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Immunohistochemical analysis of extracellular signal-regulated kinase expression in mature and immature bulls’ testes and epididymides
Extracellular signal-regulated kinase (ERK) has been implicated in mammalian testicular and epididymal development. This study aimed to investigate ERK expression in the immature and mature testes and epididymides of bulls. We evaluated ERK expression using immunoblot analysis and immunohistochemist...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Czech Academy of Agricultural Sciences
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581511/ https://www.ncbi.nlm.nih.gov/pubmed/37982001 http://dx.doi.org/10.17221/34/2023-VETMED |
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author | Jang, Sungwoong Yun, Changjin Kim, Bohye Kang, Sohi Lee, Jeongmin Jeong, Sohee Cho, Yongho Kim, Sung-Ho Lee, Chang-Min Moon, Changjong Kim, Joong-Sun |
author_facet | Jang, Sungwoong Yun, Changjin Kim, Bohye Kang, Sohi Lee, Jeongmin Jeong, Sohee Cho, Yongho Kim, Sung-Ho Lee, Chang-Min Moon, Changjong Kim, Joong-Sun |
author_sort | Jang, Sungwoong |
collection | PubMed |
description | Extracellular signal-regulated kinase (ERK) has been implicated in mammalian testicular and epididymal development. This study aimed to investigate ERK expression in the immature and mature testes and epididymides of bulls. We evaluated ERK expression using immunoblot analysis and immunohistochemistry. Immunoblot analysis revealed that immature bull testes and epididymides had higher phosphorylated ERK (pERK) expression than mature bull testes and epididymides. pERK immunoreactivity was higher in immature epididymides than in immature testes. pERK was localised mostly in spermatogonia, undifferentiated sustentacular (Sertoli) cells, and interstitial (Leydig) cells in immature testes, as well as in some spermatocytes and spermatids in mature testes. In immature epididymides, the body and tail had higher pERK expression than the head, whereas pERK was broadly distributed throughout the stereocilia, basal cells, and connective tissues. pERK distribution in the head of mature epididymides was similar to that in immature epididymides, whereas few connective tissue cells were expressed in the body and tail of mature epididymides. Collectively, these results suggest that ERK is expressed in the testis and epididymis of immature and mature bulls with varying intensities, and the role of ERK in male reproductive organs may include the specific function of its development. |
format | Online Article Text |
id | pubmed-10581511 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Czech Academy of Agricultural Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-105815112023-11-17 Immunohistochemical analysis of extracellular signal-regulated kinase expression in mature and immature bulls’ testes and epididymides Jang, Sungwoong Yun, Changjin Kim, Bohye Kang, Sohi Lee, Jeongmin Jeong, Sohee Cho, Yongho Kim, Sung-Ho Lee, Chang-Min Moon, Changjong Kim, Joong-Sun Vet Med (Praha) Original Paper Extracellular signal-regulated kinase (ERK) has been implicated in mammalian testicular and epididymal development. This study aimed to investigate ERK expression in the immature and mature testes and epididymides of bulls. We evaluated ERK expression using immunoblot analysis and immunohistochemistry. Immunoblot analysis revealed that immature bull testes and epididymides had higher phosphorylated ERK (pERK) expression than mature bull testes and epididymides. pERK immunoreactivity was higher in immature epididymides than in immature testes. pERK was localised mostly in spermatogonia, undifferentiated sustentacular (Sertoli) cells, and interstitial (Leydig) cells in immature testes, as well as in some spermatocytes and spermatids in mature testes. In immature epididymides, the body and tail had higher pERK expression than the head, whereas pERK was broadly distributed throughout the stereocilia, basal cells, and connective tissues. pERK distribution in the head of mature epididymides was similar to that in immature epididymides, whereas few connective tissue cells were expressed in the body and tail of mature epididymides. Collectively, these results suggest that ERK is expressed in the testis and epididymis of immature and mature bulls with varying intensities, and the role of ERK in male reproductive organs may include the specific function of its development. Czech Academy of Agricultural Sciences 2023-06-28 /pmc/articles/PMC10581511/ /pubmed/37982001 http://dx.doi.org/10.17221/34/2023-VETMED Text en Copyright: © 2023 Jang et al. https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (https://creativecommons.org/licenses/by/4.0/) (CC BY-NC 4.0). |
spellingShingle | Original Paper Jang, Sungwoong Yun, Changjin Kim, Bohye Kang, Sohi Lee, Jeongmin Jeong, Sohee Cho, Yongho Kim, Sung-Ho Lee, Chang-Min Moon, Changjong Kim, Joong-Sun Immunohistochemical analysis of extracellular signal-regulated kinase expression in mature and immature bulls’ testes and epididymides |
title | Immunohistochemical analysis of extracellular signal-regulated kinase expression in mature and immature bulls’ testes and epididymides |
title_full | Immunohistochemical analysis of extracellular signal-regulated kinase expression in mature and immature bulls’ testes and epididymides |
title_fullStr | Immunohistochemical analysis of extracellular signal-regulated kinase expression in mature and immature bulls’ testes and epididymides |
title_full_unstemmed | Immunohistochemical analysis of extracellular signal-regulated kinase expression in mature and immature bulls’ testes and epididymides |
title_short | Immunohistochemical analysis of extracellular signal-regulated kinase expression in mature and immature bulls’ testes and epididymides |
title_sort | immunohistochemical analysis of extracellular signal-regulated kinase expression in mature and immature bulls’ testes and epididymides |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581511/ https://www.ncbi.nlm.nih.gov/pubmed/37982001 http://dx.doi.org/10.17221/34/2023-VETMED |
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