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Improved transformation efficiency in Mycoplasma hominis enables disruption of the MIB–MIP system targeting human immunoglobulins

The pathogenicity of Mycoplasma hominis is poorly understood, mainly due to the absence of efficient genetic tools. A polyethylene glycol-mediated transformation protocol was recently developed for the M. hominis reference strain M132 using the pMT85-Tet plasmid. The transformation efficiency remain...

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Detalles Bibliográficos
Autores principales:	Guiraud, Jennifer, Le Roy, Chloé, Rideau, Fabien, Sirand-Pugnet, Pascal, Lartigue, Carole, Bébéar, Cécile, Arfi, Yonathan, Pereyre, Sabine
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2023
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581049/ https://www.ncbi.nlm.nih.gov/pubmed/37737635 http://dx.doi.org/10.1128/spectrum.01873-23

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