Paramagnetic relaxivity of delocalized long-lived states of protons in chains of CH(2) groups

Long-lived states (LLSs) have lifetimes [Formula: see text] that can be much longer than longitudinal relaxation times [Formula: see text] . In molecules containing several geminal pairs of protons in neighboring CH [Formula: see text] groups, it has been shown that delocalized LLSs can be excited by converting magnetization into imbalances between the populations of singlet and triplet states of each pair. Since the empirical yield of the conversion and reconversion of observable magnetization into LLSs and back is on the order of 10 % if one uses spin-lock induced crossing (SLIC), it would be desirable to boost the sensitivity by dissolution dynamic nuclear polarization (d-DNP). To enhance the magnetization of nuclear spins by d-DNP, the analytes must be mixed with radicals such as 4-hydroxy-2,2,6,6-tetramethylpiperidin-1-oxyl (TEMPOL). After dissolution, these radicals lead to an undesirable paramagnetic relaxation enhancement (PRE) which shortens not only the longitudinal relaxation times [Formula: see text] but also the lifetimes [Formula: see text] of LLSs. It is shown in this work that PRE by TEMPOL is less deleterious for LLSs than for longitudinal magnetization for four different molecules: 2,2-dimethyl-2-silapentane-5-sulfonate (DSS), homotaurine, taurine, and acetylcholine. The relaxivities [Formula: see text] (i.e., the slopes of the relaxation rate constants [Formula: see text] as a function of the radical concentration) are 3 to 5 times smaller than the relaxivities [Formula: see text] of longitudinal magnetization. Partial delocalization of the LLSs across neighboring CH [Formula: see text] groups may decrease this advantage, but in practice, this effect was observed to be small, for example, when comparing taurine containing two CH [Formula: see text] groups and homotaurine with three CH [Formula: see text] groups. Regardless of whether the LLSs are delocalized or not, it is shown that PRE should not be a major problem for experiments combining d-DNP and LLSs, provided the concentration of paramagnetic species after dissolution does not exceed 1 mM, a condition that is readily fulfilled in typical d-DNP experiments. In bullet d-DNP experiments however, it may be necessary to decrease the concentration of TEMPOL or to add ascorbate for chemical reduction.