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Genes expression profiles in vascular cambium of Eucalyptus urophylla × Eucalyptus grandis at different ages

BACKGROUND: Wood is a secondary xylem generated by vascular cambium. Vascular cambium activities mainly include cambium proliferation and vascular tissue formation through secondary growth, thereby producing new secondary phloem inward and secondary xylem outward and leading to continuous tree thick...

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Autores principales: Liu, Guo, Wu, Zhihua, Luo, Jianzhong, Wang, Chubiao, Shang, Xiuhua, Zhang, Guowu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583469/
https://www.ncbi.nlm.nih.gov/pubmed/37848837
http://dx.doi.org/10.1186/s12870-023-04500-8
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author Liu, Guo
Wu, Zhihua
Luo, Jianzhong
Wang, Chubiao
Shang, Xiuhua
Zhang, Guowu
author_facet Liu, Guo
Wu, Zhihua
Luo, Jianzhong
Wang, Chubiao
Shang, Xiuhua
Zhang, Guowu
author_sort Liu, Guo
collection PubMed
description BACKGROUND: Wood is a secondary xylem generated by vascular cambium. Vascular cambium activities mainly include cambium proliferation and vascular tissue formation through secondary growth, thereby producing new secondary phloem inward and secondary xylem outward and leading to continuous tree thickening and wood formation. Wood formation is a complex biological process, which is strictly regulated by multiple genes. Therefore, molecular level research on the vascular cambium of different tree ages can lead to the identification of both key and related genes involved in wood formation and further explain the molecular regulation mechanism of wood formation. RESULTS: In the present study, RNA-Seq and Pac-Bio Iso-Seq were used for profiling gene expression changes in Eucalyptus urophylla × Eucalyptus grandis (E. urograndis) vascular cambium at four different ages. A total of 59,770 non-redundant transcripts and 1892 differentially expressed genes (DEGs) were identified. The expression trends of the DEGs related to cell division and differentiation, cell wall biosynthesis, phytohormone, and transcription factors were analyzed. The DEGs encoding expansin, kinesin, cycline, PAL, GRP9, KNOX, C2C2-dof, REV, etc., were highly expressed in E. urograndis at three years old, leading to positive effects on growth and development. Moreover, some gene family members, such as NAC, MYB, HD-ZIP III, RPK, and RAP, play different regulatory roles in wood formation because of their sophisticated transcriptional network and function redundantly. CONCLUSIONS: These candidate genes are a potential resource to further study wood formation, especially in fast-growing and adaptable eucalyptus. The results may also serve as a basis for further research to unravel the molecular mechanism underlying wood formation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04500-8.
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spelling pubmed-105834692023-10-19 Genes expression profiles in vascular cambium of Eucalyptus urophylla × Eucalyptus grandis at different ages Liu, Guo Wu, Zhihua Luo, Jianzhong Wang, Chubiao Shang, Xiuhua Zhang, Guowu BMC Plant Biol Research BACKGROUND: Wood is a secondary xylem generated by vascular cambium. Vascular cambium activities mainly include cambium proliferation and vascular tissue formation through secondary growth, thereby producing new secondary phloem inward and secondary xylem outward and leading to continuous tree thickening and wood formation. Wood formation is a complex biological process, which is strictly regulated by multiple genes. Therefore, molecular level research on the vascular cambium of different tree ages can lead to the identification of both key and related genes involved in wood formation and further explain the molecular regulation mechanism of wood formation. RESULTS: In the present study, RNA-Seq and Pac-Bio Iso-Seq were used for profiling gene expression changes in Eucalyptus urophylla × Eucalyptus grandis (E. urograndis) vascular cambium at four different ages. A total of 59,770 non-redundant transcripts and 1892 differentially expressed genes (DEGs) were identified. The expression trends of the DEGs related to cell division and differentiation, cell wall biosynthesis, phytohormone, and transcription factors were analyzed. The DEGs encoding expansin, kinesin, cycline, PAL, GRP9, KNOX, C2C2-dof, REV, etc., were highly expressed in E. urograndis at three years old, leading to positive effects on growth and development. Moreover, some gene family members, such as NAC, MYB, HD-ZIP III, RPK, and RAP, play different regulatory roles in wood formation because of their sophisticated transcriptional network and function redundantly. CONCLUSIONS: These candidate genes are a potential resource to further study wood formation, especially in fast-growing and adaptable eucalyptus. The results may also serve as a basis for further research to unravel the molecular mechanism underlying wood formation. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04500-8. BioMed Central 2023-10-18 /pmc/articles/PMC10583469/ /pubmed/37848837 http://dx.doi.org/10.1186/s12870-023-04500-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Liu, Guo
Wu, Zhihua
Luo, Jianzhong
Wang, Chubiao
Shang, Xiuhua
Zhang, Guowu
Genes expression profiles in vascular cambium of Eucalyptus urophylla × Eucalyptus grandis at different ages
title Genes expression profiles in vascular cambium of Eucalyptus urophylla × Eucalyptus grandis at different ages
title_full Genes expression profiles in vascular cambium of Eucalyptus urophylla × Eucalyptus grandis at different ages
title_fullStr Genes expression profiles in vascular cambium of Eucalyptus urophylla × Eucalyptus grandis at different ages
title_full_unstemmed Genes expression profiles in vascular cambium of Eucalyptus urophylla × Eucalyptus grandis at different ages
title_short Genes expression profiles in vascular cambium of Eucalyptus urophylla × Eucalyptus grandis at different ages
title_sort genes expression profiles in vascular cambium of eucalyptus urophylla × eucalyptus grandis at different ages
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10583469/
https://www.ncbi.nlm.nih.gov/pubmed/37848837
http://dx.doi.org/10.1186/s12870-023-04500-8
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