Cargando…
Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD
In recent years, meat adulteration safety incidents have occurred frequently, triggering widespread attention and discussion. Although there are a variety of meat quality identification methods, conventional assays require high standards for personnel and experimental conditions and are not suitable...
Autores principales: | , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10582345/ https://www.ncbi.nlm.nih.gov/pubmed/37860145 http://dx.doi.org/10.1016/j.crfs.2023.100609 |
_version_ | 1785122309219024896 |
---|---|
author | Liu, Yaqun Lin, Liyun Wei, Huagui Luo, Qiulan Yang, Peikui Liu, Mouquan Wang, Zhonghe Zou, Xianghui Zhu, Hui Zha, Guangcai Sun, Junjun Zheng, Yuzhong Lin, Min |
author_facet | Liu, Yaqun Lin, Liyun Wei, Huagui Luo, Qiulan Yang, Peikui Liu, Mouquan Wang, Zhonghe Zou, Xianghui Zhu, Hui Zha, Guangcai Sun, Junjun Zheng, Yuzhong Lin, Min |
author_sort | Liu, Yaqun |
collection | PubMed |
description | In recent years, meat adulteration safety incidents have occurred frequently, triggering widespread attention and discussion. Although there are a variety of meat quality identification methods, conventional assays require high standards for personnel and experimental conditions and are not suitable for on-site testing. Therefore, there is an urgent need for a rapid, sensitive, high specificity and high sensitivity on-site meat detection method. This study is the first to apply RPA combined with CRISPR/Cas12a technology to the field of multiple meat identification. The system developed by parameter optimization can achieve specific detection of chicken, duck, beef, pork and lamb with a minimum target sequence copy number as low as 1 × 10(0) copies/μL for 60 min at a constant temperature. LFD test results can be directly observed with the naked eye, with the characteristics of fast, portable and simple operation, which is extremely in line with current needs. In conclusion, the meat identification RPA-CRISPR/Cas12a-LFD system established in this study has shown promising applications in the field of meat detection, with a profound impact on meat quality, and provides a model for other food safety control programs. |
format | Online Article Text |
id | pubmed-10582345 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-105823452023-10-19 Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD Liu, Yaqun Lin, Liyun Wei, Huagui Luo, Qiulan Yang, Peikui Liu, Mouquan Wang, Zhonghe Zou, Xianghui Zhu, Hui Zha, Guangcai Sun, Junjun Zheng, Yuzhong Lin, Min Curr Res Food Sci Research Article In recent years, meat adulteration safety incidents have occurred frequently, triggering widespread attention and discussion. Although there are a variety of meat quality identification methods, conventional assays require high standards for personnel and experimental conditions and are not suitable for on-site testing. Therefore, there is an urgent need for a rapid, sensitive, high specificity and high sensitivity on-site meat detection method. This study is the first to apply RPA combined with CRISPR/Cas12a technology to the field of multiple meat identification. The system developed by parameter optimization can achieve specific detection of chicken, duck, beef, pork and lamb with a minimum target sequence copy number as low as 1 × 10(0) copies/μL for 60 min at a constant temperature. LFD test results can be directly observed with the naked eye, with the characteristics of fast, portable and simple operation, which is extremely in line with current needs. In conclusion, the meat identification RPA-CRISPR/Cas12a-LFD system established in this study has shown promising applications in the field of meat detection, with a profound impact on meat quality, and provides a model for other food safety control programs. Elsevier 2023-10-05 /pmc/articles/PMC10582345/ /pubmed/37860145 http://dx.doi.org/10.1016/j.crfs.2023.100609 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Research Article Liu, Yaqun Lin, Liyun Wei, Huagui Luo, Qiulan Yang, Peikui Liu, Mouquan Wang, Zhonghe Zou, Xianghui Zhu, Hui Zha, Guangcai Sun, Junjun Zheng, Yuzhong Lin, Min Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD |
title | Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD |
title_full | Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD |
title_fullStr | Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD |
title_full_unstemmed | Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD |
title_short | Design and development of a rapid meat detection system based on RPA-CRISPR/Cas12a-LFD |
title_sort | design and development of a rapid meat detection system based on rpa-crispr/cas12a-lfd |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10582345/ https://www.ncbi.nlm.nih.gov/pubmed/37860145 http://dx.doi.org/10.1016/j.crfs.2023.100609 |
work_keys_str_mv | AT liuyaqun designanddevelopmentofarapidmeatdetectionsystembasedonrpacrisprcas12alfd AT linliyun designanddevelopmentofarapidmeatdetectionsystembasedonrpacrisprcas12alfd AT weihuagui designanddevelopmentofarapidmeatdetectionsystembasedonrpacrisprcas12alfd AT luoqiulan designanddevelopmentofarapidmeatdetectionsystembasedonrpacrisprcas12alfd AT yangpeikui designanddevelopmentofarapidmeatdetectionsystembasedonrpacrisprcas12alfd AT liumouquan designanddevelopmentofarapidmeatdetectionsystembasedonrpacrisprcas12alfd AT wangzhonghe designanddevelopmentofarapidmeatdetectionsystembasedonrpacrisprcas12alfd AT zouxianghui designanddevelopmentofarapidmeatdetectionsystembasedonrpacrisprcas12alfd AT zhuhui designanddevelopmentofarapidmeatdetectionsystembasedonrpacrisprcas12alfd AT zhaguangcai designanddevelopmentofarapidmeatdetectionsystembasedonrpacrisprcas12alfd AT sunjunjun designanddevelopmentofarapidmeatdetectionsystembasedonrpacrisprcas12alfd AT zhengyuzhong designanddevelopmentofarapidmeatdetectionsystembasedonrpacrisprcas12alfd AT linmin designanddevelopmentofarapidmeatdetectionsystembasedonrpacrisprcas12alfd |