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Serotype distribution and antibiogram of Streptococcus parauberis isolated from fish in South Korea

Streptococcus parauberis is the dominant etiological agent of streptococcosis, the most devastating bacterial disease in the olive flounder farming industry in South Korea. In this study, the distribution of serotypes, antimicrobial susceptibility, and presence of antimicrobial resistance genes (ARG...

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Autores principales: Lee, Yoonhang, Kim, Nameun, Roh, HeyongJin, Ho, Diem Tho, Park, Jiyeon, Lee, Ju Yeop, Kim, Yoon-Jae, Kang, Hyo-Young, Lee, Jungmin, Song, Jun-Young, Kim, Ahran, Kim, Myoung Sug, Cho, Miyoung, Choi, Hye Sung, Park, Chan-Il, Kim, Do-Hyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581249/
https://www.ncbi.nlm.nih.gov/pubmed/37555676
http://dx.doi.org/10.1128/spectrum.04400-22
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author Lee, Yoonhang
Kim, Nameun
Roh, HeyongJin
Ho, Diem Tho
Park, Jiyeon
Lee, Ju Yeop
Kim, Yoon-Jae
Kang, Hyo-Young
Lee, Jungmin
Song, Jun-Young
Kim, Ahran
Kim, Myoung Sug
Cho, Miyoung
Choi, Hye Sung
Park, Chan-Il
Kim, Do-Hyung
author_facet Lee, Yoonhang
Kim, Nameun
Roh, HeyongJin
Ho, Diem Tho
Park, Jiyeon
Lee, Ju Yeop
Kim, Yoon-Jae
Kang, Hyo-Young
Lee, Jungmin
Song, Jun-Young
Kim, Ahran
Kim, Myoung Sug
Cho, Miyoung
Choi, Hye Sung
Park, Chan-Il
Kim, Do-Hyung
author_sort Lee, Yoonhang
collection PubMed
description Streptococcus parauberis is the dominant etiological agent of streptococcosis, the most devastating bacterial disease in the olive flounder farming industry in South Korea. In this study, the distribution of serotypes, antimicrobial susceptibility, and presence of antimicrobial resistance genes (ARGs) in S. parauberis isolates obtained between 1999 and 2021 was thoroughly investigated to gain insight into the dynamics of their presence and the relationship between serotypes and antimicrobial resistance. Disk diffusion testing of 103 isolates against 10 antimicrobial agents was performed, and epidemiological cut-off values generated through normalized resistance interpretation analysis were used to classify wild-type (WT) and non-wild-type (NWT) populations. Principal component analysis and hierarchical clustering were implemented to achieve an understanding on the relationship between serotypes and antimicrobial resistance patterns. PCR-based serotyping showed that serotype Ia (67.1%) was the most prevalent in South Korea, followed by serotypes Ib/Ic (25.2%) and II (7.7%). The highest proportion of isolates was assigned to NWT against amoxicillin (80.6%), followed by oxytetracycline (77.7%) and erythromycin (48.5%). The time-scale data showed that recently obtained serotypes Ib/Ic and II isolates tended to be categorized as NWT populations resistant to more antibiotics, possibly due to microbial adaptation to antibiotic pressure. ARGs responsible for resistance to oxytetracycline and erythromycin were found only in NWT populations in serotype Ia [tet(S) and erm(B), respectively], and serotype II [tet(M) and mef(J)-msr(I), respectively]. We also found that the mef-msr gene pair in S. parauberis serotype II might be involved in low-level resistance to erythromycin. IMPORTANCE: This study presents serotype distribution and antimicrobial susceptibility data along with the antimicrobial resistance genes (ARGs) of Streptococcus parauberis, which is an important bacterial fish pathogen worldwide. In particular, almost all oxytetracycline and erythromycin non-wild-type (NWT) populations harbored tet(S) or tet(M), and erm(B) or mef(J)-msr(I), respectively. Interestingly, these ARGs were distributed in a highly serotype-dependent manner, resulting in a clear correlation between the antibiogram and serotype distribution. Moreover, recent isolates belonging to serotypes Ib/Ic and II tended to be more frequently categorized as NWT against antimicrobials, including amoxicillin and cefalexin compared to old isolates, while a dramatic decrease in erythromycin and clindamycin NWT frequencies was observed in recent serotype Ia isolates, which lacked erm(B). These variations might be attributed to shifts in the antibiotics employed in South Korean aquaculture over time. The overall findings would provide important background knowledge for understanding the epidemiology of S. parauberis infection in aquaculture.
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spelling pubmed-105812492023-10-18 Serotype distribution and antibiogram of Streptococcus parauberis isolated from fish in South Korea Lee, Yoonhang Kim, Nameun Roh, HeyongJin Ho, Diem Tho Park, Jiyeon Lee, Ju Yeop Kim, Yoon-Jae Kang, Hyo-Young Lee, Jungmin Song, Jun-Young Kim, Ahran Kim, Myoung Sug Cho, Miyoung Choi, Hye Sung Park, Chan-Il Kim, Do-Hyung Microbiol Spectr Research Article Streptococcus parauberis is the dominant etiological agent of streptococcosis, the most devastating bacterial disease in the olive flounder farming industry in South Korea. In this study, the distribution of serotypes, antimicrobial susceptibility, and presence of antimicrobial resistance genes (ARGs) in S. parauberis isolates obtained between 1999 and 2021 was thoroughly investigated to gain insight into the dynamics of their presence and the relationship between serotypes and antimicrobial resistance. Disk diffusion testing of 103 isolates against 10 antimicrobial agents was performed, and epidemiological cut-off values generated through normalized resistance interpretation analysis were used to classify wild-type (WT) and non-wild-type (NWT) populations. Principal component analysis and hierarchical clustering were implemented to achieve an understanding on the relationship between serotypes and antimicrobial resistance patterns. PCR-based serotyping showed that serotype Ia (67.1%) was the most prevalent in South Korea, followed by serotypes Ib/Ic (25.2%) and II (7.7%). The highest proportion of isolates was assigned to NWT against amoxicillin (80.6%), followed by oxytetracycline (77.7%) and erythromycin (48.5%). The time-scale data showed that recently obtained serotypes Ib/Ic and II isolates tended to be categorized as NWT populations resistant to more antibiotics, possibly due to microbial adaptation to antibiotic pressure. ARGs responsible for resistance to oxytetracycline and erythromycin were found only in NWT populations in serotype Ia [tet(S) and erm(B), respectively], and serotype II [tet(M) and mef(J)-msr(I), respectively]. We also found that the mef-msr gene pair in S. parauberis serotype II might be involved in low-level resistance to erythromycin. IMPORTANCE: This study presents serotype distribution and antimicrobial susceptibility data along with the antimicrobial resistance genes (ARGs) of Streptococcus parauberis, which is an important bacterial fish pathogen worldwide. In particular, almost all oxytetracycline and erythromycin non-wild-type (NWT) populations harbored tet(S) or tet(M), and erm(B) or mef(J)-msr(I), respectively. Interestingly, these ARGs were distributed in a highly serotype-dependent manner, resulting in a clear correlation between the antibiogram and serotype distribution. Moreover, recent isolates belonging to serotypes Ib/Ic and II tended to be more frequently categorized as NWT against antimicrobials, including amoxicillin and cefalexin compared to old isolates, while a dramatic decrease in erythromycin and clindamycin NWT frequencies was observed in recent serotype Ia isolates, which lacked erm(B). These variations might be attributed to shifts in the antibiotics employed in South Korean aquaculture over time. The overall findings would provide important background knowledge for understanding the epidemiology of S. parauberis infection in aquaculture. American Society for Microbiology 2023-08-09 /pmc/articles/PMC10581249/ /pubmed/37555676 http://dx.doi.org/10.1128/spectrum.04400-22 Text en Copyright © 2023 Lee et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Lee, Yoonhang
Kim, Nameun
Roh, HeyongJin
Ho, Diem Tho
Park, Jiyeon
Lee, Ju Yeop
Kim, Yoon-Jae
Kang, Hyo-Young
Lee, Jungmin
Song, Jun-Young
Kim, Ahran
Kim, Myoung Sug
Cho, Miyoung
Choi, Hye Sung
Park, Chan-Il
Kim, Do-Hyung
Serotype distribution and antibiogram of Streptococcus parauberis isolated from fish in South Korea
title Serotype distribution and antibiogram of Streptococcus parauberis isolated from fish in South Korea
title_full Serotype distribution and antibiogram of Streptococcus parauberis isolated from fish in South Korea
title_fullStr Serotype distribution and antibiogram of Streptococcus parauberis isolated from fish in South Korea
title_full_unstemmed Serotype distribution and antibiogram of Streptococcus parauberis isolated from fish in South Korea
title_short Serotype distribution and antibiogram of Streptococcus parauberis isolated from fish in South Korea
title_sort serotype distribution and antibiogram of streptococcus parauberis isolated from fish in south korea
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581249/
https://www.ncbi.nlm.nih.gov/pubmed/37555676
http://dx.doi.org/10.1128/spectrum.04400-22
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