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Successful 3D imaging of cleared biological samples with light sheet fluorescence microscopy
In parallel with the development of tissue-clearing methods, over the last decade, light sheet fluorescence microscopy has contributed to major advances in various fields, such as cell and developmental biology and neuroscience. While biologists are increasingly integrating three-dimensional imaging...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583220/ https://www.ncbi.nlm.nih.gov/pubmed/37847528 http://dx.doi.org/10.1083/jcb.202307143 |
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author | Delage, Elise Guilbert, Thomas Yates, Frank |
author_facet | Delage, Elise Guilbert, Thomas Yates, Frank |
author_sort | Delage, Elise |
collection | PubMed |
description | In parallel with the development of tissue-clearing methods, over the last decade, light sheet fluorescence microscopy has contributed to major advances in various fields, such as cell and developmental biology and neuroscience. While biologists are increasingly integrating three-dimensional imaging into their research projects, their experience with the technique is not always up to their expectations. In response to a survey of specific challenges associated with sample clearing and labeling, image acquisition, and data analysis, we have critically assessed the recent literature to characterize the difficulties inherent to light sheet fluorescence microscopy applied to cleared biological samples and to propose solutions to overcome them. This review aims to provide biologists interested in light sheet fluorescence microscopy with a primer for the development of their imaging pipeline, from sample preparation to image analysis. Importantly, we believe that issues could be avoided with better anticipation of image analysis requirements, which should be kept in mind while optimizing sample preparation and acquisition parameters. |
format | Online Article Text |
id | pubmed-10583220 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-105832202023-10-19 Successful 3D imaging of cleared biological samples with light sheet fluorescence microscopy Delage, Elise Guilbert, Thomas Yates, Frank J Cell Biol Review In parallel with the development of tissue-clearing methods, over the last decade, light sheet fluorescence microscopy has contributed to major advances in various fields, such as cell and developmental biology and neuroscience. While biologists are increasingly integrating three-dimensional imaging into their research projects, their experience with the technique is not always up to their expectations. In response to a survey of specific challenges associated with sample clearing and labeling, image acquisition, and data analysis, we have critically assessed the recent literature to characterize the difficulties inherent to light sheet fluorescence microscopy applied to cleared biological samples and to propose solutions to overcome them. This review aims to provide biologists interested in light sheet fluorescence microscopy with a primer for the development of their imaging pipeline, from sample preparation to image analysis. Importantly, we believe that issues could be avoided with better anticipation of image analysis requirements, which should be kept in mind while optimizing sample preparation and acquisition parameters. Rockefeller University Press 2023-10-17 /pmc/articles/PMC10583220/ /pubmed/37847528 http://dx.doi.org/10.1083/jcb.202307143 Text en © 2023 Delage et al. https://creativecommons.org/licenses/by/4.0/This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Delage, Elise Guilbert, Thomas Yates, Frank Successful 3D imaging of cleared biological samples with light sheet fluorescence microscopy |
title | Successful 3D imaging of cleared biological samples with light sheet fluorescence microscopy |
title_full | Successful 3D imaging of cleared biological samples with light sheet fluorescence microscopy |
title_fullStr | Successful 3D imaging of cleared biological samples with light sheet fluorescence microscopy |
title_full_unstemmed | Successful 3D imaging of cleared biological samples with light sheet fluorescence microscopy |
title_short | Successful 3D imaging of cleared biological samples with light sheet fluorescence microscopy |
title_sort | successful 3d imaging of cleared biological samples with light sheet fluorescence microscopy |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583220/ https://www.ncbi.nlm.nih.gov/pubmed/37847528 http://dx.doi.org/10.1083/jcb.202307143 |
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