Development of a sensitive microplate assay for characterizing RNA methyltransferase activity: Implications for epitranscriptomics and drug development

RNA methylation is a ubiquitous post-transcriptional modification found in diverse RNA classes and is a critical regulator of gene expression. In this study, we used Zika virus RNA methyltransferase (MTase) to develop a highly sensitive microplate assay that uses a biotinylated RNA substrate and rad...

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Detalles Bibliográficos
Autores principales: Mensah, Isaiah K., Norvil, Allison B., He, Ming, Lendy, Emma, Hjortland, Nicole, Tan, Hern, Pomerantz, Richard T., Mesecar, Andrew, Gowher, Humaira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10582764/
https://www.ncbi.nlm.nih.gov/pubmed/37716702
http://dx.doi.org/10.1016/j.jbc.2023.105257

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