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Net-spinning caddisflies create denitrifier-enriched niches in the stream microbiome
Larval net-spinning caddisflies (Hydropsychidae) function as ecosystem engineers in streams where they construct protective retreats composed of organic and inorganic material affixed with silk filtration nets that alter streambed hydrology. We hypothesized that hydropsychid bio-structures (retreats...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10582121/ https://www.ncbi.nlm.nih.gov/pubmed/37848489 http://dx.doi.org/10.1038/s43705-023-00315-8 |
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author | Bertagnolli, Anthony D. Maritan, Andrew J. Tumolo, Benjamin B. Fritz, Samuel F. Oakland, Hayley C. Mohr, Elizabeth J. Poole, Geoffrey C. Albertson, Lindsey K. Stewart, Frank J. |
author_facet | Bertagnolli, Anthony D. Maritan, Andrew J. Tumolo, Benjamin B. Fritz, Samuel F. Oakland, Hayley C. Mohr, Elizabeth J. Poole, Geoffrey C. Albertson, Lindsey K. Stewart, Frank J. |
author_sort | Bertagnolli, Anthony D. |
collection | PubMed |
description | Larval net-spinning caddisflies (Hydropsychidae) function as ecosystem engineers in streams where they construct protective retreats composed of organic and inorganic material affixed with silk filtration nets that alter streambed hydrology. We hypothesized that hydropsychid bio-structures (retreats, nets) are microhabitats for microbes with oxygen-sensitive metabolisms, and therefore increase the metabolic heterogeneity of streambed microbial assemblages. Metagenomic and 16 S rRNA gene amplicon analysis of samples from a montane stream (Cherry Creek, Montana, USA) revealed that microbiomes of caddisfly bio-structures are taxonomically and functionally distinct from those of the immediately adjacent rock biofilm (~2 cm distant) and enriched in microbial taxa with established roles in denitrification, nitrification, and methane production. Genes for denitrification, high oxygen affinity terminal oxidases, hydrogenases, oxidative dissimilatory sulfite reductases, and complete ammonia oxidation are significantly enriched in caddisfly bio-structures. The results suggest a novel ecosystem engineering effect of caddisflies through the creation of low-oxygen, denitrifier-enriched niches in the stream microbiome. Facilitation of metabolic diversity in streambeds may be a largely unrecognized mechanism by which caddisflies alter whole-stream biogeochemistry. |
format | Online Article Text |
id | pubmed-10582121 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-105821212023-10-19 Net-spinning caddisflies create denitrifier-enriched niches in the stream microbiome Bertagnolli, Anthony D. Maritan, Andrew J. Tumolo, Benjamin B. Fritz, Samuel F. Oakland, Hayley C. Mohr, Elizabeth J. Poole, Geoffrey C. Albertson, Lindsey K. Stewart, Frank J. ISME Commun Brief Communication Larval net-spinning caddisflies (Hydropsychidae) function as ecosystem engineers in streams where they construct protective retreats composed of organic and inorganic material affixed with silk filtration nets that alter streambed hydrology. We hypothesized that hydropsychid bio-structures (retreats, nets) are microhabitats for microbes with oxygen-sensitive metabolisms, and therefore increase the metabolic heterogeneity of streambed microbial assemblages. Metagenomic and 16 S rRNA gene amplicon analysis of samples from a montane stream (Cherry Creek, Montana, USA) revealed that microbiomes of caddisfly bio-structures are taxonomically and functionally distinct from those of the immediately adjacent rock biofilm (~2 cm distant) and enriched in microbial taxa with established roles in denitrification, nitrification, and methane production. Genes for denitrification, high oxygen affinity terminal oxidases, hydrogenases, oxidative dissimilatory sulfite reductases, and complete ammonia oxidation are significantly enriched in caddisfly bio-structures. The results suggest a novel ecosystem engineering effect of caddisflies through the creation of low-oxygen, denitrifier-enriched niches in the stream microbiome. Facilitation of metabolic diversity in streambeds may be a largely unrecognized mechanism by which caddisflies alter whole-stream biogeochemistry. Nature Publishing Group UK 2023-10-17 /pmc/articles/PMC10582121/ /pubmed/37848489 http://dx.doi.org/10.1038/s43705-023-00315-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Brief Communication Bertagnolli, Anthony D. Maritan, Andrew J. Tumolo, Benjamin B. Fritz, Samuel F. Oakland, Hayley C. Mohr, Elizabeth J. Poole, Geoffrey C. Albertson, Lindsey K. Stewart, Frank J. Net-spinning caddisflies create denitrifier-enriched niches in the stream microbiome |
title | Net-spinning caddisflies create denitrifier-enriched niches in the stream microbiome |
title_full | Net-spinning caddisflies create denitrifier-enriched niches in the stream microbiome |
title_fullStr | Net-spinning caddisflies create denitrifier-enriched niches in the stream microbiome |
title_full_unstemmed | Net-spinning caddisflies create denitrifier-enriched niches in the stream microbiome |
title_short | Net-spinning caddisflies create denitrifier-enriched niches in the stream microbiome |
title_sort | net-spinning caddisflies create denitrifier-enriched niches in the stream microbiome |
topic | Brief Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10582121/ https://www.ncbi.nlm.nih.gov/pubmed/37848489 http://dx.doi.org/10.1038/s43705-023-00315-8 |
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