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Mass spectrometry-based proteomic characterization of the middle-aged mouse brain for animal model research of neuromuscular diseases

Neuromuscular diseases with primary muscle wasting symptoms may also display multi-systemic changes in the body and exhibit secondary pathophysiological alterations in various non-muscle tissues. In some cases, this includes proteome-wide alterations and/or adaptations in the central nervous system....

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Autores principales: Dowling, Paul, Zweyer, Margit, Sabir, Hemmen, Henry, Michael, Meleady, Paula, Swandulla, Dieter, Ohlendieck, Kay
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PAGEPress Publications, Pavia, Italy 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583138/
https://www.ncbi.nlm.nih.gov/pubmed/37545360
http://dx.doi.org/10.4081/ejtm.2023.11553
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author Dowling, Paul
Zweyer, Margit
Sabir, Hemmen
Henry, Michael
Meleady, Paula
Swandulla, Dieter
Ohlendieck, Kay
author_facet Dowling, Paul
Zweyer, Margit
Sabir, Hemmen
Henry, Michael
Meleady, Paula
Swandulla, Dieter
Ohlendieck, Kay
author_sort Dowling, Paul
collection PubMed
description Neuromuscular diseases with primary muscle wasting symptoms may also display multi-systemic changes in the body and exhibit secondary pathophysiological alterations in various non-muscle tissues. In some cases, this includes proteome-wide alterations and/or adaptations in the central nervous system. Thus, in order to provide an improved bioanalytical basis for the comprehensive evaluation of animal models that are routinely used in muscle research, this report describes the mass spectrometry-based proteomic characterization of the mouse brain. Crude tissue extracts were examined by bottom-up proteomics and detected 4558 distinct protein species. The detailed analysis of the brain proteome revealed the presence of abundant cellular proteoforms in the neuronal cytoskeleton, as well as various brain region enriched proteins, including markers of the cerebral cortex, cerebellum, hippocampus and the olfactory bulb. Neuroproteomic markers of specific cell types in the brain were identified in association with various types of neurons and glia cells. Markers of subcellular structures were established for the plasmalemma, nucleus, endoplasmic reticulum, mitochondria and other crucial organelles, as well as synaptic components that are involved in presynaptic vesicle docking, neurotransmitter release and synapse remodelling.
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spelling pubmed-105831382023-10-19 Mass spectrometry-based proteomic characterization of the middle-aged mouse brain for animal model research of neuromuscular diseases Dowling, Paul Zweyer, Margit Sabir, Hemmen Henry, Michael Meleady, Paula Swandulla, Dieter Ohlendieck, Kay Eur J Transl Myol Article Neuromuscular diseases with primary muscle wasting symptoms may also display multi-systemic changes in the body and exhibit secondary pathophysiological alterations in various non-muscle tissues. In some cases, this includes proteome-wide alterations and/or adaptations in the central nervous system. Thus, in order to provide an improved bioanalytical basis for the comprehensive evaluation of animal models that are routinely used in muscle research, this report describes the mass spectrometry-based proteomic characterization of the mouse brain. Crude tissue extracts were examined by bottom-up proteomics and detected 4558 distinct protein species. The detailed analysis of the brain proteome revealed the presence of abundant cellular proteoforms in the neuronal cytoskeleton, as well as various brain region enriched proteins, including markers of the cerebral cortex, cerebellum, hippocampus and the olfactory bulb. Neuroproteomic markers of specific cell types in the brain were identified in association with various types of neurons and glia cells. Markers of subcellular structures were established for the plasmalemma, nucleus, endoplasmic reticulum, mitochondria and other crucial organelles, as well as synaptic components that are involved in presynaptic vesicle docking, neurotransmitter release and synapse remodelling. PAGEPress Publications, Pavia, Italy 2023-08-03 /pmc/articles/PMC10583138/ /pubmed/37545360 http://dx.doi.org/10.4081/ejtm.2023.11553 Text en Copyright © 2023, the Author(s) https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution NonCommercial 4.0 License (CC BY-NC 4.0).
spellingShingle Article
Dowling, Paul
Zweyer, Margit
Sabir, Hemmen
Henry, Michael
Meleady, Paula
Swandulla, Dieter
Ohlendieck, Kay
Mass spectrometry-based proteomic characterization of the middle-aged mouse brain for animal model research of neuromuscular diseases
title Mass spectrometry-based proteomic characterization of the middle-aged mouse brain for animal model research of neuromuscular diseases
title_full Mass spectrometry-based proteomic characterization of the middle-aged mouse brain for animal model research of neuromuscular diseases
title_fullStr Mass spectrometry-based proteomic characterization of the middle-aged mouse brain for animal model research of neuromuscular diseases
title_full_unstemmed Mass spectrometry-based proteomic characterization of the middle-aged mouse brain for animal model research of neuromuscular diseases
title_short Mass spectrometry-based proteomic characterization of the middle-aged mouse brain for animal model research of neuromuscular diseases
title_sort mass spectrometry-based proteomic characterization of the middle-aged mouse brain for animal model research of neuromuscular diseases
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583138/
https://www.ncbi.nlm.nih.gov/pubmed/37545360
http://dx.doi.org/10.4081/ejtm.2023.11553
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