Cargando…

New hybrid radio-fluorescent probes [(131)I]-BPF-01 and [(131)I]-BPF-02 for visualisation of cancer cells: Synthesis and preliminary in vitro and ex vivo evaluations

We synthesised and biologically evaluated two new hybrid probes [(131)I]BPF-01 and [(131)I]BPF-02 which were built from three structural entities: benzothiazole-phenyl, fluorescein isothiocyanate (FITC), and iodine-131. These probes were designed for potential applications in assisting surgical proc...

Descripción completa

Detalles Bibliográficos
Autores principales: Wongso, Hendris, Kurniawan, Ahmad, Forentin, Alfian M., Susilo, Veronika Y., Setiadi, Yanuar, Mahendra, Isa, Febrian, Muhamad B., Rosdianto, Aziiz M., Setiawan, Iwan, Goenawan, Hanna, Susianti, Susianti, Supratman, Unang, Widyasari, Eva M., Wibawa, Teguh H.A., Sriyani, Maula E., Halimah, Iim, Lesmana, Ronny
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10582398/
https://www.ncbi.nlm.nih.gov/pubmed/37860547
http://dx.doi.org/10.1016/j.heliyon.2023.e20710
_version_ 1785122321682399232
author Wongso, Hendris
Kurniawan, Ahmad
Forentin, Alfian M.
Susilo, Veronika Y.
Setiadi, Yanuar
Mahendra, Isa
Febrian, Muhamad B.
Rosdianto, Aziiz M.
Setiawan, Iwan
Goenawan, Hanna
Susianti, Susianti
Supratman, Unang
Widyasari, Eva M.
Wibawa, Teguh H.A.
Sriyani, Maula E.
Halimah, Iim
Lesmana, Ronny
author_facet Wongso, Hendris
Kurniawan, Ahmad
Forentin, Alfian M.
Susilo, Veronika Y.
Setiadi, Yanuar
Mahendra, Isa
Febrian, Muhamad B.
Rosdianto, Aziiz M.
Setiawan, Iwan
Goenawan, Hanna
Susianti, Susianti
Supratman, Unang
Widyasari, Eva M.
Wibawa, Teguh H.A.
Sriyani, Maula E.
Halimah, Iim
Lesmana, Ronny
author_sort Wongso, Hendris
collection PubMed
description We synthesised and biologically evaluated two new hybrid probes [(131)I]BPF-01 and [(131)I]BPF-02 which were built from three structural entities: benzothiazole-phenyl, fluorescein isothiocyanate (FITC), and iodine-131. These probes were designed for potential applications in assisting surgical procedures of solid cancers. The cytotoxicity study demonstrated that fluorescent probes BPF-01 (31.23 μg/mL) and BPF-02 (250 μg/mL) were relatively not toxic to normal immortalized human keratinocytes (HaCaT) cells, as indicated by the percentage of cell survival above 50 %. Furthermore, both probes displayed low to moderate anticancer activity against the breast cancer cells (MDA-MB-231) and prostate cancer cells (LNCaP and DU-145). The probe BPF-01 apparently showed an accumulation in the tumour tissues, as suggested by ex vivo fluorescence examinations. In addition, the cellular uptake study suggests that hybrid probe [(131)I]-BPF-01 was potentially accumulated in the MCF-7 cell line with the highest uptake of 16.11 ± 1.52 % after 2 h of incubation, approximately 50-fold higher than the accumulation of iodine-131 (control). The magnetic bead assay suggests that [(131)I]-BPF-02 and [(131)I]-BPF-02 showed a promising capability to interact with translocator protein 18 kDa (TSPO). Moreover, the computational data showed that the binding scores for ligands 7–8, BPF-01 and BPF-02, and [(131)I]-BPF-01 and [(131)I]-BPF-02 in the TSPO were considerably high. Accordingly, fluorescent probes BPF-01 and BPF-02, and hybrid probes [(131)I]BPF-01 and [(131)I]BPF-02 can be further developed for targeting cancer cells during intraoperative tumour surgery.
format Online
Article
Text
id pubmed-10582398
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Elsevier
record_format MEDLINE/PubMed
spelling pubmed-105823982023-10-19 New hybrid radio-fluorescent probes [(131)I]-BPF-01 and [(131)I]-BPF-02 for visualisation of cancer cells: Synthesis and preliminary in vitro and ex vivo evaluations Wongso, Hendris Kurniawan, Ahmad Forentin, Alfian M. Susilo, Veronika Y. Setiadi, Yanuar Mahendra, Isa Febrian, Muhamad B. Rosdianto, Aziiz M. Setiawan, Iwan Goenawan, Hanna Susianti, Susianti Supratman, Unang Widyasari, Eva M. Wibawa, Teguh H.A. Sriyani, Maula E. Halimah, Iim Lesmana, Ronny Heliyon Research Article We synthesised and biologically evaluated two new hybrid probes [(131)I]BPF-01 and [(131)I]BPF-02 which were built from three structural entities: benzothiazole-phenyl, fluorescein isothiocyanate (FITC), and iodine-131. These probes were designed for potential applications in assisting surgical procedures of solid cancers. The cytotoxicity study demonstrated that fluorescent probes BPF-01 (31.23 μg/mL) and BPF-02 (250 μg/mL) were relatively not toxic to normal immortalized human keratinocytes (HaCaT) cells, as indicated by the percentage of cell survival above 50 %. Furthermore, both probes displayed low to moderate anticancer activity against the breast cancer cells (MDA-MB-231) and prostate cancer cells (LNCaP and DU-145). The probe BPF-01 apparently showed an accumulation in the tumour tissues, as suggested by ex vivo fluorescence examinations. In addition, the cellular uptake study suggests that hybrid probe [(131)I]-BPF-01 was potentially accumulated in the MCF-7 cell line with the highest uptake of 16.11 ± 1.52 % after 2 h of incubation, approximately 50-fold higher than the accumulation of iodine-131 (control). The magnetic bead assay suggests that [(131)I]-BPF-02 and [(131)I]-BPF-02 showed a promising capability to interact with translocator protein 18 kDa (TSPO). Moreover, the computational data showed that the binding scores for ligands 7–8, BPF-01 and BPF-02, and [(131)I]-BPF-01 and [(131)I]-BPF-02 in the TSPO were considerably high. Accordingly, fluorescent probes BPF-01 and BPF-02, and hybrid probes [(131)I]BPF-01 and [(131)I]BPF-02 can be further developed for targeting cancer cells during intraoperative tumour surgery. Elsevier 2023-10-05 /pmc/articles/PMC10582398/ /pubmed/37860547 http://dx.doi.org/10.1016/j.heliyon.2023.e20710 Text en © 2023 The Authors. Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Wongso, Hendris
Kurniawan, Ahmad
Forentin, Alfian M.
Susilo, Veronika Y.
Setiadi, Yanuar
Mahendra, Isa
Febrian, Muhamad B.
Rosdianto, Aziiz M.
Setiawan, Iwan
Goenawan, Hanna
Susianti, Susianti
Supratman, Unang
Widyasari, Eva M.
Wibawa, Teguh H.A.
Sriyani, Maula E.
Halimah, Iim
Lesmana, Ronny
New hybrid radio-fluorescent probes [(131)I]-BPF-01 and [(131)I]-BPF-02 for visualisation of cancer cells: Synthesis and preliminary in vitro and ex vivo evaluations
title New hybrid radio-fluorescent probes [(131)I]-BPF-01 and [(131)I]-BPF-02 for visualisation of cancer cells: Synthesis and preliminary in vitro and ex vivo evaluations
title_full New hybrid radio-fluorescent probes [(131)I]-BPF-01 and [(131)I]-BPF-02 for visualisation of cancer cells: Synthesis and preliminary in vitro and ex vivo evaluations
title_fullStr New hybrid radio-fluorescent probes [(131)I]-BPF-01 and [(131)I]-BPF-02 for visualisation of cancer cells: Synthesis and preliminary in vitro and ex vivo evaluations
title_full_unstemmed New hybrid radio-fluorescent probes [(131)I]-BPF-01 and [(131)I]-BPF-02 for visualisation of cancer cells: Synthesis and preliminary in vitro and ex vivo evaluations
title_short New hybrid radio-fluorescent probes [(131)I]-BPF-01 and [(131)I]-BPF-02 for visualisation of cancer cells: Synthesis and preliminary in vitro and ex vivo evaluations
title_sort new hybrid radio-fluorescent probes [(131)i]-bpf-01 and [(131)i]-bpf-02 for visualisation of cancer cells: synthesis and preliminary in vitro and ex vivo evaluations
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10582398/
https://www.ncbi.nlm.nih.gov/pubmed/37860547
http://dx.doi.org/10.1016/j.heliyon.2023.e20710
work_keys_str_mv AT wongsohendris newhybridradiofluorescentprobes131ibpf01and131ibpf02forvisualisationofcancercellssynthesisandpreliminaryinvitroandexvivoevaluations
AT kurniawanahmad newhybridradiofluorescentprobes131ibpf01and131ibpf02forvisualisationofcancercellssynthesisandpreliminaryinvitroandexvivoevaluations
AT forentinalfianm newhybridradiofluorescentprobes131ibpf01and131ibpf02forvisualisationofcancercellssynthesisandpreliminaryinvitroandexvivoevaluations
AT susiloveronikay newhybridradiofluorescentprobes131ibpf01and131ibpf02forvisualisationofcancercellssynthesisandpreliminaryinvitroandexvivoevaluations
AT setiadiyanuar newhybridradiofluorescentprobes131ibpf01and131ibpf02forvisualisationofcancercellssynthesisandpreliminaryinvitroandexvivoevaluations
AT mahendraisa newhybridradiofluorescentprobes131ibpf01and131ibpf02forvisualisationofcancercellssynthesisandpreliminaryinvitroandexvivoevaluations
AT febrianmuhamadb newhybridradiofluorescentprobes131ibpf01and131ibpf02forvisualisationofcancercellssynthesisandpreliminaryinvitroandexvivoevaluations
AT rosdiantoaziizm newhybridradiofluorescentprobes131ibpf01and131ibpf02forvisualisationofcancercellssynthesisandpreliminaryinvitroandexvivoevaluations
AT setiawaniwan newhybridradiofluorescentprobes131ibpf01and131ibpf02forvisualisationofcancercellssynthesisandpreliminaryinvitroandexvivoevaluations
AT goenawanhanna newhybridradiofluorescentprobes131ibpf01and131ibpf02forvisualisationofcancercellssynthesisandpreliminaryinvitroandexvivoevaluations
AT susiantisusianti newhybridradiofluorescentprobes131ibpf01and131ibpf02forvisualisationofcancercellssynthesisandpreliminaryinvitroandexvivoevaluations
AT supratmanunang newhybridradiofluorescentprobes131ibpf01and131ibpf02forvisualisationofcancercellssynthesisandpreliminaryinvitroandexvivoevaluations
AT widyasarievam newhybridradiofluorescentprobes131ibpf01and131ibpf02forvisualisationofcancercellssynthesisandpreliminaryinvitroandexvivoevaluations
AT wibawateguhha newhybridradiofluorescentprobes131ibpf01and131ibpf02forvisualisationofcancercellssynthesisandpreliminaryinvitroandexvivoevaluations
AT sriyanimaulae newhybridradiofluorescentprobes131ibpf01and131ibpf02forvisualisationofcancercellssynthesisandpreliminaryinvitroandexvivoevaluations
AT halimahiim newhybridradiofluorescentprobes131ibpf01and131ibpf02forvisualisationofcancercellssynthesisandpreliminaryinvitroandexvivoevaluations
AT lesmanaronny newhybridradiofluorescentprobes131ibpf01and131ibpf02forvisualisationofcancercellssynthesisandpreliminaryinvitroandexvivoevaluations