A qPCR Method to Assay Endonuclease Activity of Cas9-sgRNA Ribonucleoprotein Complexes

The CRISPR-Cas system has emerged as the most efficient genome editing technique for a wide range of cells. Delivery of the Cas9-sgRNA ribonucleoprotein complex (Cas9 RNP) has gained popularity. The objective of this study was to develop a quantitative polymerase chain reaction (qPCR)-based assay to...

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Detalles Bibliográficos
Autores principales: Nguyen, Minh Tri, Kim, Seul-Ah, Cheng, Ya-Yun, Hong, Sung Hoon, Jin, Yong-Su, Han, Nam Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society for Microbiology and Biotechnology 2023
Materias:
Research article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10580886/
https://www.ncbi.nlm.nih.gov/pubmed/37415091
http://dx.doi.org/10.4014/jmb.2305.05010

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10580886/
https://www.ncbi.nlm.nih.gov/pubmed/37415091
http://dx.doi.org/10.4014/jmb.2305.05010

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