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Optimization Conditions of Ultrasound-Assisted Extraction for Phenolic Compounds and Antioxidant Activity from Rubus alceifolius Poir Leaves

Rubus alceifolius Poir (R.A. Poir) leaves are rich in phenolic compounds, offering many health benefits due to their incredible antioxidant potential. In this study, conditions for the ultrasound-assisted extraction (UAE) of phenolic compounds and antioxidant activity from R.A. Poir leaves were opti...

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Autores principales: Tran, Chi Hai, Nghiem, Minh Tri, Dinh, Anh Minh Trinh, Dang, Thi Thuy Nga, Van Do, Thi Thuy, Chu, Thi Nga, Mai, Tien Hung, Phan, Van Man
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581860/
https://www.ncbi.nlm.nih.gov/pubmed/37854461
http://dx.doi.org/10.1155/2023/7576179
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author Tran, Chi Hai
Nghiem, Minh Tri
Dinh, Anh Minh Trinh
Dang, Thi Thuy Nga
Van Do, Thi Thuy
Chu, Thi Nga
Mai, Tien Hung
Phan, Van Man
author_facet Tran, Chi Hai
Nghiem, Minh Tri
Dinh, Anh Minh Trinh
Dang, Thi Thuy Nga
Van Do, Thi Thuy
Chu, Thi Nga
Mai, Tien Hung
Phan, Van Man
author_sort Tran, Chi Hai
collection PubMed
description Rubus alceifolius Poir (R.A. Poir) leaves are rich in phenolic compounds, offering many health benefits due to their incredible antioxidant potential. In this study, conditions for the ultrasound-assisted extraction (UAE) of phenolic compounds and antioxidant activity from R.A. Poir leaves were optimized using response surface methodology (RSM). This methodology assessed the effects of ultrasound power (X(1): 100-500 W), extraction temperature (X(2): 30-60°C), and extraction time (X(3): 5-55 min). The optimized UAE conditions were then compared with conventional extraction methods (Soxhlet extraction: SE and maceration extraction: ME) for extracting total phenolics. A phenolic profile using GC-MS and antioxidant activity (ABTS) was also compared. According to the RSM, the best conditions for UAE to extract the highest total polyphenol content and ABTS radical scavenging activity were 320 W ultrasound power, 40°C extraction temperature, and 35.5 min sonication duration. Under these optimal conditions, the TPC and antioxidant activity reached 16.68 mg GAE/g dm and 21.9 mg TE/g, respectively, closely aligning with the predicted values. The UAE extraction technique proved to be more efficient in extracting phenolics and antioxidant capacity (ABTS (2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)) radical scavenging activity, and enzyme inhibition) compared to the conventional extraction methods (SE and ME). A GC-MS analysis identified 12 components, including 5 phenolics and 3 flavonoids, which likely contribute to the antioxidant activity. Consequently, the UAE method improved extraction efficiency within a shorter time frame, suggesting that UAE is a promising, efficient, and ecofriendly technology for extracting bioactive compounds from R.A. Poir leaves.
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spelling pubmed-105818602023-10-18 Optimization Conditions of Ultrasound-Assisted Extraction for Phenolic Compounds and Antioxidant Activity from Rubus alceifolius Poir Leaves Tran, Chi Hai Nghiem, Minh Tri Dinh, Anh Minh Trinh Dang, Thi Thuy Nga Van Do, Thi Thuy Chu, Thi Nga Mai, Tien Hung Phan, Van Man Int J Food Sci Research Article Rubus alceifolius Poir (R.A. Poir) leaves are rich in phenolic compounds, offering many health benefits due to their incredible antioxidant potential. In this study, conditions for the ultrasound-assisted extraction (UAE) of phenolic compounds and antioxidant activity from R.A. Poir leaves were optimized using response surface methodology (RSM). This methodology assessed the effects of ultrasound power (X(1): 100-500 W), extraction temperature (X(2): 30-60°C), and extraction time (X(3): 5-55 min). The optimized UAE conditions were then compared with conventional extraction methods (Soxhlet extraction: SE and maceration extraction: ME) for extracting total phenolics. A phenolic profile using GC-MS and antioxidant activity (ABTS) was also compared. According to the RSM, the best conditions for UAE to extract the highest total polyphenol content and ABTS radical scavenging activity were 320 W ultrasound power, 40°C extraction temperature, and 35.5 min sonication duration. Under these optimal conditions, the TPC and antioxidant activity reached 16.68 mg GAE/g dm and 21.9 mg TE/g, respectively, closely aligning with the predicted values. The UAE extraction technique proved to be more efficient in extracting phenolics and antioxidant capacity (ABTS (2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid)) radical scavenging activity, and enzyme inhibition) compared to the conventional extraction methods (SE and ME). A GC-MS analysis identified 12 components, including 5 phenolics and 3 flavonoids, which likely contribute to the antioxidant activity. Consequently, the UAE method improved extraction efficiency within a shorter time frame, suggesting that UAE is a promising, efficient, and ecofriendly technology for extracting bioactive compounds from R.A. Poir leaves. Hindawi 2023-10-10 /pmc/articles/PMC10581860/ /pubmed/37854461 http://dx.doi.org/10.1155/2023/7576179 Text en Copyright © 2023 Chi Hai Tran et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tran, Chi Hai
Nghiem, Minh Tri
Dinh, Anh Minh Trinh
Dang, Thi Thuy Nga
Van Do, Thi Thuy
Chu, Thi Nga
Mai, Tien Hung
Phan, Van Man
Optimization Conditions of Ultrasound-Assisted Extraction for Phenolic Compounds and Antioxidant Activity from Rubus alceifolius Poir Leaves
title Optimization Conditions of Ultrasound-Assisted Extraction for Phenolic Compounds and Antioxidant Activity from Rubus alceifolius Poir Leaves
title_full Optimization Conditions of Ultrasound-Assisted Extraction for Phenolic Compounds and Antioxidant Activity from Rubus alceifolius Poir Leaves
title_fullStr Optimization Conditions of Ultrasound-Assisted Extraction for Phenolic Compounds and Antioxidant Activity from Rubus alceifolius Poir Leaves
title_full_unstemmed Optimization Conditions of Ultrasound-Assisted Extraction for Phenolic Compounds and Antioxidant Activity from Rubus alceifolius Poir Leaves
title_short Optimization Conditions of Ultrasound-Assisted Extraction for Phenolic Compounds and Antioxidant Activity from Rubus alceifolius Poir Leaves
title_sort optimization conditions of ultrasound-assisted extraction for phenolic compounds and antioxidant activity from rubus alceifolius poir leaves
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10581860/
https://www.ncbi.nlm.nih.gov/pubmed/37854461
http://dx.doi.org/10.1155/2023/7576179
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