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Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD

In recent years, meat adulteration safety incidents have occurred frequently, triggering widespread attention and discussion. Although there are a variety of meat quality identification methods, conventional assays require high standards for personnel and experimental conditions and are not suitable...

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Autores principales: Liu, Yaqun, Lin, Liyun, Wei, Huagui, Luo, Qiulan, Yang, Peikui, Liu, Mouquan, Wang, Zhonghe, Zou, Xianghui, Zhu, Hui, Zha, Guangcai, Sun, Junjun, Zheng, Yuzhong, Lin, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10582345/
https://www.ncbi.nlm.nih.gov/pubmed/37860145
http://dx.doi.org/10.1016/j.crfs.2023.100609
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author Liu, Yaqun
Lin, Liyun
Wei, Huagui
Luo, Qiulan
Yang, Peikui
Liu, Mouquan
Wang, Zhonghe
Zou, Xianghui
Zhu, Hui
Zha, Guangcai
Sun, Junjun
Zheng, Yuzhong
Lin, Min
author_facet Liu, Yaqun
Lin, Liyun
Wei, Huagui
Luo, Qiulan
Yang, Peikui
Liu, Mouquan
Wang, Zhonghe
Zou, Xianghui
Zhu, Hui
Zha, Guangcai
Sun, Junjun
Zheng, Yuzhong
Lin, Min
author_sort Liu, Yaqun
collection PubMed
description In recent years, meat adulteration safety incidents have occurred frequently, triggering widespread attention and discussion. Although there are a variety of meat quality identification methods, conventional assays require high standards for personnel and experimental conditions and are not suitable for on-site testing. Therefore, there is an urgent need for a rapid, sensitive, high specificity and high sensitivity on-site meat detection method. This study is the first to apply RPA combined with CRISPR/Cas12a technology to the field of multiple meat identification. The system developed by parameter optimization can achieve specific detection of chicken, duck, beef, pork and lamb with a minimum target sequence copy number as low as 1 × 10(0) copies/μL for 60 min at a constant temperature. LFD test results can be directly observed with the naked eye, with the characteristics of fast, portable and simple operation, which is extremely in line with current needs. In conclusion, the meat identification RPA-CRISPR/Cas12a-LFD system established in this study has shown promising applications in the field of meat detection, with a profound impact on meat quality, and provides a model for other food safety control programs.
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spelling pubmed-105823452023-10-19 Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD Liu, Yaqun Lin, Liyun Wei, Huagui Luo, Qiulan Yang, Peikui Liu, Mouquan Wang, Zhonghe Zou, Xianghui Zhu, Hui Zha, Guangcai Sun, Junjun Zheng, Yuzhong Lin, Min Curr Res Food Sci Research Article In recent years, meat adulteration safety incidents have occurred frequently, triggering widespread attention and discussion. Although there are a variety of meat quality identification methods, conventional assays require high standards for personnel and experimental conditions and are not suitable for on-site testing. Therefore, there is an urgent need for a rapid, sensitive, high specificity and high sensitivity on-site meat detection method. This study is the first to apply RPA combined with CRISPR/Cas12a technology to the field of multiple meat identification. The system developed by parameter optimization can achieve specific detection of chicken, duck, beef, pork and lamb with a minimum target sequence copy number as low as 1 × 10(0) copies/μL for 60 min at a constant temperature. LFD test results can be directly observed with the naked eye, with the characteristics of fast, portable and simple operation, which is extremely in line with current needs. In conclusion, the meat identification RPA-CRISPR/Cas12a-LFD system established in this study has shown promising applications in the field of meat detection, with a profound impact on meat quality, and provides a model for other food safety control programs. Elsevier 2023-10-05 /pmc/articles/PMC10582345/ /pubmed/37860145 http://dx.doi.org/10.1016/j.crfs.2023.100609 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Liu, Yaqun
Lin, Liyun
Wei, Huagui
Luo, Qiulan
Yang, Peikui
Liu, Mouquan
Wang, Zhonghe
Zou, Xianghui
Zhu, Hui
Zha, Guangcai
Sun, Junjun
Zheng, Yuzhong
Lin, Min
Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD
title Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD
title_full Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD
title_fullStr Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD
title_full_unstemmed Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD
title_short Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD
title_sort design and development of a rapid meat detection system based on rpa-crispr/cas12a-lfd
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10582345/
https://www.ncbi.nlm.nih.gov/pubmed/37860145
http://dx.doi.org/10.1016/j.crfs.2023.100609
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