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Scheduled dosage regimen by irreversible electroporation of loaded erythrocytes for cancer treatment

Precise control of cargo release is essential but still a great challenge for any drug delivery system. Irreversible electroporation (IRE), utilizing short high-voltage pulsed electric fields to destabilize the biological membrane, has been recently approved as a non-thermal technique for tumor abla...

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Autores principales: Peng, Wencheng, Yue, Yaqi, Zhang, Yuting, Li, Hao, Zhang, Cao, Wang, Peiyuan, Cao, Yanbing, Liu, Xiaolong, Dong, Shoulong, Wu, Ming, Yao, Chenguo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AIP Publishing LLC 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581719/
https://www.ncbi.nlm.nih.gov/pubmed/37854061
http://dx.doi.org/10.1063/5.0174353
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author Peng, Wencheng
Yue, Yaqi
Zhang, Yuting
Li, Hao
Zhang, Cao
Wang, Peiyuan
Cao, Yanbing
Liu, Xiaolong
Dong, Shoulong
Wu, Ming
Yao, Chenguo
author_facet Peng, Wencheng
Yue, Yaqi
Zhang, Yuting
Li, Hao
Zhang, Cao
Wang, Peiyuan
Cao, Yanbing
Liu, Xiaolong
Dong, Shoulong
Wu, Ming
Yao, Chenguo
author_sort Peng, Wencheng
collection PubMed
description Precise control of cargo release is essential but still a great challenge for any drug delivery system. Irreversible electroporation (IRE), utilizing short high-voltage pulsed electric fields to destabilize the biological membrane, has been recently approved as a non-thermal technique for tumor ablation without destroying the integrity of adjacent collagenous structures. Due to the electro-permeating membrane ability, IRE might also have great potential to realize the controlled drug release in response to various input IRE parameters, which were tested in a red blood cell (RBC) model in this work. According to the mathematical simulation model of a round biconcave disc-like cell based on RBC shape and dielectric characteristics, the permeability and the pore density of the RBC membrane were found to quantitatively depend on the pulse parameters. To further provide solid experimental evidence, indocyanine green (ICG) and doxorubicin (DOX) were both loaded inside RBCs (RBC@DOX&ICG) and the drug release rates were found to be tailorable by microsecond pulsed electric field (μsPEF). In addition, μsPEF could effectively modulate the tumor stroma to augment therapy efficacy by increasing micro-vessel density and permeability, softening extracellular matrix, and alleviating tumor hypoxia. Benefiting from these advantages, this IRE-responsive RBC@DOX&ICG achieved a remarkably synergistic anti-cancer effect by the combination of μsPEF and chemotherapy in the tumor-bearing mice model, with the survival time increasing above 90 days without tumor burden. Given that IRE is easily adaptable to different plasma membrane-based vehicles for delivering diverse drugs, this approach could offer a general applicability for cancer treatment.
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spelling pubmed-105817192023-10-18 Scheduled dosage regimen by irreversible electroporation of loaded erythrocytes for cancer treatment Peng, Wencheng Yue, Yaqi Zhang, Yuting Li, Hao Zhang, Cao Wang, Peiyuan Cao, Yanbing Liu, Xiaolong Dong, Shoulong Wu, Ming Yao, Chenguo APL Bioeng Articles Precise control of cargo release is essential but still a great challenge for any drug delivery system. Irreversible electroporation (IRE), utilizing short high-voltage pulsed electric fields to destabilize the biological membrane, has been recently approved as a non-thermal technique for tumor ablation without destroying the integrity of adjacent collagenous structures. Due to the electro-permeating membrane ability, IRE might also have great potential to realize the controlled drug release in response to various input IRE parameters, which were tested in a red blood cell (RBC) model in this work. According to the mathematical simulation model of a round biconcave disc-like cell based on RBC shape and dielectric characteristics, the permeability and the pore density of the RBC membrane were found to quantitatively depend on the pulse parameters. To further provide solid experimental evidence, indocyanine green (ICG) and doxorubicin (DOX) were both loaded inside RBCs (RBC@DOX&ICG) and the drug release rates were found to be tailorable by microsecond pulsed electric field (μsPEF). In addition, μsPEF could effectively modulate the tumor stroma to augment therapy efficacy by increasing micro-vessel density and permeability, softening extracellular matrix, and alleviating tumor hypoxia. Benefiting from these advantages, this IRE-responsive RBC@DOX&ICG achieved a remarkably synergistic anti-cancer effect by the combination of μsPEF and chemotherapy in the tumor-bearing mice model, with the survival time increasing above 90 days without tumor burden. Given that IRE is easily adaptable to different plasma membrane-based vehicles for delivering diverse drugs, this approach could offer a general applicability for cancer treatment. AIP Publishing LLC 2023-10-16 /pmc/articles/PMC10581719/ /pubmed/37854061 http://dx.doi.org/10.1063/5.0174353 Text en © 2023 Author(s). https://creativecommons.org/licenses/by/4.0/All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) ).
spellingShingle Articles
Peng, Wencheng
Yue, Yaqi
Zhang, Yuting
Li, Hao
Zhang, Cao
Wang, Peiyuan
Cao, Yanbing
Liu, Xiaolong
Dong, Shoulong
Wu, Ming
Yao, Chenguo
Scheduled dosage regimen by irreversible electroporation of loaded erythrocytes for cancer treatment
title Scheduled dosage regimen by irreversible electroporation of loaded erythrocytes for cancer treatment
title_full Scheduled dosage regimen by irreversible electroporation of loaded erythrocytes for cancer treatment
title_fullStr Scheduled dosage regimen by irreversible electroporation of loaded erythrocytes for cancer treatment
title_full_unstemmed Scheduled dosage regimen by irreversible electroporation of loaded erythrocytes for cancer treatment
title_short Scheduled dosage regimen by irreversible electroporation of loaded erythrocytes for cancer treatment
title_sort scheduled dosage regimen by irreversible electroporation of loaded erythrocytes for cancer treatment
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581719/
https://www.ncbi.nlm.nih.gov/pubmed/37854061
http://dx.doi.org/10.1063/5.0174353
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