Cargando…
Neuropathic pain following spinal cord hemisection induced by the reorganization in primary somatosensory cortex and regulated by neuronal activity of lateral parabrachial nucleus
AIMS: Neuropathic pain after spinal cord injury (SCI) remains a common and thorny problem, influencing the life quality severely. This study aimed to elucidate the reorganization of the primary sensory cortex (S1) and the regulatory mechanism of the lateral parabrachial nucleus (lPBN) in the presenc...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10580357/ https://www.ncbi.nlm.nih.gov/pubmed/37170721 http://dx.doi.org/10.1111/cns.14258 |
_version_ | 1785121926630342656 |
---|---|
author | Li, Jing Tian, Chao Yuan, Shiyang Yin, Zhenyu Wei, Liangpeng Chen, Feng Dong, Xi Liu, Aili Wang, Zhenhuan Wu, Tongrui Tian, Chunxiao Niu, Lin Wang, Lei Wang, Pu Xie, Wanyu Cao, Fujiang Shen, Hui |
author_facet | Li, Jing Tian, Chao Yuan, Shiyang Yin, Zhenyu Wei, Liangpeng Chen, Feng Dong, Xi Liu, Aili Wang, Zhenhuan Wu, Tongrui Tian, Chunxiao Niu, Lin Wang, Lei Wang, Pu Xie, Wanyu Cao, Fujiang Shen, Hui |
author_sort | Li, Jing |
collection | PubMed |
description | AIMS: Neuropathic pain after spinal cord injury (SCI) remains a common and thorny problem, influencing the life quality severely. This study aimed to elucidate the reorganization of the primary sensory cortex (S1) and the regulatory mechanism of the lateral parabrachial nucleus (lPBN) in the presence of allodynia or hyperalgesia after left spinal cord hemisection injury (LHS). METHODS: Through behavioral tests, we first identified mechanical allodynia and thermal hyperalgesia following LHS. We then applied two‐photon microscopy to observe calcium activity in S1 during mechanical or thermal stimulation and long‐term spontaneous calcium activity after LHS. By slice patch clamp recording, the electrophysiological characteristics of neurons in lPBN were explored. Finally, exploiting chemogenetic activation or inhibition of the neurons in lPBN, allodynia or hyperalgesia was regulated. RESULTS: The calcium activity in left S1 was increased during mechanical stimulation of right hind limb and thermal stimulation of tail, whereas in right S1 it was increased only with thermal stimulation of tail. The spontaneous calcium activity in right S1 changed more dramatically than that in left S1 after LHS. The lPBN was also activated after LHS, and exploiting chemogenetic activation or inhibition of the neurons in lPBN could induce or alleviate allodynia and hyperalgesia in central neuropathic pain. CONCLUSION: The neuronal activity changes in S1 are closely related to limb pain, which has accurate anatomical correspondence. After LHS, the spontaneously increased functional connectivity of calcium transient in left S1 is likely causing the mechanical allodynia in right hind limb and increased neuronal activity in bilateral S1 may induce thermal hyperalgesia in tail. This state of allodynia and hyperalgesia can be regulated by lPBN. |
format | Online Article Text |
id | pubmed-10580357 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-105803572023-10-18 Neuropathic pain following spinal cord hemisection induced by the reorganization in primary somatosensory cortex and regulated by neuronal activity of lateral parabrachial nucleus Li, Jing Tian, Chao Yuan, Shiyang Yin, Zhenyu Wei, Liangpeng Chen, Feng Dong, Xi Liu, Aili Wang, Zhenhuan Wu, Tongrui Tian, Chunxiao Niu, Lin Wang, Lei Wang, Pu Xie, Wanyu Cao, Fujiang Shen, Hui CNS Neurosci Ther Original Articles AIMS: Neuropathic pain after spinal cord injury (SCI) remains a common and thorny problem, influencing the life quality severely. This study aimed to elucidate the reorganization of the primary sensory cortex (S1) and the regulatory mechanism of the lateral parabrachial nucleus (lPBN) in the presence of allodynia or hyperalgesia after left spinal cord hemisection injury (LHS). METHODS: Through behavioral tests, we first identified mechanical allodynia and thermal hyperalgesia following LHS. We then applied two‐photon microscopy to observe calcium activity in S1 during mechanical or thermal stimulation and long‐term spontaneous calcium activity after LHS. By slice patch clamp recording, the electrophysiological characteristics of neurons in lPBN were explored. Finally, exploiting chemogenetic activation or inhibition of the neurons in lPBN, allodynia or hyperalgesia was regulated. RESULTS: The calcium activity in left S1 was increased during mechanical stimulation of right hind limb and thermal stimulation of tail, whereas in right S1 it was increased only with thermal stimulation of tail. The spontaneous calcium activity in right S1 changed more dramatically than that in left S1 after LHS. The lPBN was also activated after LHS, and exploiting chemogenetic activation or inhibition of the neurons in lPBN could induce or alleviate allodynia and hyperalgesia in central neuropathic pain. CONCLUSION: The neuronal activity changes in S1 are closely related to limb pain, which has accurate anatomical correspondence. After LHS, the spontaneously increased functional connectivity of calcium transient in left S1 is likely causing the mechanical allodynia in right hind limb and increased neuronal activity in bilateral S1 may induce thermal hyperalgesia in tail. This state of allodynia and hyperalgesia can be regulated by lPBN. John Wiley and Sons Inc. 2023-05-11 /pmc/articles/PMC10580357/ /pubmed/37170721 http://dx.doi.org/10.1111/cns.14258 Text en © 2023 The Authors. CNS Neuroscience & Therapeutics published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles Li, Jing Tian, Chao Yuan, Shiyang Yin, Zhenyu Wei, Liangpeng Chen, Feng Dong, Xi Liu, Aili Wang, Zhenhuan Wu, Tongrui Tian, Chunxiao Niu, Lin Wang, Lei Wang, Pu Xie, Wanyu Cao, Fujiang Shen, Hui Neuropathic pain following spinal cord hemisection induced by the reorganization in primary somatosensory cortex and regulated by neuronal activity of lateral parabrachial nucleus |
title | Neuropathic pain following spinal cord hemisection induced by the reorganization in primary somatosensory cortex and regulated by neuronal activity of lateral parabrachial nucleus |
title_full | Neuropathic pain following spinal cord hemisection induced by the reorganization in primary somatosensory cortex and regulated by neuronal activity of lateral parabrachial nucleus |
title_fullStr | Neuropathic pain following spinal cord hemisection induced by the reorganization in primary somatosensory cortex and regulated by neuronal activity of lateral parabrachial nucleus |
title_full_unstemmed | Neuropathic pain following spinal cord hemisection induced by the reorganization in primary somatosensory cortex and regulated by neuronal activity of lateral parabrachial nucleus |
title_short | Neuropathic pain following spinal cord hemisection induced by the reorganization in primary somatosensory cortex and regulated by neuronal activity of lateral parabrachial nucleus |
title_sort | neuropathic pain following spinal cord hemisection induced by the reorganization in primary somatosensory cortex and regulated by neuronal activity of lateral parabrachial nucleus |
topic | Original Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10580357/ https://www.ncbi.nlm.nih.gov/pubmed/37170721 http://dx.doi.org/10.1111/cns.14258 |
work_keys_str_mv | AT lijing neuropathicpainfollowingspinalcordhemisectioninducedbythereorganizationinprimarysomatosensorycortexandregulatedbyneuronalactivityoflateralparabrachialnucleus AT tianchao neuropathicpainfollowingspinalcordhemisectioninducedbythereorganizationinprimarysomatosensorycortexandregulatedbyneuronalactivityoflateralparabrachialnucleus AT yuanshiyang neuropathicpainfollowingspinalcordhemisectioninducedbythereorganizationinprimarysomatosensorycortexandregulatedbyneuronalactivityoflateralparabrachialnucleus AT yinzhenyu neuropathicpainfollowingspinalcordhemisectioninducedbythereorganizationinprimarysomatosensorycortexandregulatedbyneuronalactivityoflateralparabrachialnucleus AT weiliangpeng neuropathicpainfollowingspinalcordhemisectioninducedbythereorganizationinprimarysomatosensorycortexandregulatedbyneuronalactivityoflateralparabrachialnucleus AT chenfeng neuropathicpainfollowingspinalcordhemisectioninducedbythereorganizationinprimarysomatosensorycortexandregulatedbyneuronalactivityoflateralparabrachialnucleus AT dongxi neuropathicpainfollowingspinalcordhemisectioninducedbythereorganizationinprimarysomatosensorycortexandregulatedbyneuronalactivityoflateralparabrachialnucleus AT liuaili neuropathicpainfollowingspinalcordhemisectioninducedbythereorganizationinprimarysomatosensorycortexandregulatedbyneuronalactivityoflateralparabrachialnucleus AT wangzhenhuan neuropathicpainfollowingspinalcordhemisectioninducedbythereorganizationinprimarysomatosensorycortexandregulatedbyneuronalactivityoflateralparabrachialnucleus AT wutongrui neuropathicpainfollowingspinalcordhemisectioninducedbythereorganizationinprimarysomatosensorycortexandregulatedbyneuronalactivityoflateralparabrachialnucleus AT tianchunxiao neuropathicpainfollowingspinalcordhemisectioninducedbythereorganizationinprimarysomatosensorycortexandregulatedbyneuronalactivityoflateralparabrachialnucleus AT niulin neuropathicpainfollowingspinalcordhemisectioninducedbythereorganizationinprimarysomatosensorycortexandregulatedbyneuronalactivityoflateralparabrachialnucleus AT wanglei neuropathicpainfollowingspinalcordhemisectioninducedbythereorganizationinprimarysomatosensorycortexandregulatedbyneuronalactivityoflateralparabrachialnucleus AT wangpu neuropathicpainfollowingspinalcordhemisectioninducedbythereorganizationinprimarysomatosensorycortexandregulatedbyneuronalactivityoflateralparabrachialnucleus AT xiewanyu neuropathicpainfollowingspinalcordhemisectioninducedbythereorganizationinprimarysomatosensorycortexandregulatedbyneuronalactivityoflateralparabrachialnucleus AT caofujiang neuropathicpainfollowingspinalcordhemisectioninducedbythereorganizationinprimarysomatosensorycortexandregulatedbyneuronalactivityoflateralparabrachialnucleus AT shenhui neuropathicpainfollowingspinalcordhemisectioninducedbythereorganizationinprimarysomatosensorycortexandregulatedbyneuronalactivityoflateralparabrachialnucleus |