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Distinct pathways for export of silencing RNA in Caenorhabditis elegans systemic RNAi
Dietary supplied double-stranded RNA (dsRNA) can trigger RNA interference (RNAi) systemically in some animals, including the nematode Caenorhabditis elegans. Although this phenomenon has been utilized as a major tool for gene silencing in C. elegans, how cells spread the silencing RNA throughout the...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10579535/ https://www.ncbi.nlm.nih.gov/pubmed/37854694 http://dx.doi.org/10.1016/j.isci.2023.108067 |
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author | Yoshida, Keita Suehiro, Yuji Dejima, Katsufumi Yoshina, Sawako Mitani, Shohei |
author_facet | Yoshida, Keita Suehiro, Yuji Dejima, Katsufumi Yoshina, Sawako Mitani, Shohei |
author_sort | Yoshida, Keita |
collection | PubMed |
description | Dietary supplied double-stranded RNA (dsRNA) can trigger RNA interference (RNAi) systemically in some animals, including the nematode Caenorhabditis elegans. Although this phenomenon has been utilized as a major tool for gene silencing in C. elegans, how cells spread the silencing RNA throughout the organism is largely unknown. Here, we identify two novel systemic RNAi-related factors, REXD-1 and TBC-3, and show that these two factors together with SID-5 act redundantly to promote systemic spreading of dsRNA. Animals that are defective in all REXD-1, TBC-3, and SID-5 functions show strong deficiency in export of dsRNA from intestinal cells, whereas cellular uptake and processing of dsRNA and general secretion events other than dsRNA secretion are still functional in the triple mutant animals. Our findings reveal pathways that specifically regulate the export of dsRNA in parallel, implying the importance of spreading RNA molecules for intercellular communication in organisms. |
format | Online Article Text |
id | pubmed-10579535 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-105795352023-10-18 Distinct pathways for export of silencing RNA in Caenorhabditis elegans systemic RNAi Yoshida, Keita Suehiro, Yuji Dejima, Katsufumi Yoshina, Sawako Mitani, Shohei iScience Article Dietary supplied double-stranded RNA (dsRNA) can trigger RNA interference (RNAi) systemically in some animals, including the nematode Caenorhabditis elegans. Although this phenomenon has been utilized as a major tool for gene silencing in C. elegans, how cells spread the silencing RNA throughout the organism is largely unknown. Here, we identify two novel systemic RNAi-related factors, REXD-1 and TBC-3, and show that these two factors together with SID-5 act redundantly to promote systemic spreading of dsRNA. Animals that are defective in all REXD-1, TBC-3, and SID-5 functions show strong deficiency in export of dsRNA from intestinal cells, whereas cellular uptake and processing of dsRNA and general secretion events other than dsRNA secretion are still functional in the triple mutant animals. Our findings reveal pathways that specifically regulate the export of dsRNA in parallel, implying the importance of spreading RNA molecules for intercellular communication in organisms. Elsevier 2023-09-28 /pmc/articles/PMC10579535/ /pubmed/37854694 http://dx.doi.org/10.1016/j.isci.2023.108067 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Yoshida, Keita Suehiro, Yuji Dejima, Katsufumi Yoshina, Sawako Mitani, Shohei Distinct pathways for export of silencing RNA in Caenorhabditis elegans systemic RNAi |
title | Distinct pathways for export of silencing RNA in Caenorhabditis elegans systemic RNAi |
title_full | Distinct pathways for export of silencing RNA in Caenorhabditis elegans systemic RNAi |
title_fullStr | Distinct pathways for export of silencing RNA in Caenorhabditis elegans systemic RNAi |
title_full_unstemmed | Distinct pathways for export of silencing RNA in Caenorhabditis elegans systemic RNAi |
title_short | Distinct pathways for export of silencing RNA in Caenorhabditis elegans systemic RNAi |
title_sort | distinct pathways for export of silencing rna in caenorhabditis elegans systemic rnai |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10579535/ https://www.ncbi.nlm.nih.gov/pubmed/37854694 http://dx.doi.org/10.1016/j.isci.2023.108067 |
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