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Optimization of real-time PCR protocols from lymph node bovine tissue for direct detection of Mycobacterium tuberculosis complex

Bovine tuberculosis (bTB) is a zoonotic disease and a global health problem that is subjected to obligatory eradication programs in the European Union. Microbiological culture is an imperfect technique for bTB diagnosis. This study aims to compare and validate two DNA isolation protocols and three d...

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Detalles Bibliográficos
Autores principales:	Vera-Salmoral, Eduardo, Gómez-Laguna, Jaime, Galán-Relaño, Ángela, Ruedas-Torres, Inés, Carrasco, Librado, Luque, Inmaculada, Huerta, Belén, Sánchez-Carvajal, José María
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Microbiology 2023
Materias:	Methods and Protocols
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581224/ https://www.ncbi.nlm.nih.gov/pubmed/37707455 http://dx.doi.org/10.1128/spectrum.00348-23

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