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The investigation of antibacterial properties of peptides and protein hydrolysates derived from serum of Asian water monitor (Varanus salvator)

It is well known that the Asian water monitors or Varanus salvator are both scavengers and predators. They can live and survive in the place that exposed to harmful microorganisms. Most people believe that they have some protected mechanisms to confront those infections. The aim of this study is to...

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Autores principales: Thanasak, Jitkamol, Roytrakul, Sittiruk, Toniti, Waraphan, Jaresitthikunchai, Janthima, Phaonakrop, Narumon, Thaisakun, Siriwan, Charoenlappanit, Sawanya, Surarit, Rudee, Sirimanapong, Wanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584125/
https://www.ncbi.nlm.nih.gov/pubmed/37851665
http://dx.doi.org/10.1371/journal.pone.0292947
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author Thanasak, Jitkamol
Roytrakul, Sittiruk
Toniti, Waraphan
Jaresitthikunchai, Janthima
Phaonakrop, Narumon
Thaisakun, Siriwan
Charoenlappanit, Sawanya
Surarit, Rudee
Sirimanapong, Wanna
author_facet Thanasak, Jitkamol
Roytrakul, Sittiruk
Toniti, Waraphan
Jaresitthikunchai, Janthima
Phaonakrop, Narumon
Thaisakun, Siriwan
Charoenlappanit, Sawanya
Surarit, Rudee
Sirimanapong, Wanna
author_sort Thanasak, Jitkamol
collection PubMed
description It is well known that the Asian water monitors or Varanus salvator are both scavengers and predators. They can live and survive in the place that exposed to harmful microorganisms. Most people believe that they have some protected mechanisms to confront those infections. The aim of this study is to determine the antibacterial activities of crude peptides and protein hydrolysates extracted from serum of the Varanus salvator. Ten types of bacteria were cultured with crude peptides and protein hydrolysates which were isolated from 21 Varanus salvator’s serum. The crude peptides showed some interested inhibition percentages against Enterobacter aerogenes ATCC13048 = 25.6%, Acinetobacter baumannii ATCC19606 = 33.4%, Burkholderia cepacia ATCC25416 = 35.3% and Pseudomonas aeruginosa ATCC27853 = 25.8%, whereas the protein hydrolysates had some inhibition potential on Burkholderia cepacia ATCC25416 = 24.3%. For the rest results of other tests were below 20% of inhibition. In addition, the evidences show that crude peptides have better antibacterial performances significantly than protein hydrolysates on most tested bacteria. Furthermore, antimicrobial peptides prediction shows about 10 percent hit (41/432 sequences). The interpretation shows that the best hit sequence is highly hydrophobic. It may destroy outer membrane of Gram-negative hence prevents the invasion of those bacteria. Altogether, bioinformatics and experiments show similar trends of antimicrobial peptide efficacy from Varanus salvator. Further studies need to be conducted on peptide purification and antimicrobial peptide candidate should be identified.
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spelling pubmed-105841252023-10-19 The investigation of antibacterial properties of peptides and protein hydrolysates derived from serum of Asian water monitor (Varanus salvator) Thanasak, Jitkamol Roytrakul, Sittiruk Toniti, Waraphan Jaresitthikunchai, Janthima Phaonakrop, Narumon Thaisakun, Siriwan Charoenlappanit, Sawanya Surarit, Rudee Sirimanapong, Wanna PLoS One Research Article It is well known that the Asian water monitors or Varanus salvator are both scavengers and predators. They can live and survive in the place that exposed to harmful microorganisms. Most people believe that they have some protected mechanisms to confront those infections. The aim of this study is to determine the antibacterial activities of crude peptides and protein hydrolysates extracted from serum of the Varanus salvator. Ten types of bacteria were cultured with crude peptides and protein hydrolysates which were isolated from 21 Varanus salvator’s serum. The crude peptides showed some interested inhibition percentages against Enterobacter aerogenes ATCC13048 = 25.6%, Acinetobacter baumannii ATCC19606 = 33.4%, Burkholderia cepacia ATCC25416 = 35.3% and Pseudomonas aeruginosa ATCC27853 = 25.8%, whereas the protein hydrolysates had some inhibition potential on Burkholderia cepacia ATCC25416 = 24.3%. For the rest results of other tests were below 20% of inhibition. In addition, the evidences show that crude peptides have better antibacterial performances significantly than protein hydrolysates on most tested bacteria. Furthermore, antimicrobial peptides prediction shows about 10 percent hit (41/432 sequences). The interpretation shows that the best hit sequence is highly hydrophobic. It may destroy outer membrane of Gram-negative hence prevents the invasion of those bacteria. Altogether, bioinformatics and experiments show similar trends of antimicrobial peptide efficacy from Varanus salvator. Further studies need to be conducted on peptide purification and antimicrobial peptide candidate should be identified. Public Library of Science 2023-10-18 /pmc/articles/PMC10584125/ /pubmed/37851665 http://dx.doi.org/10.1371/journal.pone.0292947 Text en © 2023 Thanasak et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Thanasak, Jitkamol
Roytrakul, Sittiruk
Toniti, Waraphan
Jaresitthikunchai, Janthima
Phaonakrop, Narumon
Thaisakun, Siriwan
Charoenlappanit, Sawanya
Surarit, Rudee
Sirimanapong, Wanna
The investigation of antibacterial properties of peptides and protein hydrolysates derived from serum of Asian water monitor (Varanus salvator)
title The investigation of antibacterial properties of peptides and protein hydrolysates derived from serum of Asian water monitor (Varanus salvator)
title_full The investigation of antibacterial properties of peptides and protein hydrolysates derived from serum of Asian water monitor (Varanus salvator)
title_fullStr The investigation of antibacterial properties of peptides and protein hydrolysates derived from serum of Asian water monitor (Varanus salvator)
title_full_unstemmed The investigation of antibacterial properties of peptides and protein hydrolysates derived from serum of Asian water monitor (Varanus salvator)
title_short The investigation of antibacterial properties of peptides and protein hydrolysates derived from serum of Asian water monitor (Varanus salvator)
title_sort investigation of antibacterial properties of peptides and protein hydrolysates derived from serum of asian water monitor (varanus salvator)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10584125/
https://www.ncbi.nlm.nih.gov/pubmed/37851665
http://dx.doi.org/10.1371/journal.pone.0292947
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