Cargando…

Experimental study on changes in metabolic mechanism of papillary thyroid carcinoma complicated with Hashimoto’s thyroiditis

BACKGROUND: Whether the mechanism of thyroid papillary carcinoma (PTC) is the same in patients with a Hashimoto's thyroiditis (HT) background as compared with patients with a normal background remains a highly debated and controversial issue. In this study, we aimed to analyze the differences a...

Descripción completa

Detalles Bibliográficos
Autores principales: Sun, Danyang, Zhang, Yujie, Wang, Dan, Zhao, Xue, Han, Rui, Li, Ning, Li, Xue, Li, Tingwei, Wang, Peng, Jia, Qiang, Tan, Jian, Zheng, Wei, Song, Lili, Meng, Zhaowei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10582305/
https://www.ncbi.nlm.nih.gov/pubmed/37860538
http://dx.doi.org/10.1016/j.heliyon.2023.e20661
_version_ 1785122300311371776
author Sun, Danyang
Zhang, Yujie
Wang, Dan
Zhao, Xue
Han, Rui
Li, Ning
Li, Xue
Li, Tingwei
Wang, Peng
Jia, Qiang
Tan, Jian
Zheng, Wei
Song, Lili
Meng, Zhaowei
author_facet Sun, Danyang
Zhang, Yujie
Wang, Dan
Zhao, Xue
Han, Rui
Li, Ning
Li, Xue
Li, Tingwei
Wang, Peng
Jia, Qiang
Tan, Jian
Zheng, Wei
Song, Lili
Meng, Zhaowei
author_sort Sun, Danyang
collection PubMed
description BACKGROUND: Whether the mechanism of thyroid papillary carcinoma (PTC) is the same in patients with a Hashimoto's thyroiditis (HT) background as compared with patients with a normal background remains a highly debated and controversial issue. In this study, we aimed to analyze the differences and similarities of the metabolic mechanism of PTC in normal and HT background, and to explore the relationship between HT and PTC. METHODS: The ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-Q-TOF/MS) technology was used to analyze 61 PTC patient tissues (31 HT background and 30 normal tissue (NC) background). Potential biomarkers were screened from principal component analysis (PCA) to orthogonal partial least square (OPLS) discriminant analysis. HMDB was searched to identify potential differential metabolites and final metabolic pathway analysis was performed by MetaboAnalyst 5.0. We analyzed the differential metabolites diagnostic accuracy through receiver operating characteristic (ROC) curves analysis. RESULTS: Seven different metabolites were screened from HT group and NC group, including arginine, glutamic acid, cysteine, citric acid, malic acid, uracil and taurine. Logistic regression model combined with ROC analysis of these 7 biomarkers had good discriminability for PTC (area under operating characteristic curve of HT group and NC group were 0.867 and 0.973, respectively). The HT group had specific metabolic pathways, including aminoacyl-tRNA biosynthesis, glycine, serine and threonine metabolism. CONCLUSIONS: The metabolic profiles of the NC and HT groups had important similarities and differences in PTC. The correlation of PTC with HT may be related to aminoacyl-tRNA biosynthesis, serine and threonine metabolism.
format Online
Article
Text
id pubmed-10582305
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-105823052023-10-19 Experimental study on changes in metabolic mechanism of papillary thyroid carcinoma complicated with Hashimoto’s thyroiditis Sun, Danyang Zhang, Yujie Wang, Dan Zhao, Xue Han, Rui Li, Ning Li, Xue Li, Tingwei Wang, Peng Jia, Qiang Tan, Jian Zheng, Wei Song, Lili Meng, Zhaowei Heliyon Research Article BACKGROUND: Whether the mechanism of thyroid papillary carcinoma (PTC) is the same in patients with a Hashimoto's thyroiditis (HT) background as compared with patients with a normal background remains a highly debated and controversial issue. In this study, we aimed to analyze the differences and similarities of the metabolic mechanism of PTC in normal and HT background, and to explore the relationship between HT and PTC. METHODS: The ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-Q-TOF/MS) technology was used to analyze 61 PTC patient tissues (31 HT background and 30 normal tissue (NC) background). Potential biomarkers were screened from principal component analysis (PCA) to orthogonal partial least square (OPLS) discriminant analysis. HMDB was searched to identify potential differential metabolites and final metabolic pathway analysis was performed by MetaboAnalyst 5.0. We analyzed the differential metabolites diagnostic accuracy through receiver operating characteristic (ROC) curves analysis. RESULTS: Seven different metabolites were screened from HT group and NC group, including arginine, glutamic acid, cysteine, citric acid, malic acid, uracil and taurine. Logistic regression model combined with ROC analysis of these 7 biomarkers had good discriminability for PTC (area under operating characteristic curve of HT group and NC group were 0.867 and 0.973, respectively). The HT group had specific metabolic pathways, including aminoacyl-tRNA biosynthesis, glycine, serine and threonine metabolism. CONCLUSIONS: The metabolic profiles of the NC and HT groups had important similarities and differences in PTC. The correlation of PTC with HT may be related to aminoacyl-tRNA biosynthesis, serine and threonine metabolism. Elsevier 2023-10-05 /pmc/articles/PMC10582305/ /pubmed/37860538 http://dx.doi.org/10.1016/j.heliyon.2023.e20661 Text en © 2023 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Research Article
Sun, Danyang
Zhang, Yujie
Wang, Dan
Zhao, Xue
Han, Rui
Li, Ning
Li, Xue
Li, Tingwei
Wang, Peng
Jia, Qiang
Tan, Jian
Zheng, Wei
Song, Lili
Meng, Zhaowei
Experimental study on changes in metabolic mechanism of papillary thyroid carcinoma complicated with Hashimoto’s thyroiditis
title Experimental study on changes in metabolic mechanism of papillary thyroid carcinoma complicated with Hashimoto’s thyroiditis
title_full Experimental study on changes in metabolic mechanism of papillary thyroid carcinoma complicated with Hashimoto’s thyroiditis
title_fullStr Experimental study on changes in metabolic mechanism of papillary thyroid carcinoma complicated with Hashimoto’s thyroiditis
title_full_unstemmed Experimental study on changes in metabolic mechanism of papillary thyroid carcinoma complicated with Hashimoto’s thyroiditis
title_short Experimental study on changes in metabolic mechanism of papillary thyroid carcinoma complicated with Hashimoto’s thyroiditis
title_sort experimental study on changes in metabolic mechanism of papillary thyroid carcinoma complicated with hashimoto’s thyroiditis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10582305/
https://www.ncbi.nlm.nih.gov/pubmed/37860538
http://dx.doi.org/10.1016/j.heliyon.2023.e20661
work_keys_str_mv AT sundanyang experimentalstudyonchangesinmetabolicmechanismofpapillarythyroidcarcinomacomplicatedwithhashimotosthyroiditis
AT zhangyujie experimentalstudyonchangesinmetabolicmechanismofpapillarythyroidcarcinomacomplicatedwithhashimotosthyroiditis
AT wangdan experimentalstudyonchangesinmetabolicmechanismofpapillarythyroidcarcinomacomplicatedwithhashimotosthyroiditis
AT zhaoxue experimentalstudyonchangesinmetabolicmechanismofpapillarythyroidcarcinomacomplicatedwithhashimotosthyroiditis
AT hanrui experimentalstudyonchangesinmetabolicmechanismofpapillarythyroidcarcinomacomplicatedwithhashimotosthyroiditis
AT lining experimentalstudyonchangesinmetabolicmechanismofpapillarythyroidcarcinomacomplicatedwithhashimotosthyroiditis
AT lixue experimentalstudyonchangesinmetabolicmechanismofpapillarythyroidcarcinomacomplicatedwithhashimotosthyroiditis
AT litingwei experimentalstudyonchangesinmetabolicmechanismofpapillarythyroidcarcinomacomplicatedwithhashimotosthyroiditis
AT wangpeng experimentalstudyonchangesinmetabolicmechanismofpapillarythyroidcarcinomacomplicatedwithhashimotosthyroiditis
AT jiaqiang experimentalstudyonchangesinmetabolicmechanismofpapillarythyroidcarcinomacomplicatedwithhashimotosthyroiditis
AT tanjian experimentalstudyonchangesinmetabolicmechanismofpapillarythyroidcarcinomacomplicatedwithhashimotosthyroiditis
AT zhengwei experimentalstudyonchangesinmetabolicmechanismofpapillarythyroidcarcinomacomplicatedwithhashimotosthyroiditis
AT songlili experimentalstudyonchangesinmetabolicmechanismofpapillarythyroidcarcinomacomplicatedwithhashimotosthyroiditis
AT mengzhaowei experimentalstudyonchangesinmetabolicmechanismofpapillarythyroidcarcinomacomplicatedwithhashimotosthyroiditis