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Temperature activated transient receptor potential ion channels from Antarctic fishes
Antarctic notothenioid fishes (cryonotothenioids) live in waters that range between −1.86°C and an extreme maximum +4°C. Evidence suggests these fish sense temperature peripherally, but the molecular mechanism of temperature sensation in unknown. Previous work identified transient receptor potential...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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The Royal Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581778/ https://www.ncbi.nlm.nih.gov/pubmed/37848053 http://dx.doi.org/10.1098/rsob.230215 |
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author | York, Julia M. |
author_facet | York, Julia M. |
author_sort | York, Julia M. |
collection | PubMed |
description | Antarctic notothenioid fishes (cryonotothenioids) live in waters that range between −1.86°C and an extreme maximum +4°C. Evidence suggests these fish sense temperature peripherally, but the molecular mechanism of temperature sensation in unknown. Previous work identified transient receptor potential (TRP) channels TRPA1b, TRPM4 and TRPV1a as the top candidates for temperature sensors. Here, cryonotothenioid TRPA1b and TRPV1a are characterized using Xenopus oocyte electrophysiology. TRPA1b and TRPV1a showed heat-evoked currents with Q10s of 11.1 ± 2.2 and 20.5 ± 2.4, respectively. Unexpectedly, heat activation occurred at a threshold of 22.9 ± 1.3°C for TRPA1b and 32.1 ± 0.6°C for TRPV1a. These fish have not experienced such temperatures for at least 15 Myr. Either (1) another molecular mechanism underlies temperature sensation, (2) these fishes do not sense temperatures below these thresholds despite having lethal limits as low as 5°C, or (3) native cellular conditions modify the TRP channels to function at relevant temperatures. The effects of osmolytes, pH, oxidation, phosphorylation, lipids and accessory proteins were tested. No conditions shifted the activity range of TRPV1a. Oxidation in combination with reduced cholesterol significantly dropped activation threshold of TRPA1b to 11.3 ± 2.3°C, it is hypothesized the effect may be due to lipid raft disruption. |
format | Online Article Text |
id | pubmed-10581778 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The Royal Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-105817782023-10-18 Temperature activated transient receptor potential ion channels from Antarctic fishes York, Julia M. Open Biol Research Antarctic notothenioid fishes (cryonotothenioids) live in waters that range between −1.86°C and an extreme maximum +4°C. Evidence suggests these fish sense temperature peripherally, but the molecular mechanism of temperature sensation in unknown. Previous work identified transient receptor potential (TRP) channels TRPA1b, TRPM4 and TRPV1a as the top candidates for temperature sensors. Here, cryonotothenioid TRPA1b and TRPV1a are characterized using Xenopus oocyte electrophysiology. TRPA1b and TRPV1a showed heat-evoked currents with Q10s of 11.1 ± 2.2 and 20.5 ± 2.4, respectively. Unexpectedly, heat activation occurred at a threshold of 22.9 ± 1.3°C for TRPA1b and 32.1 ± 0.6°C for TRPV1a. These fish have not experienced such temperatures for at least 15 Myr. Either (1) another molecular mechanism underlies temperature sensation, (2) these fishes do not sense temperatures below these thresholds despite having lethal limits as low as 5°C, or (3) native cellular conditions modify the TRP channels to function at relevant temperatures. The effects of osmolytes, pH, oxidation, phosphorylation, lipids and accessory proteins were tested. No conditions shifted the activity range of TRPV1a. Oxidation in combination with reduced cholesterol significantly dropped activation threshold of TRPA1b to 11.3 ± 2.3°C, it is hypothesized the effect may be due to lipid raft disruption. The Royal Society 2023-10-18 /pmc/articles/PMC10581778/ /pubmed/37848053 http://dx.doi.org/10.1098/rsob.230215 Text en © 2023 The Authors. https://creativecommons.org/licenses/by/4.0/Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, provided the original author and source are credited. |
spellingShingle | Research York, Julia M. Temperature activated transient receptor potential ion channels from Antarctic fishes |
title | Temperature activated transient receptor potential ion channels from Antarctic fishes |
title_full | Temperature activated transient receptor potential ion channels from Antarctic fishes |
title_fullStr | Temperature activated transient receptor potential ion channels from Antarctic fishes |
title_full_unstemmed | Temperature activated transient receptor potential ion channels from Antarctic fishes |
title_short | Temperature activated transient receptor potential ion channels from Antarctic fishes |
title_sort | temperature activated transient receptor potential ion channels from antarctic fishes |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581778/ https://www.ncbi.nlm.nih.gov/pubmed/37848053 http://dx.doi.org/10.1098/rsob.230215 |
work_keys_str_mv | AT yorkjuliam temperatureactivatedtransientreceptorpotentialionchannelsfromantarcticfishes |