESULTS: There were two kinds of CD8(+) T cells in the blood of AD patients and healthy individuals, one of which highly expressed granzyme K (GZMK) (false discovery rate [FDR]<0.05), and the other highly expressed GZMA, GZMB, and GZMH (FDR<0.05). In the blood of AD patients, the content of GZMK(+) CD8(+) T cells was increased by 32.9% (P=5.15E-21), their interactions with other cell types were increased, and they might be associated with AD through the abnormal signal transduction of major histocompatibility complex class Ⅰ (MHC-Ⅰ). Erythrocyte provided the main ligands, that are, human leukocyte antigen (HLA) class Ⅰ molecules, including HLA-A, HLA-B, HLA-C, and HLA-E, for the abnormal MHC-Ⅰ signaling pathway of GZMK(+) CD8(+) T cells. The RESISTIN signaling pathway was specifically enriched in the blood of AD patients. CONCLUSION: The increased content of peripheral blood GZMK(+) CD8(+) T cells, the increased interaction between GZMK(+) CD8(+) T cells and erythrocytes, and the enhanced RESISTIN pathway are potential blood biomarkers of AD. |
format | Online Article Text |
id | pubmed-10579064 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | 四川大学学报(医学版)编辑部 |
record_format | MEDLINE/PubMed |
spelling | pubmed-105790642023-10-18 单细胞转录组鉴定阿尔茨海默病外周血生物标志物GZMK(+) CD8(+) T细胞 Sichuan Da Xue Xue Bao Yi Xue Ban 大数据与人工智能技术在生物医学多场景的应用 OBJECTIVE: Based on single-cell RNA sequencing (scRNA-seq) to explore immune characteristics in the peripheral blood of patients with Alzheimer's disease (AD) as biomarkers. METHODS: GSE168522, the scRNA-seq dataset of AD peripheral blood immune cells, was downloaded from the Gene Expression Omnibus (GEO) database and was analyzed in the RAD-Blood web server (http://www.bioinform.cn/RAD-Blood/). The changes in blood cell composition in AD patients were analyzed. The abnormal communications between different types of cells in AD patients were investigated by the CellChat R package. RESULTS: There were two kinds of CD8(+) T cells in the blood of AD patients and healthy individuals, one of which highly expressed granzyme K (GZMK) (false discovery rate [FDR]<0.05), and the other highly expressed GZMA, GZMB, and GZMH (FDR<0.05). In the blood of AD patients, the content of GZMK(+) CD8(+) T cells was increased by 32.9% (P=5.15E-21), their interactions with other cell types were increased, and they might be associated with AD through the abnormal signal transduction of major histocompatibility complex class Ⅰ (MHC-Ⅰ). Erythrocyte provided the main ligands, that are, human leukocyte antigen (HLA) class Ⅰ molecules, including HLA-A, HLA-B, HLA-C, and HLA-E, for the abnormal MHC-Ⅰ signaling pathway of GZMK(+) CD8(+) T cells. The RESISTIN signaling pathway was specifically enriched in the blood of AD patients. CONCLUSION: The increased content of peripheral blood GZMK(+) CD8(+) T cells, the increased interaction between GZMK(+) CD8(+) T cells and erythrocytes, and the enhanced RESISTIN pathway are potential blood biomarkers of AD. 四川大学学报(医学版)编辑部 2023-09-20 /pmc/articles/PMC10579064/ /pubmed/37866940 http://dx.doi.org/10.12182/20230960107 Text en © 2023《四川大学学报(医学版)》编辑部 版权所有 https://creativecommons.org/licenses/by-nc/4.0/开放获取 本文遵循知识共享署名—非商业性使用4.0国际许可协议(CC BY-NC 4.0),允许第三方对本刊发表的论文自由共享(即在任何媒介以任何形式复制、发行原文)、演绎(即修改、转换或以原文为基础进行创作),必须给出适当的署名,提供指向本文许可协议的链接,同时标明是否对原文作了修改;不得将本文用于商业目的。CC BY-NC 4.0许可协议访问 https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) https://creativecommons.org/licenses/by-nc/4.0/Open Access This article is licensed under a Creative Commons Attribution-NonCommercial 4.0 International license (CC BY-NC 4.0). In other words, the full-text content of the journal is made freely available for third-party users to copy and redistribute in any medium or format, and to remix, transform, and build upon the content of the journal. You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may not use the content of the journal for commercial purposes. For more information about the license, visit https://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) |
spellingShingle | 大数据与人工智能技术在生物医学多场景的应用 单细胞转录组鉴定阿尔茨海默病外周血生物标志物GZMK(+) CD8(+) T细胞 |
title | 单细胞转录组鉴定阿尔茨海默病外周血生物标志物GZMK(+) CD8(+) T细胞 |
title_full | 单细胞转录组鉴定阿尔茨海默病外周血生物标志物GZMK(+) CD8(+) T细胞 |
title_fullStr | 单细胞转录组鉴定阿尔茨海默病外周血生物标志物GZMK(+) CD8(+) T细胞 |
title_full_unstemmed | 单细胞转录组鉴定阿尔茨海默病外周血生物标志物GZMK(+) CD8(+) T细胞 |
title_short | 单细胞转录组鉴定阿尔茨海默病外周血生物标志物GZMK(+) CD8(+) T细胞 |
title_sort | 单细胞转录组鉴定阿尔茨海默病外周血生物标志物gzmk(+) cd8(+) t细胞 |
topic | 大数据与人工智能技术在生物医学多场景的应用 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10579064/ https://www.ncbi.nlm.nih.gov/pubmed/37866940 http://dx.doi.org/10.12182/20230960107 |
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