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The effect of cold atmospheric plasma (NO) alone and in combination with NPH insulin on the full-thickness excisional wound healing in a diabetic rat model
This study was planned to investigate an alternative treatment modality in diabetic wound healing. In this experimental study, the efficacy of both cold atmospheric plasma/nitric oxide (NO) and NPH insulin ointment, recently known to have beneficial effects on wound healing, was investigated in diab...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Czech Academy of Agricultural Sciences
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581533/ https://www.ncbi.nlm.nih.gov/pubmed/37982089 http://dx.doi.org/10.17221/109/2022-VETMED |
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author | Curukoglu, Ali Gungor, Gul Ciray Akbas Akan, Gokce Kukner, Aysel Ogutcu, Gozde Kalayci, Melis Temizel, Meliha Ozgencil, Fatma Eser |
author_facet | Curukoglu, Ali Gungor, Gul Ciray Akbas Akan, Gokce Kukner, Aysel Ogutcu, Gozde Kalayci, Melis Temizel, Meliha Ozgencil, Fatma Eser |
author_sort | Curukoglu, Ali |
collection | PubMed |
description | This study was planned to investigate an alternative treatment modality in diabetic wound healing. In this experimental study, the efficacy of both cold atmospheric plasma/nitric oxide (NO) and NPH insulin ointment, recently known to have beneficial effects on wound healing, was investigated in diabetic wound healing. Twenty-four (24) diabetic rats were divided into four groups DC, DI, DNO and DINO (diabetic control, diabetic insulin, diabetic nitric oxide, diabetic insulin + nitric oxide groups). No treatment was applied to the DC group, NPH insulin was applied to the DI group, CAP/NO was applied to the DNO group, and CAP/NO + NPH insulin was applied to the DINO group once daily for 14 days. The wound area reduction and the wound contraction rate were calculated on the basis of the tissue sections taken, and histopathological and genetic analyses were carried out. Compared to the control group, exogenous NO gas was found to be a potent antibacterial agent in the diabetic wound healing, causing a reduction in the wound area (P = 0.034), an increased contraction rate (P = 0.021), epithelialisation (P = 0.02), collagen organisation (P = 0.006) and a reduction in the number of inflammatory cells (P = 0.002). A significant increase in the expression of IL-8 mRNA was observed (P = 0.026). It was concluded that NPH insulin alone contributes to wound healing, but it is not necessary to use it together with exogenous NO gas. |
format | Online Article Text |
id | pubmed-10581533 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Czech Academy of Agricultural Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-105815332023-11-17 The effect of cold atmospheric plasma (NO) alone and in combination with NPH insulin on the full-thickness excisional wound healing in a diabetic rat model Curukoglu, Ali Gungor, Gul Ciray Akbas Akan, Gokce Kukner, Aysel Ogutcu, Gozde Kalayci, Melis Temizel, Meliha Ozgencil, Fatma Eser Vet Med (Praha) Original Paper This study was planned to investigate an alternative treatment modality in diabetic wound healing. In this experimental study, the efficacy of both cold atmospheric plasma/nitric oxide (NO) and NPH insulin ointment, recently known to have beneficial effects on wound healing, was investigated in diabetic wound healing. Twenty-four (24) diabetic rats were divided into four groups DC, DI, DNO and DINO (diabetic control, diabetic insulin, diabetic nitric oxide, diabetic insulin + nitric oxide groups). No treatment was applied to the DC group, NPH insulin was applied to the DI group, CAP/NO was applied to the DNO group, and CAP/NO + NPH insulin was applied to the DINO group once daily for 14 days. The wound area reduction and the wound contraction rate were calculated on the basis of the tissue sections taken, and histopathological and genetic analyses were carried out. Compared to the control group, exogenous NO gas was found to be a potent antibacterial agent in the diabetic wound healing, causing a reduction in the wound area (P = 0.034), an increased contraction rate (P = 0.021), epithelialisation (P = 0.02), collagen organisation (P = 0.006) and a reduction in the number of inflammatory cells (P = 0.002). A significant increase in the expression of IL-8 mRNA was observed (P = 0.026). It was concluded that NPH insulin alone contributes to wound healing, but it is not necessary to use it together with exogenous NO gas. Czech Academy of Agricultural Sciences 2023-04-28 /pmc/articles/PMC10581533/ /pubmed/37982089 http://dx.doi.org/10.17221/109/2022-VETMED Text en Copyright: © 2023 Curukoglu et al. https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International (https://creativecommons.org/licenses/by/4.0/) (CC BY-NC 4.0). |
spellingShingle | Original Paper Curukoglu, Ali Gungor, Gul Ciray Akbas Akan, Gokce Kukner, Aysel Ogutcu, Gozde Kalayci, Melis Temizel, Meliha Ozgencil, Fatma Eser The effect of cold atmospheric plasma (NO) alone and in combination with NPH insulin on the full-thickness excisional wound healing in a diabetic rat model |
title | The effect of cold atmospheric plasma (NO) alone and in combination with NPH insulin on the full-thickness excisional wound healing in a diabetic rat model |
title_full | The effect of cold atmospheric plasma (NO) alone and in combination with NPH insulin on the full-thickness excisional wound healing in a diabetic rat model |
title_fullStr | The effect of cold atmospheric plasma (NO) alone and in combination with NPH insulin on the full-thickness excisional wound healing in a diabetic rat model |
title_full_unstemmed | The effect of cold atmospheric plasma (NO) alone and in combination with NPH insulin on the full-thickness excisional wound healing in a diabetic rat model |
title_short | The effect of cold atmospheric plasma (NO) alone and in combination with NPH insulin on the full-thickness excisional wound healing in a diabetic rat model |
title_sort | effect of cold atmospheric plasma (no) alone and in combination with nph insulin on the full-thickness excisional wound healing in a diabetic rat model |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581533/ https://www.ncbi.nlm.nih.gov/pubmed/37982089 http://dx.doi.org/10.17221/109/2022-VETMED |
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