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Evaluation of concentration procedures, sample pre-treatment, and storage condition for the detection of SARS-CoV-2 in wastewater

Crucial information on the pandemic’s spread has been gathered by monitoring the trend of SARS-CoV-2 in wastewater. This surveillance has highlighted that the initial concentration is a critical step of the analytical procedure due to the low viral titer that may be present in this matrix. This pape...

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Autores principales: Cutrupi, Francesca, Rossi, Michele, Cadonna, Maria, Poznanski, Elisa, Manara, Serena, Postinghel, Mattia, Palumbi, Giulia, Bellisomi, Marta, Nicosia, Elena, Allaria, Giorgia, Dondero, Lorenzo, Veneri, Carolina, Mancini, Pamela, Ferraro, Giusy Bonanno, Rosa, Giuseppina, Suffredini, Elisabetta, Foladori, Paola, Grasselli, Elena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10579110/
https://www.ncbi.nlm.nih.gov/pubmed/37733200
http://dx.doi.org/10.1007/s11356-023-29696-y
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author Cutrupi, Francesca
Rossi, Michele
Cadonna, Maria
Poznanski, Elisa
Manara, Serena
Postinghel, Mattia
Palumbi, Giulia
Bellisomi, Marta
Nicosia, Elena
Allaria, Giorgia
Dondero, Lorenzo
Veneri, Carolina
Mancini, Pamela
Ferraro, Giusy Bonanno
Rosa, Giuseppina
Suffredini, Elisabetta
Foladori, Paola
Grasselli, Elena
author_facet Cutrupi, Francesca
Rossi, Michele
Cadonna, Maria
Poznanski, Elisa
Manara, Serena
Postinghel, Mattia
Palumbi, Giulia
Bellisomi, Marta
Nicosia, Elena
Allaria, Giorgia
Dondero, Lorenzo
Veneri, Carolina
Mancini, Pamela
Ferraro, Giusy Bonanno
Rosa, Giuseppina
Suffredini, Elisabetta
Foladori, Paola
Grasselli, Elena
author_sort Cutrupi, Francesca
collection PubMed
description Crucial information on the pandemic’s spread has been gathered by monitoring the trend of SARS-CoV-2 in wastewater. This surveillance has highlighted that the initial concentration is a critical step of the analytical procedure due to the low viral titer that may be present in this matrix. This paper presents the results of the evaluation of two different wastewater concentration protocols to determine the most efficient and cost-effective. The two methods tested were the following: (a) a biphasic separation system with PEG-dextran and (b) a PEG/NaCl precipitation protocol. Other aspects of the detection method were also investigated including the influence of storage temperature on virus recovery and the heat treatment of pasteurization, which aims to make samples safer for operators and the environment. The PEG/NaCl precipitation method was found to perform better than the biphasic separation system, allowing for more sensitive identification of the presence of the virus and the detection of a higher viral titer than that identified with the biphasic separation in all results. Storage of the samples at 4.3±0.2°C for up to 3 weeks did not adversely affect the virus titer and the pasteurization pre-treatment increases operator safety and maintains the identification of the viral concentration. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11356-023-29696-y.
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spelling pubmed-105791102023-10-18 Evaluation of concentration procedures, sample pre-treatment, and storage condition for the detection of SARS-CoV-2 in wastewater Cutrupi, Francesca Rossi, Michele Cadonna, Maria Poznanski, Elisa Manara, Serena Postinghel, Mattia Palumbi, Giulia Bellisomi, Marta Nicosia, Elena Allaria, Giorgia Dondero, Lorenzo Veneri, Carolina Mancini, Pamela Ferraro, Giusy Bonanno Rosa, Giuseppina Suffredini, Elisabetta Foladori, Paola Grasselli, Elena Environ Sci Pollut Res Int Research Article Crucial information on the pandemic’s spread has been gathered by monitoring the trend of SARS-CoV-2 in wastewater. This surveillance has highlighted that the initial concentration is a critical step of the analytical procedure due to the low viral titer that may be present in this matrix. This paper presents the results of the evaluation of two different wastewater concentration protocols to determine the most efficient and cost-effective. The two methods tested were the following: (a) a biphasic separation system with PEG-dextran and (b) a PEG/NaCl precipitation protocol. Other aspects of the detection method were also investigated including the influence of storage temperature on virus recovery and the heat treatment of pasteurization, which aims to make samples safer for operators and the environment. The PEG/NaCl precipitation method was found to perform better than the biphasic separation system, allowing for more sensitive identification of the presence of the virus and the detection of a higher viral titer than that identified with the biphasic separation in all results. Storage of the samples at 4.3±0.2°C for up to 3 weeks did not adversely affect the virus titer and the pasteurization pre-treatment increases operator safety and maintains the identification of the viral concentration. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11356-023-29696-y. Springer Berlin Heidelberg 2023-09-21 2023 /pmc/articles/PMC10579110/ /pubmed/37733200 http://dx.doi.org/10.1007/s11356-023-29696-y Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Cutrupi, Francesca
Rossi, Michele
Cadonna, Maria
Poznanski, Elisa
Manara, Serena
Postinghel, Mattia
Palumbi, Giulia
Bellisomi, Marta
Nicosia, Elena
Allaria, Giorgia
Dondero, Lorenzo
Veneri, Carolina
Mancini, Pamela
Ferraro, Giusy Bonanno
Rosa, Giuseppina
Suffredini, Elisabetta
Foladori, Paola
Grasselli, Elena
Evaluation of concentration procedures, sample pre-treatment, and storage condition for the detection of SARS-CoV-2 in wastewater
title Evaluation of concentration procedures, sample pre-treatment, and storage condition for the detection of SARS-CoV-2 in wastewater
title_full Evaluation of concentration procedures, sample pre-treatment, and storage condition for the detection of SARS-CoV-2 in wastewater
title_fullStr Evaluation of concentration procedures, sample pre-treatment, and storage condition for the detection of SARS-CoV-2 in wastewater
title_full_unstemmed Evaluation of concentration procedures, sample pre-treatment, and storage condition for the detection of SARS-CoV-2 in wastewater
title_short Evaluation of concentration procedures, sample pre-treatment, and storage condition for the detection of SARS-CoV-2 in wastewater
title_sort evaluation of concentration procedures, sample pre-treatment, and storage condition for the detection of sars-cov-2 in wastewater
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10579110/
https://www.ncbi.nlm.nih.gov/pubmed/37733200
http://dx.doi.org/10.1007/s11356-023-29696-y
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