Cargando…

Molecular identification of carnivore chaphamaparvovirus 2 (feline chaphamaparvovirus) in cats with diarrhea from China

Chaphamaparvovirus carnivoran2 (feline chaphamaparvovirus, FeChPV) is a novel feline parvovirus originally detected in Canadian cats in 2019, and it has also been identified in domestic cats in other nations. To evaluate the prevalence and genetic diversity of FeChPV in China, rectal swabs of pet ca...

Descripción completa

Detalles Bibliográficos
Autores principales: Cui, Hao, Zhang, Zhibin, Xu, Xin, Zuo, Kejing, Ji, Jun, Guo, Ge, Kan, Yunchao, Yao, Lunguang, Xie, Qingmei, Bi, Yingzuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10580804/
https://www.ncbi.nlm.nih.gov/pubmed/37854096
http://dx.doi.org/10.3389/fvets.2023.1252628
_version_ 1785122014321704960
author Cui, Hao
Zhang, Zhibin
Xu, Xin
Zuo, Kejing
Ji, Jun
Guo, Ge
Kan, Yunchao
Yao, Lunguang
Xie, Qingmei
Bi, Yingzuo
author_facet Cui, Hao
Zhang, Zhibin
Xu, Xin
Zuo, Kejing
Ji, Jun
Guo, Ge
Kan, Yunchao
Yao, Lunguang
Xie, Qingmei
Bi, Yingzuo
author_sort Cui, Hao
collection PubMed
description Chaphamaparvovirus carnivoran2 (feline chaphamaparvovirus, FeChPV) is a novel feline parvovirus originally detected in Canadian cats in 2019, and it has also been identified in domestic cats in other nations. To evaluate the prevalence and genetic diversity of FeChPV in China, rectal swabs of pet cats from Henan, Guangdong, Anhui, Zhejiang, and Inner Mongolia provinces were collected. Of the 230 samples subjected to nested polymerase chain reaction, 6 (2.6%) tested positive for FeChPV. Although all positive samples were from cats with diarrhea, statistical analyses revealed no correlation between the presence of the virus and clinical symptoms (p > 0.05). Phylogenetic trees of nonstructural protein 1 (NS1) and capsid protein (VP1) demonstrated that these six new strains formed a major branch with other reference FeChPV strains and considerably differed from Chaphamaparvoviru carnivoran1. Moreover, recombination analysis revealed that the FeChPV strain CHN20201025, previously detected in a dog, was a recombinant and strains CHN200228 and CHN180917, identified in this study, were the closest relatives to the parental strains. The findings of this study and a previous study wherein FeChPV was detected in dogs suggest that FeChPV can propagate between species. Additionally, these findings indicate that the genetic diversity of FeChPV can provide an insight into the epidemiological status of FeChPV in China.
format Online
Article
Text
id pubmed-10580804
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-105808042023-10-18 Molecular identification of carnivore chaphamaparvovirus 2 (feline chaphamaparvovirus) in cats with diarrhea from China Cui, Hao Zhang, Zhibin Xu, Xin Zuo, Kejing Ji, Jun Guo, Ge Kan, Yunchao Yao, Lunguang Xie, Qingmei Bi, Yingzuo Front Vet Sci Veterinary Science Chaphamaparvovirus carnivoran2 (feline chaphamaparvovirus, FeChPV) is a novel feline parvovirus originally detected in Canadian cats in 2019, and it has also been identified in domestic cats in other nations. To evaluate the prevalence and genetic diversity of FeChPV in China, rectal swabs of pet cats from Henan, Guangdong, Anhui, Zhejiang, and Inner Mongolia provinces were collected. Of the 230 samples subjected to nested polymerase chain reaction, 6 (2.6%) tested positive for FeChPV. Although all positive samples were from cats with diarrhea, statistical analyses revealed no correlation between the presence of the virus and clinical symptoms (p > 0.05). Phylogenetic trees of nonstructural protein 1 (NS1) and capsid protein (VP1) demonstrated that these six new strains formed a major branch with other reference FeChPV strains and considerably differed from Chaphamaparvoviru carnivoran1. Moreover, recombination analysis revealed that the FeChPV strain CHN20201025, previously detected in a dog, was a recombinant and strains CHN200228 and CHN180917, identified in this study, were the closest relatives to the parental strains. The findings of this study and a previous study wherein FeChPV was detected in dogs suggest that FeChPV can propagate between species. Additionally, these findings indicate that the genetic diversity of FeChPV can provide an insight into the epidemiological status of FeChPV in China. Frontiers Media S.A. 2023-10-03 /pmc/articles/PMC10580804/ /pubmed/37854096 http://dx.doi.org/10.3389/fvets.2023.1252628 Text en Copyright © 2023 Cui, Zhang, Xu, Zuo, Ji, Guo, Kan, Yao, Xie and Bi. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Cui, Hao
Zhang, Zhibin
Xu, Xin
Zuo, Kejing
Ji, Jun
Guo, Ge
Kan, Yunchao
Yao, Lunguang
Xie, Qingmei
Bi, Yingzuo
Molecular identification of carnivore chaphamaparvovirus 2 (feline chaphamaparvovirus) in cats with diarrhea from China
title Molecular identification of carnivore chaphamaparvovirus 2 (feline chaphamaparvovirus) in cats with diarrhea from China
title_full Molecular identification of carnivore chaphamaparvovirus 2 (feline chaphamaparvovirus) in cats with diarrhea from China
title_fullStr Molecular identification of carnivore chaphamaparvovirus 2 (feline chaphamaparvovirus) in cats with diarrhea from China
title_full_unstemmed Molecular identification of carnivore chaphamaparvovirus 2 (feline chaphamaparvovirus) in cats with diarrhea from China
title_short Molecular identification of carnivore chaphamaparvovirus 2 (feline chaphamaparvovirus) in cats with diarrhea from China
title_sort molecular identification of carnivore chaphamaparvovirus 2 (feline chaphamaparvovirus) in cats with diarrhea from china
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10580804/
https://www.ncbi.nlm.nih.gov/pubmed/37854096
http://dx.doi.org/10.3389/fvets.2023.1252628
work_keys_str_mv AT cuihao molecularidentificationofcarnivorechaphamaparvovirus2felinechaphamaparvovirusincatswithdiarrheafromchina
AT zhangzhibin molecularidentificationofcarnivorechaphamaparvovirus2felinechaphamaparvovirusincatswithdiarrheafromchina
AT xuxin molecularidentificationofcarnivorechaphamaparvovirus2felinechaphamaparvovirusincatswithdiarrheafromchina
AT zuokejing molecularidentificationofcarnivorechaphamaparvovirus2felinechaphamaparvovirusincatswithdiarrheafromchina
AT jijun molecularidentificationofcarnivorechaphamaparvovirus2felinechaphamaparvovirusincatswithdiarrheafromchina
AT guoge molecularidentificationofcarnivorechaphamaparvovirus2felinechaphamaparvovirusincatswithdiarrheafromchina
AT kanyunchao molecularidentificationofcarnivorechaphamaparvovirus2felinechaphamaparvovirusincatswithdiarrheafromchina
AT yaolunguang molecularidentificationofcarnivorechaphamaparvovirus2felinechaphamaparvovirusincatswithdiarrheafromchina
AT xieqingmei molecularidentificationofcarnivorechaphamaparvovirus2felinechaphamaparvovirusincatswithdiarrheafromchina
AT biyingzuo molecularidentificationofcarnivorechaphamaparvovirus2felinechaphamaparvovirusincatswithdiarrheafromchina