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Scheduled dosage regimen by irreversible electroporation of loaded erythrocytes for cancer treatment
Precise control of cargo release is essential but still a great challenge for any drug delivery system. Irreversible electroporation (IRE), utilizing short high-voltage pulsed electric fields to destabilize the biological membrane, has been recently approved as a non-thermal technique for tumor abla...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AIP Publishing LLC
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581719/ https://www.ncbi.nlm.nih.gov/pubmed/37854061 http://dx.doi.org/10.1063/5.0174353 |
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author | Peng, Wencheng Yue, Yaqi Zhang, Yuting Li, Hao Zhang, Cao Wang, Peiyuan Cao, Yanbing Liu, Xiaolong Dong, Shoulong Wu, Ming Yao, Chenguo |
author_facet | Peng, Wencheng Yue, Yaqi Zhang, Yuting Li, Hao Zhang, Cao Wang, Peiyuan Cao, Yanbing Liu, Xiaolong Dong, Shoulong Wu, Ming Yao, Chenguo |
author_sort | Peng, Wencheng |
collection | PubMed |
description | Precise control of cargo release is essential but still a great challenge for any drug delivery system. Irreversible electroporation (IRE), utilizing short high-voltage pulsed electric fields to destabilize the biological membrane, has been recently approved as a non-thermal technique for tumor ablation without destroying the integrity of adjacent collagenous structures. Due to the electro-permeating membrane ability, IRE might also have great potential to realize the controlled drug release in response to various input IRE parameters, which were tested in a red blood cell (RBC) model in this work. According to the mathematical simulation model of a round biconcave disc-like cell based on RBC shape and dielectric characteristics, the permeability and the pore density of the RBC membrane were found to quantitatively depend on the pulse parameters. To further provide solid experimental evidence, indocyanine green (ICG) and doxorubicin (DOX) were both loaded inside RBCs (RBC@DOX&ICG) and the drug release rates were found to be tailorable by microsecond pulsed electric field (μsPEF). In addition, μsPEF could effectively modulate the tumor stroma to augment therapy efficacy by increasing micro-vessel density and permeability, softening extracellular matrix, and alleviating tumor hypoxia. Benefiting from these advantages, this IRE-responsive RBC@DOX&ICG achieved a remarkably synergistic anti-cancer effect by the combination of μsPEF and chemotherapy in the tumor-bearing mice model, with the survival time increasing above 90 days without tumor burden. Given that IRE is easily adaptable to different plasma membrane-based vehicles for delivering diverse drugs, this approach could offer a general applicability for cancer treatment. |
format | Online Article Text |
id | pubmed-10581719 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | AIP Publishing LLC |
record_format | MEDLINE/PubMed |
spelling | pubmed-105817192023-10-18 Scheduled dosage regimen by irreversible electroporation of loaded erythrocytes for cancer treatment Peng, Wencheng Yue, Yaqi Zhang, Yuting Li, Hao Zhang, Cao Wang, Peiyuan Cao, Yanbing Liu, Xiaolong Dong, Shoulong Wu, Ming Yao, Chenguo APL Bioeng Articles Precise control of cargo release is essential but still a great challenge for any drug delivery system. Irreversible electroporation (IRE), utilizing short high-voltage pulsed electric fields to destabilize the biological membrane, has been recently approved as a non-thermal technique for tumor ablation without destroying the integrity of adjacent collagenous structures. Due to the electro-permeating membrane ability, IRE might also have great potential to realize the controlled drug release in response to various input IRE parameters, which were tested in a red blood cell (RBC) model in this work. According to the mathematical simulation model of a round biconcave disc-like cell based on RBC shape and dielectric characteristics, the permeability and the pore density of the RBC membrane were found to quantitatively depend on the pulse parameters. To further provide solid experimental evidence, indocyanine green (ICG) and doxorubicin (DOX) were both loaded inside RBCs (RBC@DOX&ICG) and the drug release rates were found to be tailorable by microsecond pulsed electric field (μsPEF). In addition, μsPEF could effectively modulate the tumor stroma to augment therapy efficacy by increasing micro-vessel density and permeability, softening extracellular matrix, and alleviating tumor hypoxia. Benefiting from these advantages, this IRE-responsive RBC@DOX&ICG achieved a remarkably synergistic anti-cancer effect by the combination of μsPEF and chemotherapy in the tumor-bearing mice model, with the survival time increasing above 90 days without tumor burden. Given that IRE is easily adaptable to different plasma membrane-based vehicles for delivering diverse drugs, this approach could offer a general applicability for cancer treatment. AIP Publishing LLC 2023-10-16 /pmc/articles/PMC10581719/ /pubmed/37854061 http://dx.doi.org/10.1063/5.0174353 Text en © 2023 Author(s). https://creativecommons.org/licenses/by/4.0/All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ). |
spellingShingle | Articles Peng, Wencheng Yue, Yaqi Zhang, Yuting Li, Hao Zhang, Cao Wang, Peiyuan Cao, Yanbing Liu, Xiaolong Dong, Shoulong Wu, Ming Yao, Chenguo Scheduled dosage regimen by irreversible electroporation of loaded erythrocytes for cancer treatment |
title | Scheduled dosage regimen by irreversible electroporation of loaded erythrocytes for cancer treatment |
title_full | Scheduled dosage regimen by irreversible electroporation of loaded erythrocytes for cancer treatment |
title_fullStr | Scheduled dosage regimen by irreversible electroporation of loaded erythrocytes for cancer treatment |
title_full_unstemmed | Scheduled dosage regimen by irreversible electroporation of loaded erythrocytes for cancer treatment |
title_short | Scheduled dosage regimen by irreversible electroporation of loaded erythrocytes for cancer treatment |
title_sort | scheduled dosage regimen by irreversible electroporation of loaded erythrocytes for cancer treatment |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581719/ https://www.ncbi.nlm.nih.gov/pubmed/37854061 http://dx.doi.org/10.1063/5.0174353 |
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