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Preexisting helminth challenge exacerbates infection and reactivation of gammaherpesvirus in tissue resident macrophages

Even though gammaherpesvirus and parasitic infections are endemic in parts of the world, there is a lack of understanding about the outcome of coinfection. In humans, coinfections usually occur sequentially, with fluctuating order and timing in different hosts. However, experimental studies in mice...

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Autores principales: Zarek, Christina M., Dende, Chaitanya, Coronado, Jaime, Pendse, Mihir, Dryden, Phillip, Hooper, Lora V., Reese, Tiffany A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581490/
https://www.ncbi.nlm.nih.gov/pubmed/37847677
http://dx.doi.org/10.1371/journal.ppat.1011691
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author Zarek, Christina M.
Dende, Chaitanya
Coronado, Jaime
Pendse, Mihir
Dryden, Phillip
Hooper, Lora V.
Reese, Tiffany A.
author_facet Zarek, Christina M.
Dende, Chaitanya
Coronado, Jaime
Pendse, Mihir
Dryden, Phillip
Hooper, Lora V.
Reese, Tiffany A.
author_sort Zarek, Christina M.
collection PubMed
description Even though gammaherpesvirus and parasitic infections are endemic in parts of the world, there is a lack of understanding about the outcome of coinfection. In humans, coinfections usually occur sequentially, with fluctuating order and timing in different hosts. However, experimental studies in mice generally do not address the variables of order and timing of coinfections. We sought to examine the variable of coinfection order in a system of gammaherpesvirus-helminth coinfection. Our previous work demonstrated that infection with the intestinal parasite, Heligmosomoides polygyrus, induced transient reactivation from latency of murine gammaherpesvirus-68 (MHV68). In this report, we reverse the order of coinfection, infecting with H. polygyrus first, followed by MHV68, and examined the effects of preexisting parasite infection on MHV68 acute and latent infection. We found that preexisting parasite infection increased the propensity of MHV68 to reactivate from latency. However, when we examined the mechanism for reactivation, we found that preexisting parasite infection increased the ability of MHV68 to reactivate in a vitamin A dependent manner, a distinct mechanism to what we found previously with parasite-induced reactivation after latency establishment. We determined that H. polygyrus infection increased both acute and latent MHV68 infection in a population of tissue resident macrophages, called large peritoneal macrophages. We demonstrate that this population of macrophages and vitamin A are required for increased acute and latent infection during parasite coinfection.
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spelling pubmed-105814902023-10-18 Preexisting helminth challenge exacerbates infection and reactivation of gammaherpesvirus in tissue resident macrophages Zarek, Christina M. Dende, Chaitanya Coronado, Jaime Pendse, Mihir Dryden, Phillip Hooper, Lora V. Reese, Tiffany A. PLoS Pathog Research Article Even though gammaherpesvirus and parasitic infections are endemic in parts of the world, there is a lack of understanding about the outcome of coinfection. In humans, coinfections usually occur sequentially, with fluctuating order and timing in different hosts. However, experimental studies in mice generally do not address the variables of order and timing of coinfections. We sought to examine the variable of coinfection order in a system of gammaherpesvirus-helminth coinfection. Our previous work demonstrated that infection with the intestinal parasite, Heligmosomoides polygyrus, induced transient reactivation from latency of murine gammaherpesvirus-68 (MHV68). In this report, we reverse the order of coinfection, infecting with H. polygyrus first, followed by MHV68, and examined the effects of preexisting parasite infection on MHV68 acute and latent infection. We found that preexisting parasite infection increased the propensity of MHV68 to reactivate from latency. However, when we examined the mechanism for reactivation, we found that preexisting parasite infection increased the ability of MHV68 to reactivate in a vitamin A dependent manner, a distinct mechanism to what we found previously with parasite-induced reactivation after latency establishment. We determined that H. polygyrus infection increased both acute and latent MHV68 infection in a population of tissue resident macrophages, called large peritoneal macrophages. We demonstrate that this population of macrophages and vitamin A are required for increased acute and latent infection during parasite coinfection. Public Library of Science 2023-10-17 /pmc/articles/PMC10581490/ /pubmed/37847677 http://dx.doi.org/10.1371/journal.ppat.1011691 Text en © 2023 Zarek et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Zarek, Christina M.
Dende, Chaitanya
Coronado, Jaime
Pendse, Mihir
Dryden, Phillip
Hooper, Lora V.
Reese, Tiffany A.
Preexisting helminth challenge exacerbates infection and reactivation of gammaherpesvirus in tissue resident macrophages
title Preexisting helminth challenge exacerbates infection and reactivation of gammaherpesvirus in tissue resident macrophages
title_full Preexisting helminth challenge exacerbates infection and reactivation of gammaherpesvirus in tissue resident macrophages
title_fullStr Preexisting helminth challenge exacerbates infection and reactivation of gammaherpesvirus in tissue resident macrophages
title_full_unstemmed Preexisting helminth challenge exacerbates infection and reactivation of gammaherpesvirus in tissue resident macrophages
title_short Preexisting helminth challenge exacerbates infection and reactivation of gammaherpesvirus in tissue resident macrophages
title_sort preexisting helminth challenge exacerbates infection and reactivation of gammaherpesvirus in tissue resident macrophages
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581490/
https://www.ncbi.nlm.nih.gov/pubmed/37847677
http://dx.doi.org/10.1371/journal.ppat.1011691
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