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Gut microbiota–bile acid‐vitamin D axis plays an important role in determining oocyte quality and embryonic development
OBJECTIVE: To reveal whether gut microbiota and their metabolites are correlated with oocyte quality decline caused by circadian rhythm disruption, and to search possible approaches for improving oocyte quality. DESIGN: A mouse model exposed to continuous light was established. The oocyte quality, e...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10580005/ https://www.ncbi.nlm.nih.gov/pubmed/37846137 http://dx.doi.org/10.1002/ctm2.1236 |
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author | Li, Ang Li, Fei Song, Wei Lei, Zi‐Li Sha, Qian‐Qian Liu, Shao‐Yuan Zhou, Chang‐Yin Zhang, Xue Li, Xiao‐Zhen Schatten, Heide Zhang, Teng Sun, Qing‐Yuan Ou, Xiang‐Hong |
author_facet | Li, Ang Li, Fei Song, Wei Lei, Zi‐Li Sha, Qian‐Qian Liu, Shao‐Yuan Zhou, Chang‐Yin Zhang, Xue Li, Xiao‐Zhen Schatten, Heide Zhang, Teng Sun, Qing‐Yuan Ou, Xiang‐Hong |
author_sort | Li, Ang |
collection | PubMed |
description | OBJECTIVE: To reveal whether gut microbiota and their metabolites are correlated with oocyte quality decline caused by circadian rhythm disruption, and to search possible approaches for improving oocyte quality. DESIGN: A mouse model exposed to continuous light was established. The oocyte quality, embryonic development, microbial metabolites and gut microbiota were analyzed. Intragastric administration of microbial metabolites was conducted to confirm the relationship between gut microbiota and oocyte quality and embryonic development. RESULTS: Firstly, we found that oocyte quality and embryonic development decreased in mice exposed to continuous light. Through metabolomics profiling and 16S rDNA‐seq, we found that the intestinal absorption capacity of vitamin D was decreased due to significant decrease of bile acids such as lithocholic acid (LCA), which was significantly associated with increased abundance of Turicibacter. Subsequently, the concentrations of anti‐Mullerian hormone (AMH) hormone in blood and melatonin in follicular fluid were reduced, which is the main reason for the decline of oocyte quality and early embryonic development, and this was rescued by injection of vitamin D3 (VD3). Secondly, melatonin rescued oocyte quality and embryonic development by increasing the concentration of lithocholic acid and reducing the concentration of oxidative stress metabolites in the intestine. Thirdly, we found six metabolites that could rescue oocyte quality and early embryonic development, among which LCA of 30 mg/kg and NorDCA of 15 mg/kg had the best rescue effect. CONCLUSION: These findings confirm the link between ovarian function and gut microbiota regulation by microbial metabolites and have potential value for improving ovary function. |
format | Online Article Text |
id | pubmed-10580005 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-105800052023-10-18 Gut microbiota–bile acid‐vitamin D axis plays an important role in determining oocyte quality and embryonic development Li, Ang Li, Fei Song, Wei Lei, Zi‐Li Sha, Qian‐Qian Liu, Shao‐Yuan Zhou, Chang‐Yin Zhang, Xue Li, Xiao‐Zhen Schatten, Heide Zhang, Teng Sun, Qing‐Yuan Ou, Xiang‐Hong Clin Transl Med Research Articles OBJECTIVE: To reveal whether gut microbiota and their metabolites are correlated with oocyte quality decline caused by circadian rhythm disruption, and to search possible approaches for improving oocyte quality. DESIGN: A mouse model exposed to continuous light was established. The oocyte quality, embryonic development, microbial metabolites and gut microbiota were analyzed. Intragastric administration of microbial metabolites was conducted to confirm the relationship between gut microbiota and oocyte quality and embryonic development. RESULTS: Firstly, we found that oocyte quality and embryonic development decreased in mice exposed to continuous light. Through metabolomics profiling and 16S rDNA‐seq, we found that the intestinal absorption capacity of vitamin D was decreased due to significant decrease of bile acids such as lithocholic acid (LCA), which was significantly associated with increased abundance of Turicibacter. Subsequently, the concentrations of anti‐Mullerian hormone (AMH) hormone in blood and melatonin in follicular fluid were reduced, which is the main reason for the decline of oocyte quality and early embryonic development, and this was rescued by injection of vitamin D3 (VD3). Secondly, melatonin rescued oocyte quality and embryonic development by increasing the concentration of lithocholic acid and reducing the concentration of oxidative stress metabolites in the intestine. Thirdly, we found six metabolites that could rescue oocyte quality and early embryonic development, among which LCA of 30 mg/kg and NorDCA of 15 mg/kg had the best rescue effect. CONCLUSION: These findings confirm the link between ovarian function and gut microbiota regulation by microbial metabolites and have potential value for improving ovary function. John Wiley and Sons Inc. 2023-10-17 /pmc/articles/PMC10580005/ /pubmed/37846137 http://dx.doi.org/10.1002/ctm2.1236 Text en © 2023 The Authors. Clinical and Translational Medicine published by John Wiley & Sons Australia, Ltd on behalf of Shanghai Institute of Clinical Bioinformatics. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Li, Ang Li, Fei Song, Wei Lei, Zi‐Li Sha, Qian‐Qian Liu, Shao‐Yuan Zhou, Chang‐Yin Zhang, Xue Li, Xiao‐Zhen Schatten, Heide Zhang, Teng Sun, Qing‐Yuan Ou, Xiang‐Hong Gut microbiota–bile acid‐vitamin D axis plays an important role in determining oocyte quality and embryonic development |
title | Gut microbiota–bile acid‐vitamin D axis plays an important role in determining oocyte quality and embryonic development |
title_full | Gut microbiota–bile acid‐vitamin D axis plays an important role in determining oocyte quality and embryonic development |
title_fullStr | Gut microbiota–bile acid‐vitamin D axis plays an important role in determining oocyte quality and embryonic development |
title_full_unstemmed | Gut microbiota–bile acid‐vitamin D axis plays an important role in determining oocyte quality and embryonic development |
title_short | Gut microbiota–bile acid‐vitamin D axis plays an important role in determining oocyte quality and embryonic development |
title_sort | gut microbiota–bile acid‐vitamin d axis plays an important role in determining oocyte quality and embryonic development |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10580005/ https://www.ncbi.nlm.nih.gov/pubmed/37846137 http://dx.doi.org/10.1002/ctm2.1236 |
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